Strain Name:
C57BL/6J-MtgxR4653Btlr/Mmmh
Stock Number:
041913-MU
Citation ID:
RRID:MMRRC_041913-MU
Other Names:
R4653 (G1), C57BL/6J-MtgxR4653Btlr
Major Collection:

Strain Information

Pdpk1
Name: 3-phosphoinositide dependent protein kinase 1
Synonyms: Pkb kinase, Pdk1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18607
Homologene: 37643
Gcm2
Name: glial cells missing homolog 2
Synonyms: Gcm1-rs2
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 107889
VEGA: 13
HGNC: HGNC:4198
Homologene: 3490
Gtf3c1
Name: general transcription factor III C 1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233863
HGNC: HGNC:4664
Homologene: 31040
Nomo1
Name: nodal modulator 1
Synonyms: PM5, D7Ertd156e, Nomo
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 211548
Homologene: 13810
Prpf4b
Name: pre-mRNA processing factor 4B
Synonyms: Prpk, Prp4k, Prp4
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 19134
VEGA: 13
Homologene: 134085
R3hdm1
Name: R3H domain containing 1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226412
HGNC: HGNC:9757
Homologene: 9108
Cast
Name: calpastatin
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 12380
VEGA: 13
HGNC: HGNC:1515
Homologene: 7658
Stag1
Name: STAG1 cohesin complex component
Synonyms: Scc3, SA-1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 20842
Homologene: 21191
Git1
Name: GIT ArfGAP 1
Synonyms: p95Cat, Cat-1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216963
HGNC: HGNC:4272
Homologene: 32204
Vps8
Name: VPS8 CORVET complex subunit
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 209018
Homologene: 44592
Lamc1
Name: laminin, gamma 1
Synonyms: Lamb2, laminin B2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226519
HGNC: HGNC:6492
Homologene: 1724
Gcat
Name: glycine C-acetyltransferase (2-amino-3-ketobutyrate-coenzyme A ligase)
Synonyms: aminoacetone synthase, Kbl
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 26912
VEGA: 15
HGNC: HGNC:4188
Homologene: 8475
Sv2a
Name: synaptic vesicle glycoprotein 2a
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 64051
Homologene: 32237
Cnp
Name: 2',3'-cyclic nucleotide 3' phosphodiesterase
Synonyms: Cnp-1, Cnp1, CNPase
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 12799
HGNC: HGNC:2158
Homologene: 7672
Hsd17b13
Name: hydroxysteroid (17-beta) dehydrogenase 13
Synonyms: Pan1b
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 243168
Homologene: 71549
Ube2b
Name: ubiquitin-conjugating enzyme E2B
Synonyms: HR6B, E2-14k, Rad6b
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 22210
Homologene: 101298
Dync1i2
Name: dynein cytoplasmic 1 intermediate chain 2
Synonyms: 3110079H08Rik, Dncic2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 13427
HGNC: HGNC:2964
Homologene: 37921
Ext2
Name: exostosin glycosyltransferase 2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14043
HGNC: HGNC:3513
Homologene: 345
Cul1
Name: cullin 1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 26965
HGNC: HGNC:2551
Homologene: 2663
2610008E11Rik
Name: RIKEN cDNA 2610008E11 gene
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 72128
Homologene: 86705
Dpys
Name: dihydropyrimidinase
Synonyms: 1300004I01Rik, 1200017I10Rik, DHPase
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 64705
HGNC: HGNC:3013
Homologene: 20359
Akr1a1
Name: aldo-keto reductase family 1, member A1
Synonyms: Akr1a4, 2610201A18Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 58810
HGNC: HGNC:380
Homologene: 74565
Fancm
Name: Fanconi anemia, complementation group M
Synonyms: D12Ertd364e, C730036B14Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 104806
VEGA: 12
Homologene: 35378
Cep295
Name: centrosomal protein 295
Synonyms: 5830418K08Rik, LOC382128
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 319675
Homologene: 27936
Ank
Name: progressive ankylosis
Synonyms: D15Ertd221e
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 11732
Homologene: 10664
Mtcl2
Name: microtubule crosslinking factor 2
Synonyms: 9830001H06Rik, Soga1, D430036N24Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 320706
Homologene: 52382
Sppl2a
