Strain Name:
C57BL/6J-MtgxR4626Btlr/Mmmh
Stock Number:
041891-MU
Citation ID:
RRID:MMRRC_041891-MU
Other Names:
R4626 (G1), C57BL/6J-MtgxR4626Btlr
Major Collection:

Strain Information

Aatf
Name: apoptosis antagonizing transcription factor
Synonyms: 4933415H02Rik, Trb, 5830465M17Rik, Che-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 56321
Homologene: 40811
Stub1
Name: STIP1 homology and U-Box containing protein 1
Synonyms: CHIP, 0610033N24Rik, 2210017D18Rik, 2310040B03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 56424
VEGA: 17
Homologene: 4281
Pomt1
Name: protein-O-mannosyltransferase 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 99011
HGNC: HGNC:9202
Homologene: 68548
Arhgap39
Name: Rho GTPase activating protein 39
Synonyms: D15Wsu169e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 223666
Homologene: 27825
Snx10
Name: sorting nexin 10
Synonyms: 2410004M09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 71982
Homologene: 32171
Kcnq2
Name: potassium voltage-gated channel, subfamily Q, member 2
Synonyms: KQT2, Nmf134
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 16536
HGNC: HGNC:6296
Homologene: 26174
Tfr2
Name: transferrin receptor 2
Synonyms: Tfr2, Trfr2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 50765
Homologene: 2428
Wdfy3
Name: WD repeat and FYVE domain containing 3
Synonyms: Ggtb3, 2610509D04Rik, D5Ertd66e, Bwf1, Bchs, Alfy
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 72145
Homologene: 22855
Mtdh
Name: metadherin
Synonyms: D8Bwg1112e, 3D3/Lyric, Lyric, 2610103J23Rik, AEG-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 67154
Homologene: 12089
Ap3b1
Name: adaptor-related protein complex 3, beta 1 subunit
Synonyms: rim2, recombination induced mutation 2, Hps2, beta3A, AP-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 11774
VEGA: 13
HGNC: HGNC:566
Homologene: 68125
Nup214
Name: nucleoporin 214
Synonyms: D2H9S46E, CAN
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 227720
HGNC: HGNC:8064
Homologene: 38008
Orc5
Name: origin recognition complex, subunit 5
Synonyms: mouse origin recognition complex 5, MmORC5, Orc5l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 26429
HGNC: HGNC:8491
Homologene: 37636
Ncapd2
Name: non-SMC condensin I complex, subunit D2
Synonyms: CNAP1, CAP-D2, 2810406C15Rik, 2810465G24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 68298
Homologene: 6497
Scn10a
Name: sodium channel, voltage-gated, type X, alpha
Synonyms: Nav1.8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 20264
VEGA: 9
Homologene: 21300
Lama5
Name: laminin, alpha 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 16776
HGNC: HGNC:6485
Homologene: 4060
Pole
Name: polymerase (DNA directed), epsilon
Synonyms: pol-epsilon
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 18973
HGNC: HGNC:9177
Homologene: 4539
Abca17
Name: ATP-binding cassette, sub-family A member 17
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 381072
Homologene: 86807
D5Ertd579e
Name: DNA segment, Chr 5, ERATO Doi 579, expressed
Synonyms: 9030221A05Rik, A930018H20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 320661
Homologene: 19716
Gpc6
Name: glypican 6
Synonyms: 6720429C22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 23888
HGNC: HGNC:4454
Homologene: 55922
Vav2
Name: vav 2 oncogene
Synonyms: 2810040F13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22325
Homologene: 2530
Mcm6
Name: minichromosome maintenance complex component 6
Synonyms: Mcmd6, D1Wsu22e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 17219
HGNC: HGNC:6949
Homologene: 4322
Bccip
Name: BRCA2 and CDKN1A interacting protein
Synonyms: TOK-1, 1110013J05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 66165
