Strain Name:
B6;SJL-Prop1df Tg(PROP1)884Sac/Mmnc
Stock Number:
041882-UNC
Citation ID:
RRID:MMRRC_041882-UNC
Other Names:
HsProp1

Strain Information

Prop1df
Name: paired like homeodomain factor 1; Ames dwarf
Synonyms: DW, Prop1-, df, Amesdf
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Spontaneous Mutation
Prop1
Name: paired like homeodomain factor 1
Synonyms: prophet of Pit-1, prophet of Pit1, Prop-1
Type: Gene
Species: Mouse
Chromosome: 11
Alteration at locus: Spontaneous Mutation
NCBI: 19127
HGNC: HGNC:9455
Homologene: 4558
PROP1
Name: PROP paired-like homeobox 1
Type: Gene
Species: Homo sapiens (human)
Chromosome: 5
Alteration at locus: Spontaneous Mutation
Tg(PROP1)884Sac
Name:
Type: Transgene
Species: Mus musculus (mouse)
Chromosome:
Alteration at locus: Spontaneous Mutation
Genetic Alterations
BAC RP11–452O4 containing 175 kb spanning the human PROP1 genomic locus was obtained from BACPAC Resources, expanded, and purified. Purified BAC (HsPROP1) was injected into the pronuclei of fertilized eggs. The insertion site has not been determined.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • Phenotype

    Homozygous: Not characterized

    Hemizygous: Hemizygous mice are viable and fertile. Detailed phenotyping has not been performed, but is not expected to differ from wild type.

    Mammalian Phenotype Terms
    Allelic Composition: (Genetic Background: )

    Strain Development
    Founder mice were crossed with heterozygous Prop1df mice to demonstrate that human PROP1 rescues the mouse Prop1df dwarf phenotype.  Males containing the Prop1df allele and the transgene were maintained by crossing with C57BL/6J.
    Suggested Control Mice
    Wild-type littermates
    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.
    • Cell Biology
    • Developmental Biology
    • Endocrine Deficiency
    • Internal Organ
    • Models for Human Disease
    • Research Tools
    Donor
    Shannon Davis, Ph.D., University of South Carolina.

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.
    Coat Color
    Black
    MMRRC Breeding System
    Backcross
    Breeding Scheme(s)
    • Wild-type (C57BL/6J) female x Hemizygous male or reciprocal mating
    Generation
    3
    Overall Breeding Performance
    Undetermined
    NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Undetermined Undetermined
    Homozygotes are fertile: Undetermined Undetermined
    Hetero/Hemizygotes are fertile: Yes Yes
    Age Reproductive Decline: Undetermined Undetermined
    Bred to Homozygosity
    No
    Average litter size
    4-6
    Recommended wean age
    3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    041882-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.