Name: signal peptide peptidase like 2A
Synonyms: 2010106G01Rik, C130089K23Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 66552
Homologene: 36411
Dnah6
Name: dynein, axonemal, heavy chain 6
Synonyms: A730004I20Rik, Dnahc6
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 330355
HGNC: HGNC:2951
Homologene: 15221
Abca4
Name: ATP-binding cassette, sub-family A member 4
Synonyms: Abc10, Rim protein, RmP, D430003I15Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 11304
HGNC: HGNC:34
Homologene: 298
Cd180
Name: CD180 antigen
Synonyms: RP105, Ly78
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 17079
HGNC: HGNC:6726
Homologene: 4077
Ryr3
Name: ryanodine receptor 3
Synonyms: calcium release channel isoform 3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20192
Homologene: 68151
Abi3
Name: ABI family member 3
Synonyms: 2210414K06Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 66610
Homologene: 9505
Ccdc39
Name: coiled-coil domain containing 39
Synonyms: b2b1735Clo, prh, b2b2025.1Clo, D3Ertd789e, 4921507O14Rik, b2b1304Clo
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 51938
Homologene: 12149
Slc7a14
Name: solute carrier family 7 (cationic amino acid transporter, y+ system), member 14
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 241919
Homologene: 76320
Vmn2r63
Name: vomeronasal 2, receptor 63
Synonyms: EG435975
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 435975
Homologene: 104832
Dpy19l1
Name: dpy-19 like C-mannosyltransferase 1
Synonyms: 1100001I19Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 244745
Homologene: 14762
Trmt1l
Name: tRNA methyltransferase 1 like
Synonyms: Trm1-like, 1190005F20Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 98685
Homologene: 12801
Vmn2r59
Name: vomeronasal 2, receptor 59
Synonyms: EG628444
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 628444
Homologene: 129683
Pde4dip
Name: phosphodiesterase 4D interacting protein (myomegalin)
Synonyms: D130016K21Rik, 9430063L05Rik, Usmg4, 4732458A06Rik, D3Bwg1078e
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 83679
Homologene: 66961
Sspo
Name: SCO-spondin
Synonyms: Scospondin, C79529
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 243369
Homologene: 45453
Folh1
Name: folate hydrolase 1
Synonyms: glutamate carboxypeptidase II, GCP2, mopsm, prostate-specific membrane antigen
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 53320
Homologene: 55826
Myo15b
Name: myosin XVB
Synonyms: LOC217328, E330039G21Rik, LOC380737
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217328
Adhfe1
Name: alcohol dehydrogenase, iron containing, 1
Synonyms: 6330565B14Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 76187
Homologene: 5865
Lrrk1
Name: leucine-rich repeat kinase 1
Synonyms: D130026O16Rik, C230002E15Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233328
Homologene: 23464
Themis2
Name: thymocyte selection associated family member 2
Synonyms: BC013712, ICB-1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230787
Homologene: 21009
Usp13
Name: ubiquitin specific peptidase 13 (isopeptidase T-3)
Synonyms: 2700071E21Rik, ISOT3, IsoT-3
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 72607
Homologene: 68372
3930402G23Rik
Name: RIKEN cDNA 3930402G23 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 665306
Garin5b
Name: golgi associated RAB2 interactor family member 5B
Synonyms: Fam71e2, 4930401F20Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 243822
Homologene: 89225
4933409G03Rik
Name: RIKEN cDNA 4933409G03 gene
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227998
Llgl1
Name: LLGL1 scribble cell polarity complex component
Synonyms: Lgl1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 16897
HGNC: HGNC:6628
Homologene: 31220
Pex26
Name: peroxisomal biogenesis factor 26
Synonyms: peroxisome biogenesis factor 26, 4632428M11Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 74043
Homologene: 9922
Trabd
Name: TraB domain containing
Synonyms: 5730502D15Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 67976
Homologene: 36427
Vmn1r172
Name: vomeronasal 1 receptor 172
