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Strain Name:
C57BL/6J-MtgxR4623Btlr/Mmmh
Stock Number:
041888-MU
Citation ID:
RRID:MMRRC_041888-MU
Other Names:
R4623 (G1), C57BL/6J-MtgxR4623Btlr
Major Collection:

Strain Information

Pik3r1
Name: phosphoinositide-3-kinase regulatory subunit 1
Synonyms: p85alpha, p50alpha, p55alpha, PI3K
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 18708
HGNC: HGNC:8979
Homologene: 7889
Ppp1r16b
Name: protein phosphatase 1, regulatory subunit 16B
Synonyms: ANKRD4, Wdt4, C130078N17Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228852
Homologene: 9194
Calb1
Name: calbindin 1
Synonyms: Brain-2, Calb-1, Calb, CB, calbindin-D (28k), calbindin, CalbindinD28K
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 12307
HGNC: HGNC:1434
Homologene: 21026
Gsr
Name: glutathione reductase
Synonyms: Gr-1, Gr1, D8Ertd238e
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 14782
HGNC: HGNC:4623
Homologene: 531
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Pds5b
Name: PDS5 cohesin associated factor B
Synonyms: AS3, Aprin
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100710
Homologene: 41001
Neurl4
Name: neuralized E3 ubiquitin protein ligase 4
Synonyms: 0610025P10Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216860
Homologene: 13029
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 16,131,063 bp
  • A to T, chromosome 1 at 59,556,276 bp
  • C to A, chromosome 1 at 94,009,456 bp
  • A to T, chromosome 1 at 194,812,150 bp
  • A to G, chromosome 2 at 31,396,710 bp
  • A to T, chromosome 2 at 41,246,021 bp
  • T to A, chromosome 2 at 86,754,562 bp
  • A to G, chromosome 2 at 158,761,463 bp
  • T to C, chromosome 3 at 28,029,244 bp
  • T to A, chromosome 3 at 124,321,860 bp
  • A to T, chromosome 3 at 126,364,796 bp
  • T to C, chromosome 4 at 9,622,005 bp
  • T to A, chromosome 4 at 15,895,721 bp
  • C to T, chromosome 4 at 24,502,792 bp
  • A to G, chromosome 4 at 34,719,159 bp
  • G to A, chromosome 4 at 94,863,661 bp
  • A to T, chromosome 4 at 107,190,718 bp
  • A to G, chromosome 4 at 118,486,611 bp
  • A to G, chromosome 4 at 123,372,348 bp
  • A to G, chromosome 4 at 129,720,842 bp
  • A to G, chromosome 4 at 132,303,193 bp
  • T to C, chromosome 4 at 154,667,290 bp
  • A to G, chromosome 5 at 24,348,442 bp
  • T to C, chromosome 5 at 114,260,930 bp
  • T to A, chromosome 5 at 118,232,406 bp
  • G to A, chromosome 5 at 131,440,383 bp
  • T to C, chromosome 5 at 136,308,300 bp
  • T to A, chromosome 5 at 149,214,595 bp
  • T to A, chromosome 5 at 150,800,601 bp
  • C to A, chromosome 6 at 120,961,625 bp
  • T to C, chromosome 6 at 121,325,145 bp
  • A to G, chromosome 6 at 125,173,609 bp
  • T to A, chromosome 6 at 140,551,186 bp
  • A to T, chromosome 7 at 16,673,466 bp
  • G to T, chromosome 7 at 26,933,491 bp
  • A to G, chromosome 7 at 50,120,562 bp
  • G to T, chromosome 7 at 64,373,553 bp
  • T to C, chromosome 7 at 78,791,941 bp
  • A to G, chromosome 7 at 110,267,872 bp
  • A to C, chromosome 8 at 4,739,178 bp
  • C to A, chromosome 8 at 24,438,709 bp
  • A to G, chromosome 8 at 33,680,305 bp
  • A to G, chromosome 8 at 88,589,755 bp
  • T to A, chromosome 9 at 59,871,072 bp
  • T to A, chromosome 9 at 90,186,462 bp
  • T to C, chromosome 9 at 94,520,398 bp
  • T to C, chromosome 9 at 107,062,045 bp
  • A to T, chromosome 10 at 14,441,499 bp
  • T to G, chromosome 10 at 60,300,851 bp
  • A to G, chromosome 10 at 80,782,150 bp
  • A to G, chromosome 10 at 93,507,439 bp
  • A to G, chromosome 10 at 117,670,297 bp
  • A to G, chromosome 10 at 128,641,119 bp
  • G to A, chromosome 10 at 129,388,046 bp
  • A to G, chromosome 11 at 9,309,130 bp
  • A to G, chromosome 11 at 17,262,742 bp
  • A to T, chromosome 11 at 58,316,455 bp
  • A to G, chromosome 11 at 67,286,258 bp
  • C to A, chromosome 11 at 69,907,461 bp
  • T to C, chromosome 11 at 71,161,843 bp
  • T to C, chromosome 11 at 78,325,121 bp
  • T to C, chromosome 12 at 11,222,140 bp
  • A to T, chromosome 12 at 64,982,450 bp
  • A to G, chromosome 12 at 84,809,348 bp
  • A to T, chromosome 13 at 61,202,160 bp
  • C to T, chromosome 13 at 95,041,939 bp
  • T to C, chromosome 13 at 101,692,262 bp
  • A to T, chromosome 14 at 21,014,939 bp
  • A to C, chromosome 14 at 21,743,478 bp
  • G to A, chromosome 14 at 44,989,014 bp
  • T to C, chromosome 14 at 70,522,278 bp
  • T to A, chromosome 14 at 121,912,167 bp
  • T to A, chromosome 15 at 66,735,271 bp
  • C to T, chromosome 15 at 76,372,468 bp
  • C to A, chromosome 15 at 99,626,206 bp
  • A to T, chromosome 15 at 102,157,709 bp
  • C to T, chromosome 16 at 19,883,737 bp
  • T to A, chromosome 16 at 20,681,345 bp
  • A to T, chromosome 16 at 58,824,106 bp
  • A to G, chromosome 17 at 43,450,983 bp
  • C to A, chromosome 17 at 57,299,839 bp
  • A to G, chromosome 17 at 78,795,119 bp
  • C to A, chromosome 18 at 10,808,086 bp
  • T to A, chromosome 18 at 32,127,473 bp
  • A to T, chromosome 19 at 37,409,747 bp
  • C to T, chromosome 19 at 61,175,926 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4623 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041888-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.