HGNC: HGNC:978
Homologene: 41629
Btbd10
Name: BTB domain containing 10
Synonyms: Gmrp1, 1110056N09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 68815
Homologene: 13009
Nup58
Name: nucleoporin 58
Synonyms: 1700017F11Rik, Nupl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 71844
VEGA: 14
Homologene: 40924
Csnk1g2
Name: casein kinase 1, gamma 2
Synonyms: 2810429I12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 103236
VEGA: 10
HGNC: HGNC:2455
Homologene: 100845
Zfyve26
Name: zinc finger, FYVE domain containing 26
Synonyms: 9330197E15Rik, LOC380767, A630028O16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 211978
VEGA: 12
Homologene: 9102
Mis18bp1
Name: MIS18 binding protein 1
Synonyms: C79407
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 217653
Homologene: 10147
Zcchc7
Name: zinc finger, CCHC domain containing 7
Synonyms: 4930572I07Rik, D4Wsu132e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 319885
Homologene: 22217
Peg10
Name: paternally expressed 10
Synonyms: MEF3L, HB-1, MyEF-3, MyEF-3 like, Edr, Mart2, Mar2, Rtl2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 170676
Homologene: 116067
Fhdc1
Name: FH2 domain containing 1
Synonyms: 6330505N24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229474
Homologene: 18920
F2
Name: coagulation factor II
Synonyms: prothrombin, Cf-2, Cf2, FII, thrombin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 14061
HGNC: HGNC:3535
Homologene: 426
Zfhx4
Name: zinc finger homeodomain 4
Synonyms: Zfh-4, C130041O22Rik, Zfh4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 80892
Homologene: 23477
Atp1a3
Name: ATPase, Na+/K+ transporting, alpha 3 polypeptide
Synonyms: Atpa-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 232975
HGNC: HGNC:801
Homologene: 113729
Fbn2
Name: fibrillin 2
Synonyms: sy, Sne, Fib-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 14119
VEGA: 18
HGNC: HGNC:3604
Homologene: 1515
Lrguk
Name: leucine-rich repeats and guanylate kinase domain containing
Synonyms: 4921528H16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 74354
Homologene: 34923
Akr1c13
Name: aldo-keto reductase family 1, member C13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 27384
Homologene: 121208
Iba57
Name: IBA57 homolog, iron-sulfur cluster assembly
Synonyms: 4930543L23Rik, A230051G13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 216792
Homologene: 13844
Htra4
Name: HtrA serine peptidase 4
Synonyms: B430206E18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 330723
Homologene: 17801
Grm5
Name: glutamate receptor, metabotropic 5
Synonyms: mGluR5, Gprc1e, 6430542K11Rik, Glu5R
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 108071
HGNC: HGNC:4597
Homologene: 37354
Gys1
Name: glycogen synthase 1, muscle
Synonyms: Gys3, MGS
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 14936
HGNC: HGNC:4706
Homologene: 113557
Mindy2
Name: MINDY lysine 48 deubiquitinase 2
Synonyms: B230380D07Rik, Fam63b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235461
VEGA: 9
Homologene: 101023
Atp9a
Name: ATPase, class II, type 9A
Synonyms: Class II, IIa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 11981
Homologene: 69194
Adamts18
Name: ADAM metallopeptidase with thrombospondin type 1 motif 18
Synonyms: E130314N14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 208936
Homologene: 65241
Cacna1e
Name: calcium channel, voltage-dependent, R type, alpha 1E subunit
Synonyms: Cav2.3, Cchra1, alpha1E
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12290
HGNC: HGNC:1392
Homologene: 20185
Kctd21
Name: potassium channel tetramerisation domain containing 21
Synonyms: EG622320
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 622320
Homologene: 54754