Synonyms: V1rd9, V3R9
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 81010
Homologene: 79577
Prpf38b
Name: PRP38 pre-mRNA processing factor 38 (yeast) domain containing B
Synonyms: 1110021E09Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 66921
Homologene: 9986
Trim38
Name: tripartite motif-containing 38
Synonyms: LOC214158
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 214158
Homologene: 48427
Ly9
Name: lymphocyte antigen 9
Synonyms: CD229, SLAMF3, T100, Lgp100
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 17085
HGNC: HGNC:6730
Homologene: 1759
Zbp1
Name: Z-DNA binding protein 1
Synonyms: mZaDLM, Dai, 2010010H03Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 58203
Homologene: 10972
Prps2
Name: phosphoribosyl pyrophosphate synthetase 2
Synonyms: Prps-2, 2610101M19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 110639
HGNC: HGNC:9465
Homologene: 128301
Leprel1
Name:
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Rps6-ps2
Name: ribosomal protein S6, pseudogene 2
Synonyms: Gm16406
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 675985
Rhox2a
Name: reproductive homeobox 2A
Synonyms: Rhox2, 4930539I12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 75199
Homologene: 41772
Gm20496
Name: predicted gene 20496
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 9,550,578 bp
  • T to C, chromosome 1 at 128,184,444 bp
  • G to A, chromosome 1 at 151,439,569 bp
  • T to G, chromosome 1 at 153,228,777 bp
  • G to T, chromosome 1 at 171,594,029 bp
  • G to A, chromosome 2 at 68,606,215 bp
  • A to G, chromosome 2 at 71,247,855 bp
  • T to C, chromosome 2 at 93,696,159 bp
  • T to A, chromosome 2 at 112,652,763 bp
  • C to T, chromosome 2 at 126,920,313 bp
  • A to G, chromosome 2 at 157,040,591 bp
  • G to A, chromosome 2 at 173,207,815 bp
  • A to G, chromosome 3 at 31,257,682 bp
  • A to T, chromosome 3 at 32,837,924 bp
  • A to G, chromosome 3 at 33,819,806 bp
  • T to C, chromosome 3 at 96,190,762 bp
  • A to T, chromosome 3 at 97,767,338 bp
  • G to A, chromosome 3 at 108,904,092 bp
  • G to T, chromosome 3 at 122,138,581 bp
  • A to G, chromosome 4 at 116,637,959 bp
  • A to G, chromosome 4 at 132,782,976 bp
  • G to A, chromosome 5 at 103,965,836 bp
  • T to C, chromosome 6 at 47,484,963 bp
  • T to A, chromosome 6 at 48,478,646 bp
  • T to C, chromosome 6 at 73,073,457 bp
  • C to T, chromosome 6 at 121,190,125 bp
  • T to C, chromosome 7 at 4,758,055 bp
  • G to T, chromosome 7 at 23,660,572 bp
  • A to T, chromosome 7 at 42,043,804 bp
  • A to G, chromosome 7 at 42,903,690 bp
  • G to T, chromosome 7 at 46,061,813 bp
  • T to C, chromosome 7 at 66,273,053 bp
  • C to A, chromosome 7 at 86,744,425 bp
  • T to C, chromosome 7 at 125,674,100 bp
  • A to T, chromosome 8 at 10,926,075 bp
  • A to G, chromosome 8 at 88,806,691 bp
  • T to C, chromosome 9 at 15,335,083 bp
  • A to C, chromosome 9 at 24,482,054 bp
  • T to A, chromosome 9 at 100,796,716 bp
  • T to C, chromosome 10 at 79,067,430 bp
  • A to G, chromosome 11 at 51,995,372 bp
  • A to G, chromosome 11 at 60,708,651 bp
  • A to G, chromosome 11 at 77,505,043 bp
  • T to C, chromosome 11 at 95,832,811 bp
  • A to T, chromosome 11 at 100,576,516 bp
  • G to A, chromosome 11 at 115,879,987 bp
  • A to G, chromosome 12 at 65,083,054 bp
  • A to T, chromosome 13 at 23,782,969 bp
  • A to T, chromosome 13 at 34,899,971 bp
  • A to T, chromosome 13 at 41,102,841 bp
  • A to G, chromosome 13 at 74,746,014 bp
  • T to A, chromosome 13 at 102,704,908 bp
  • T to A, chromosome 15 at 27,590,361 bp
  • C to A, chromosome 15 at 39,793,246 bp
  • T to C, chromosome 15 at 79,035,287 bp
  • A to G, chromosome 15 at 89,085,839 bp
  • T to C, chromosome 16 at 21,500,210 bp
  • T to C, chromosome 16 at 26,105,277 bp
  • A to T, chromosome 17 at 24,106,897 bp
  • C to A, chromosome 17 at 34,200,201 bp
  • G to C, chromosome X at 37,245,309 bp
  • T to C, chromosome X at 167,352,292 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4653 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041913-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.