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Strain Name:
C57BL/6J-MtgxR4651Btlr/Mmmh
Stock Number:
041911-MU
Citation ID:
RRID:MMRRC_041911-MU
Other Names:
R4651 (G1), C57BL/6J-MtgxR4651Btlr
Major Collection:

Strain Information

Gad2
Name: glutamic acid decarboxylase 2
Synonyms: GAD65, Gad-2, 6330404F12Rik, GAD(65)
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14417
HGNC: HGNC:4093
Homologene: 20223
Ptprk
Name: protein tyrosine phosphatase receptor type K
Synonyms: PTPk, RPTPkappa
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 19272
VEGA: 10
HGNC: HGNC:9674
Homologene: 55693
Usp7
Name: ubiquitin specific peptidase 7
Synonyms: 2210010O09Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 252870
Homologene: 2592
Sipa1l1
Name: signal-induced proliferation-associated 1 like 1
Synonyms: 4931426N11Rik, Spar
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 217692
VEGA: 12
Homologene: 9189
Top1
Name: topoisomerase (DNA) I
Synonyms: Top-1, D130064I21Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 21969
Homologene: 2467
Mat2b
Name: methionine adenosyltransferase 2 non-catalytic beta subunit methionine
Synonyms: MATIIbeta, 2410018D16Rik, 1110064C04Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 108645
HGNC: HGNC:6905
Homologene: 8323
Setd2
Name: SET domain containing 2
Synonyms: 4921524K10Rik, KMT3A
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235626
Homologene: 56493
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 20,381,523 bp
  • T to A, chromosome 1 at 128,274,850 bp
  • T to G, chromosome 1 at 153,228,777 bp
  • A to T, chromosome 1 at 162,723,274 bp
  • A to G, chromosome 1 at 174,069,828 bp
  • A to G, chromosome 1 at 179,043,741 bp
  • C to T, chromosome 1 at 191,100,829 bp
  • A to G, chromosome 2 at 10,063,476 bp
  • A to T, chromosome 2 at 22,668,362 bp
  • G to T, chromosome 2 at 30,194,064 bp
  • G to A, chromosome 2 at 68,606,215 bp
  • A to G, chromosome 2 at 76,746,635 bp
  • A to G, chromosome 2 at 76,870,869 bp
  • G to T, chromosome 2 at 112,008,250 bp
  • G to C, chromosome 2 at 119,816,868 bp
  • A to T, chromosome 2 at 121,374,348 bp
  • CTCTAGA to C, chromosome 2 at 145,911,832 bp
  • T to C, chromosome 2 at 160,712,717 bp
  • T to C, chromosome 3 at 87,927,672 bp
  • T to C, chromosome 3 at 96,684,027 bp
  • G to A, chromosome 3 at 108,904,092 bp
  • T to A, chromosome 4 at 11,062,070 bp
  • T to C, chromosome 4 at 94,780,884 bp
  • T to G, chromosome 4 at 111,982,112 bp
  • A to T, chromosome 4 at 118,410,892 bp
  • T to A, chromosome 5 at 18,015,570 bp
  • G to T, chromosome 5 at 36,071,351 bp
  • A to G, chromosome 5 at 67,608,649 bp
  • T to C, chromosome 5 at 75,967,750 bp
  • T to C, chromosome 5 at 124,729,143 bp
  • A to T, chromosome 5 at 136,567,229 bp
  • T to C, chromosome 5 at 145,994,891 bp
  • T to C, chromosome 6 at 4,689,560 bp
  • A to G, chromosome 6 at 18,434,038 bp
  • T to G, chromosome 6 at 37,957,839 bp
  • T to C, chromosome 6 at 118,515,826 bp
  • A to G, chromosome 7 at 18,067,434 bp
  • C to A, chromosome 7 at 24,432,813 bp
  • T to A, chromosome 7 at 46,068,442 bp
  • T to A, chromosome 7 at 79,435,256 bp
  • T to C, chromosome 7 at 80,333,029 bp
  • C to A, chromosome 7 at 90,375,425 bp
  • T to C, chromosome 8 at 117,663,621 bp
  • T to A, chromosome 9 at 19,441,295 bp
  • A to T, chromosome 9 at 79,612,946 bp
  • T to A, chromosome 9 at 86,558,470 bp
  • A to G, chromosome 9 at 90,207,887 bp
  • T to A, chromosome 9 at 100,796,716 bp
  • A to G, chromosome 9 at 110,594,132 bp
  • A to G, chromosome 9 at 118,014,051 bp
  • A to T, chromosome 9 at 122,261,115 bp
  • T to C, chromosome 10 at 28,263,690 bp
  • T to A, chromosome 10 at 29,143,758 bp
  • C to T, chromosome 10 at 59,072,034 bp
  • T to C, chromosome 10 at 129,755,418 bp
  • T to C, chromosome 11 at 26,489,803 bp
  • A to G, chromosome 11 at 40,685,321 bp
  • G to A, chromosome 11 at 43,194,645 bp
  • A to G, chromosome 11 at 51,995,372 bp
  • G to A, chromosome 11 at 59,038,877 bp
  • A to G, chromosome 11 at 60,708,651 bp
  • A to G, chromosome 11 at 75,451,240 bp
  • T to A, chromosome 11 at 100,701,359 bp
  • A to T, chromosome 11 at 116,274,014 bp
  • G to T, chromosome 12 at 71,164,546 bp
  • A to T, chromosome 12 at 71,164,547 bp
  • T to C, chromosome 12 at 72,477,386 bp
  • A to G, chromosome 12 at 75,989,239 bp
  • T to A, chromosome 12 at 82,422,471 bp
  • T to C, chromosome 12 at 86,962,090 bp
  • T to A, chromosome 12 at 101,082,911 bp
  • G to A, chromosome 12 at 101,541,512 bp
  • T to G, chromosome 12 at 112,774,837 bp
  • T to A, chromosome 12 at 116,425,787 bp
  • C to T, chromosome 13 at 22,219,525 bp
  • A to T, chromosome 13 at 23,782,969 bp
  • A to T, chromosome 13 at 34,899,971 bp
  • A to G, chromosome 13 at 74,746,014 bp
  • T to A, chromosome 14 at 52,295,092 bp
  • A to G, chromosome 16 at 3,717,818 bp
  • A to C, chromosome 16 at 8,698,414 bp
  • G to T, chromosome 16 at 34,176,391 bp
  • A to T, chromosome 16 at 45,547,393 bp
  • G to T, chromosome 16 at 58,670,687 bp
  • C to T, chromosome 16 at 91,688,437 bp
  • T to A, chromosome 16 at 92,077,202 bp
  • C to A, chromosome 17 at 24,231,786 bp
  • C to T, chromosome 17 at 27,145,291 bp
  • C to T, chromosome 17 at 32,357,681 bp
  • A to G, chromosome 17 at 34,472,628 bp
  • C to A, chromosome 17 at 34,913,814 bp
  • T to A, chromosome 17 at 34,985,595 bp
  • T to C, chromosome 17 at 35,832,544 bp
  • A to T, chromosome 18 at 34,409,836 bp
  • G to A, chromosome 18 at 36,765,061 bp
  • G to A, chromosome 18 at 60,916,651 bp
  • T to C, chromosome X at 167,352,292 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4651 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041911-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.