Atxn1
Name: ataxin 1
Synonyms: Atx1, Sca1, 2900016G23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 20238
VEGA: 13
Homologene: 281
Ranbp6
Name: RAN binding protein 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 240614
HGNC: HGNC:9851
Homologene: 18935
Pcdhb9
Name: protocadherin beta 9
Synonyms: Pcdhb4C, PcdhbI
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 93880
HGNC: HGNC:8689
Homologene: 87124
Galnt13
Name: polypeptide N-acetylgalactosaminyltransferase 13
Synonyms: pp-GalNAc-T13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 271786
Homologene: 62167
Mettl18
Name: methyltransferase like 18
Synonyms: 2810422O20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 69962
Homologene: 32680
Pdzd7
Name: PDZ domain containing 7
Synonyms: Pdzk7, EG435601
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 100503041
Homologene: 129509
Poll
Name: polymerase (DNA directed), lambda
Synonyms: 1110003P06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 56626
VEGA: 19
HGNC: HGNC:9184
Homologene: 40863
Alkbh2
Name: alkB homolog 2, alpha-ketoglutarate-dependent dioxygenase
Synonyms: Abh2, mABH2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231642
Homologene: 18393
Ugt2b36
Name: UDP glucuronosyltransferase 2 family, polypeptide B36
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231396
Homologene: 128251
Slc22a22
Name: solute carrier family 22 (organic cation transporter), member 22
Synonyms: OAT-PG, BC026439
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 210463
Homologene: 18166
Zp3r
Name: zona pellucida 3 receptor
Synonyms: SP56
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 22789
HGNC: HGNC:1325
Homologene: 7609
Use1
Name: unconventional SNARE in the ER 1 homolog (S. cerevisiae)
Synonyms: 5730403H22Rik, D12, Q-SNARE, mED2, 2010315L10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 67023
Homologene: 10214
Krt1
Name: keratin 1
Synonyms: Krt2-1, Krt-2.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 16678
VEGA: 15
HGNC: HGNC:6412
Homologene: 38146
Cfhr2
Name: complement factor H-related 2
Synonyms: FHR-B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 545366
Homologene: 134349
Or1j1
Name: olfactory receptor family 1 subfamily J member 1
Synonyms: Y71, MOR136-14, GA_x6K02T2NLDC-33507606-33506665, Olfr3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 18328
HGNC: HGNC:8208
Homologene: 66579
Or6f2
Name: olfactory receptor family 6 subfamily F member 2
Synonyms: GA_x6K02T2PBJ9-42327937-42328872, MOR104-4, Olfr523
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258511
Homologene: 27191
Trmu
Name: tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase
Synonyms: 1600025P05Rik, 1110005N20Rik, Trmt1, Mtu1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 72026
Homologene: 5589
H2-T27
Name: histocompatibility 2, T region locus 27
Synonyms: Gm11127
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 100529082
Homologene: 133121
Prr16
Name: proline rich 16
Synonyms: 5430406M13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 71373
VEGA: 18
Homologene: 41152
Or4c101
Name: olfactory receptor family 4 subfamily C member 101
Synonyms: GA_x6K02T2Q125-50046879-50047784, MOR238-2, Olfr1188
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258921
Ankrd49
Name: ankyrin repeat domain 49
Synonyms: Gbif
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 56503
VEGA: 9
Homologene: 9787
Ptprv
Name: protein tyrosine phosphatase receptor type V
Synonyms: mOST-PTP, Esp, OST-PTP, OST
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 13924
Homologene: 7306
Rmi1
Name: RecQ mediated genome instability 1
Synonyms: 4932432N11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 74386
VEGA: 13
