Strain Detail Sheet

Strain Name:
FVB/N-Tg(MMTV-Csnk2a1)1Dcs/Mmmh
Stock Number:
041878-MU
Citation ID:
RRID:MMRRC_041878-MU
Other Names:
MMTV-CK2a

Strain Information

Gene Details

Tg(MMTV-Csnk2a1)1Dcs
Name: transgene insertion, David C Seldin
Synonyms: MMTV-CK2alpha
Type: Allele
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic
Csnk2a1
Name: casein kinase 2, alpha 1 polypeptide
Synonyms: CK2, Csnk2a1-rs4
Type: Gene
Species: Mouse
Chromosome: 2
Alteration at locus: Transgenic
NCBI: 12995
Homologene: 90874
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Genetic Alterations
Murine CK2alpha catalytic subunit cDNA was subcloned into a vector in which the mammary tumor virus (MMTV) long terminal repeat (LTR) mainly directs expression to the mammary epithelium.
Genotype Determination

Strain Description

Phenotype

Homozygous: Thirty per cent of the female transgenic mice develop mammary adenocarcinomas at a median of 23 months of age.

Hemizygous: Thirty per cent of the female transgenic mice develop mammary adenocarcinomas at a median of 23 months of age.

MeSH Terms
  • Adenocarcinoma/etiology
  • Age of Onset
  • Animals
  • Breast Neoplasms/etiology
  • Casein Kinase II
  • Cell Transformation, Neoplastic
  • Cytoskeletal Proteins/metabolism
  • Female
  • Fibrocystic Breast Disease
  • Humans
  • Hyperplasia
  • Lactation
  • Mammary Glands, Animal/pathology
  • Mammary Neoplasms, Animal/chemically induced
  • Mammary Neoplasms, Animal/etiology
  • Mammary Tumor Virus, Mouse/genetics
  • Mice
  • Mice, Transgenic
  • NF-kappa B/metabolism
  • Protein-Serine-Threonine Kinases/biosynthesis
  • Protein-Serine-Threonine Kinases/genetics
  • Proto-Oncogene Proteins/metabolism
  • Proto-Oncogene Proteins c-myc/metabolism
  • Signal Transduction
  • Trans-Activators
  • Wnt Proteins
  • Zebrafish Proteins
  • beta Catenin
  • Blotting, Northern
  • Cell Line, Transformed
  • Cyclin D1/metabolism
  • DNA/metabolism
  • DNA, Complementary/metabolism
  • Dimerization
  • Immunoblotting
  • Luciferases/metabolism
  • Mammary Neoplasms, Animal/genetics
  • Mammary Neoplasms, Animal/virology
  • Neoplasm Metastasis
  • Promoter Regions, Genetic
  • Protein Structure, Tertiary
  • Proto-Oncogene Proteins c-bcl-2/genetics
  • Proto-Oncogene Proteins c-rel/genetics
  • RNA/metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Terminal Repeat Sequences
  • Time Factors
  • Transfection
  • Transgenes
  • Tumor Cells, Cultured
  • bcl-X Protein
  • 9,10-Dimethyl-1,2-benzanthracene/toxicity
  • Apigenin/pharmacology
  • Breast/cytology
  • Breast/drug effects
  • Breast/metabolism
  • Breast Neoplasms/enzymology
  • Breast Neoplasms/pathology
  • Carcinogens/toxicity
  • Casein Kinase II/antagonists & inhibitors
  • Casein Kinase II/physiology
  • Cell Movement
  • Collagen/metabolism
  • Drug Combinations
  • Enzyme Activation
  • Epithelial Cells/cytology
  • Epithelial Cells/drug effects
  • Epithelial Cells/metabolism
  • Gene Expression Regulation, Neoplastic
  • I-kappa B Kinase/metabolism
  • Laminin/metabolism
  • NF-kappa B/antagonists & inhibitors
  • Proteoglycans/metabolism
  • Proto-Oncogene Proteins c-rel/physiology
  • Transcription Factor RelB/metabolism
  • 3T3 Cells
  • Apoptosis/physiology
  • Breast Neoplasms/genetics
  • Cell Survival/physiology
  • Gene Amplification
  • Genes, erbB-2
  • I-kappa B Proteins/metabolism
  • Kidney/cytology
  • Kidney/enzymology
  • Mammary Neoplasms, Experimental/enzymology
  • Mammary Neoplasms, Experimental/genetics
  • Mammary Neoplasms, Experimental/pathology
  • NF-KappaB Inhibitor alpha
  • NF-kappa B/physiology
  • Protein-Serine-Threonine Kinases/antagonists & inhibitors
  • Protein-Serine-Threonine Kinases/metabolism
  • Protein-Serine-Threonine Kinases/physiology
  • Transcriptional Activation
  • 9,10-Dimethyl-1,2-benzanthracene
  • Adenocarcinoma/chemically induced
  • Adenocarcinoma/genetics
  • Adenocarcinoma/pathology
  • Beverages
  • Carcinoma in Situ/chemically induced
  • Carcinoma in Situ/genetics
  • Carcinoma in Situ/pathology
  • Flavonoids/pharmacology
  • Mammary Neoplasms, Animal/pathology
  • Neoplasm Invasiveness/genetics
  • Phenols/pharmacology
  • Phenotype
  • Polyphenols
  • Rats
  • Rats, Sprague-Dawley
  • Snail Family Transcription Factors
  • Transcription Factors/genetics
  • Breast/pathology
  • Calcium-Calmodulin-Dependent Protein Kinases/metabolism
  • Cell Transformation, Neoplastic/metabolism
  • Cell Transformation, Neoplastic/pathology
  • Cyclin D1/genetics
  • DNA-Binding Proteins/metabolism
  • Fibroblast Growth Factor 3
  • Fibroblast Growth Factors/genetics
  • Fibroblast Growth Factors/metabolism
  • Glycogen Synthase Kinase 3
  • Immunoenzyme Techniques
  • Keratins/metabolism
  • Lymphoid Enhancer-Binding Factor 1
  • Mammary Neoplasms, Experimental/metabolism
  • Proto-Oncogene Proteins/genetics
  • Trans-Activators/metabolism
  • Transcription Factors/metabolism
  • Up-Regulation
  • Cell Differentiation/genetics
  • Cell Transformation, Neoplastic/genetics
  • Genes, erbB
  • Genes, ras
  • Signal Transduction/genetics
  • Wnt1 Protein
Strain Development
One-cell zygotes that were microinjected with the transgene were reimplanted into pseudopregnant foster mothers. Positive transgene carriers were bred to establish this transgenic line in the FVB/N background.
Suggested Control Mice
Wild-type littermates

