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Strain Name:
C57BL/6J-MtgxR4659Btlr/Mmmh
Stock Number:
041919-MU
Citation ID:
RRID:MMRRC_041919-MU
Other Names:
R4659 (G1), C57BL/6J-MtgxR4659Btlr
Major Collection:

Strain Information

Limch1
Name: LIM and calponin homology domains 1
Synonyms: 3732412D22Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 77569
Homologene: 18953
Cep70
Name: centrosomal protein 70
Synonyms: 6720484E09Rik, C030018L16Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 68121
Homologene: 11387
Esco2
Name: establishment of sister chromatid cohesion N-acetyltransferase 2
Synonyms: D030072L07Rik, 2410004I17Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 71988
Homologene: 12432
Ranbp17
Name: RAN binding protein 17
Synonyms: 4932704E15Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 66011
Homologene: 36409
Jam2
Name: junction adhesion molecule 2
Synonyms: 2410030G21Rik, JAM-2, VE-JAM, 2410167M24Rik, Jcam2
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 67374
Homologene: 10929
Kcnj1
Name: potassium inwardly-rectifying channel, subfamily J, member 1
Synonyms: ROMK-2, ROMK, Kir1.1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 56379
VEGA: 9
HGNC: HGNC:6255
Homologene: 56764
Cdc42bpb
Name: CDC42 binding protein kinase beta
Synonyms: DMPK-like
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 217866
VEGA: 12
HGNC: HGNC:1738
Homologene: 55945
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 167,377,279 bp
  • G to A, chromosome 2 at 26,319,831 bp
  • T to C, chromosome 2 at 26,470,889 bp
  • A to T, chromosome 2 at 77,212,499 bp
  • G to A, chromosome 2 at 86,255,013 bp
  • T to C, chromosome 2 at 112,479,757 bp
  • A to T, chromosome 2 at 130,770,724 bp
  • A to G, chromosome 2 at 147,061,474 bp
  • A to G, chromosome 2 at 155,826,181 bp
  • T to C, chromosome 3 at 36,025,954 bp
  • T to G, chromosome 3 at 96,559,427 bp
  • T to A, chromosome 3 at 155,129,453 bp
  • T to C, chromosome 4 at 41,521,645 bp
  • A to G, chromosome 4 at 49,530,342 bp
  • T to C, chromosome 4 at 123,322,680 bp
  • A to G, chromosome 4 at 126,948,428 bp
  • A to C, chromosome 5 at 17,476,223 bp
  • T to C, chromosome 5 at 27,497,895 bp
  • T to C, chromosome 5 at 62,654,126 bp
  • C to T, chromosome 5 at 67,027,557 bp
  • T to A, chromosome 5 at 71,641,144 bp
  • T to C, chromosome 5 at 151,062,788 bp
  • T to A, chromosome 6 at 48,484,213 bp
  • A to T, chromosome 6 at 48,906,076 bp
  • A to T, chromosome 6 at 87,019,426 bp
  • A to G, chromosome 6 at 113,414,141 bp
  • C to A, chromosome 6 at 142,672,595 bp
  • A to G, chromosome 7 at 6,393,507 bp
  • A to G, chromosome 7 at 98,085,466 bp
  • A to G, chromosome 7 at 98,710,091 bp
  • T to A, chromosome 8 at 3,510,840 bp
  • C to A, chromosome 8 at 22,259,298 bp
  • A to C, chromosome 8 at 34,849,311 bp
  • T to A, chromosome 8 at 72,695,321 bp
  • C to T, chromosome 8 at 107,391,044 bp
  • T to C, chromosome 8 at 124,897,532 bp
  • C to A, chromosome 9 at 7,453,673 bp
  • G to A, chromosome 9 at 31,399,315 bp
  • A to T, chromosome 9 at 32,394,148 bp
  • T to C, chromosome 9 at 66,527,070 bp
  • T to A, chromosome 9 at 86,502,032 bp
  • T to A, chromosome 9 at 99,296,341 bp
  • T to C, chromosome 9 at 105,560,332 bp
  • A to T, chromosome 9 at 109,744,871 bp
  • G to A, chromosome 10 at 41,486,936 bp
  • T to C, chromosome 10 at 80,803,584 bp
  • T to A, chromosome 11 at 29,578,229 bp
  • T to A, chromosome 11 at 33,266,288 bp
  • A to G, chromosome 12 at 29,849,457 bp
  • T to C, chromosome 12 at 29,994,553 bp
  • T to A, chromosome 12 at 72,470,264 bp
  • T to C, chromosome 12 at 104,413,493 bp
  • T to C, chromosome 12 at 110,628,767 bp
  • T to C, chromosome 12 at 111,339,891 bp
  • T to A, chromosome 13 at 6,553,182 bp
  • G to T, chromosome 14 at 20,367,873 bp
  • G to T, chromosome 14 at 20,564,992 bp
  • G to T, chromosome 14 at 20,683,144 bp
  • G to A, chromosome 14 at 65,826,586 bp
  • G to T, chromosome 15 at 8,216,276 bp
  • A to G, chromosome 15 at 66,673,920 bp
  • A to T, chromosome 15 at 76,242,985 bp
  • G to A, chromosome 16 at 3,866,408 bp
  • C to G, chromosome 16 at 5,212,147 bp
  • T to C, chromosome 16 at 8,664,265 bp
  • G to A, chromosome 16 at 84,812,952 bp
  • G to A, chromosome 16 at 88,562,408 bp
  • T to C, chromosome 16 at 90,776,129 bp
  • T to C, chromosome 16 at 97,455,239 bp
  • G to A, chromosome 17 at 13,517,028 bp
  • A to G, chromosome 17 at 46,725,966 bp
  • T to A, chromosome 18 at 36,969,239 bp
  • A to G, chromosome 18 at 61,974,509 bp
  • C to T, chromosome 19 at 34,125,568 bp
  • T to C, chromosome 19 at 56,576,267 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4659 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041919-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.