Homologene: 41601
Gm28417
Name: predicted gene 28417
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 118567734
Pcdha10
Name: protocadherin alpha 10
Synonyms: Cnr8, Cnr3, Crnr8, Crnr3, Pcdha14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 12943
VEGA: 18
HGNC: HGNC:8670
Homologene: 135720
Gdpd2
Name: glycerophosphodiester phosphodiesterase domain containing 2
Synonyms: GDE3, 9130017L10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 71584
Homologene: 9792
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 34,471,792 bp
  • T to C, chromosome 1 at 128,351,548 bp
  • A to G, chromosome 1 at 130,615,175 bp
  • T to C, chromosome 1 at 135,124,131 bp
  • A to T, chromosome 1 at 139,813,576 bp
  • T to C, chromosome 1 at 154,482,548 bp
  • T to A, chromosome 1 at 163,996,476 bp
  • T to C, chromosome 2 at 27,270,160 bp
  • T to A, chromosome 2 at 32,033,404 bp
  • A to G, chromosome 2 at 32,254,412 bp
  • A to G, chromosome 2 at 36,812,259 bp
  • T to A, chromosome 2 at 54,857,866 bp
  • T to C, chromosome 2 at 88,559,832 bp
  • T to A, chromosome 2 at 91,630,670 bp
  • T to C, chromosome 2 at 168,639,943 bp
  • G to A, chromosome 2 at 180,184,460 bp
  • A to T, chromosome 2 at 181,085,399 bp
  • T to A, chromosome 3 at 5,402,639 bp
  • C to T, chromosome 3 at 84,474,250 bp
  • T to C, chromosome 4 at 44,931,289 bp
  • G to T, chromosome 5 at 22,548,005 bp
  • T to C, chromosome 5 at 36,614,559 bp
  • A to C, chromosome 5 at 87,092,088 bp
  • A to G, chromosome 5 at 101,943,934 bp
  • A to G, chromosome 5 at 110,317,861 bp
  • C to T, chromosome 5 at 114,124,226 bp
  • T to C, chromosome 5 at 137,571,692 bp
  • GGATCC to GGATCCCCATCAAGATCC, chromosome 6 at 4,756,460 bp
  • G to A, chromosome 6 at 34,129,223 bp
  • A to G, chromosome 6 at 51,588,290 bp
  • T to C, chromosome 6 at 125,168,246 bp
  • T to A, chromosome 7 at 24,998,768 bp
  • T to C, chromosome 7 at 45,439,534 bp
  • G to T, chromosome 7 at 88,130,153 bp
  • A to G, chromosome 7 at 97,347,575 bp
  • T to C, chromosome 7 at 113,328,398 bp
  • T to C, chromosome 7 at 133,720,728 bp
  • T to C, chromosome 7 at 140,176,446 bp
  • T to C, chromosome 8 at 25,037,114 bp
  • G to T, chromosome 8 at 71,367,324 bp
  • C to T, chromosome 8 at 113,773,168 bp
  • A to C, chromosome 9 at 14,782,640 bp
  • G to A, chromosome 9 at 70,626,781 bp
  • A to G, chromosome 9 at 119,631,505 bp
  • C to A, chromosome 10 at 80,639,814 bp
  • A to G, chromosome 11 at 59,158,461 bp
  • A to C, chromosome 11 at 84,422,958 bp
  • G to T, chromosome 12 at 65,140,766 bp
  • T to A, chromosome 12 at 79,269,070 bp
  • G to T, chromosome 13 at 4,197,870 bp
  • T to C, chromosome 13 at 45,567,099 bp
  • A to G, chromosome 13 at 58,409,136 bp
  • T to A, chromosome 13 at 94,404,078 bp
  • A to G, chromosome 14 at 60,238,555 bp
  • C to A, chromosome 14 at 117,964,843 bp
  • C to T, chromosome 15 at 34,114,834 bp
  • T to C, chromosome 15 at 57,263,338 bp
  • A to C, chromosome 15 at 76,737,637 bp
  • T to C, chromosome 15 at 85,894,985 bp
  • C to T, chromosome 15 at 101,846,187 bp
  • A to T, chromosome 17 at 24,321,084 bp
  • A to G, chromosome 17 at 25,831,871 bp
  • CTGGGTG to CTG, chromosome 17 at 36,057,896 bp
  • A to T, chromosome 18 at 37,006,998 bp
  • T to A, chromosome 18 at 37,402,249 bp
  • C to T, chromosome 18 at 51,302,839 bp
  • A to T, chromosome 18 at 58,013,747 bp
  • A to T, chromosome 19 at 29,810,863 bp
  • A to T, chromosome 19 at 45,029,762 bp
  • C to A, chromosome 19 at 45,555,124 bp
  • T to C, chromosome X at 100,734,148 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4626 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041891-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.