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.

Research Applications

  • Apoptosis
  • Cancer
  • Cell Biology
  • Models for Human Disease
  • Research Tools

Strain Origin

Donor
David C. Seldin, M.D., Ph.D., Boston University.
Esther Landesman, Ph.D., BU.
Primary Reference

Landesman-Bollag E, Romieu-Mourez R, Song DH, Sonenshein GE, Cardiff RD, Seldin DC. Protein kinase CK2 in mammary gland tumorigenesis. Oncogene. 2001 May 31;20(25):3247-57. (Medline PMID: 11423974)

Romieu-Mourez R, Kim DW, Shin SM, Demicco EG, Landesman-Bollag E, Seldin DC, Cardiff RD, and Sonenshein GE. Mouse mammary tumor virus c-rel transgenic mice develop mammary tumors. Mol Cell Biol. 2003 Aug; 23(16): 5738-5754. (Medline PMID: 12897145)

Eddy SF, Guo S, Demicco EG, Romieu-Mourez R, Landesman-Bollag E, Seldin DC, Sonenshein GE. Inducible IkappaB kinase/IkappaB kinase epsilon expression is induced by CK2 and promotes aberrant nuclear factor-kappaB activation in breast cancer cells. Cancer Res. 2005 Dec 15;65(24):11375-83. (Medline PMID: 16357145)

Romieu-Mourez R, Landesman-Bollag E, Seldin DC, Sonenshein GE. Protein kinase CK2 promotes aberrant activation of nuclear factor-kappaB, transformed phenotype, and survival of breast cancer cells. Cancer Res. 2002 Nov 15;62(22):6770-8. (Medline PMID: 12438279)

Belguise K, Guo S, Yang S, Rogers AE, Seldin DC, Sherr DH, Sonenshein GE. Green tea polyphenols reverse cooperation between c-Rel and CK2 that induces the aryl hydrocarbon receptor, slug, and an invasive phenotype. Cancer Res. 2007 Dec 15;67(24):11742-50. (Medline PMID: 18089804)

Miyoshi K, Rosner A, Nozawa M, Byrd C, Morgan F, Landesman-Bollag E, Xu X, Seldin DC, Schmidt EV, Taketo MM, Robinson GW, Cardiff RD, Hennighausen L. Activation of different Wnt/beta-catenin signaling components in mammary epithelium induces transdifferentiation and the formation of pilar tumors. Oncogene. 2002 Aug 15;21(36):5548-56. (Medline PMID: 12165853)

Rosner A, Miyoshi K, Landesman-Bollag E, Xu X, Seldin DC, Moser AR, MacLeod CL, Shyamala G, Gillgrass AE, Cardiff RD. Pathway pathology: histological differences between ErbB/Ras and Wnt pathway transgenic mammary tumors. Am J Pathol. 2002 Sep;161(3):1087-97. (Medline PMID: 12213737)

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
white

Breeding

MMRRC Breeding System
Sib-mating
Breeding Scheme(s)
  • Wild-type female x Hemizygous male littermate or reciprocal mating
  • Hemizygous female x Hemizygous male littermate
  • Homozygous female x Homozygous male littermate
Overall Breeding Performance
Excellent

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 8 to 12 months > 12 months
Average litter size
5-9
Recommended wean age
3 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.