Strain Name:
C57BL/6J-MtgxR4819Btlr/Mmmh
Stock Number:
042000-MU
Citation ID:
RRID:MMRRC_042000-MU
Other Names:
R4819 (G1), C57BL/6J-MtgxR4819Btlr
Major Collection:

Gene Information

Gene Symbol: Pax6 [MGI:97490] (Mus musculus (mouse))
Name: paired box 6
Synonyms: Gsfaey11, Dey, Pax-6, 1500038E17Rik, Dickie's small eye, AEY11
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Plin2 [MGI:87920] (Mus musculus (mouse))
Name: perilipin 2
Synonyms: Adrp, adipophilin, ADPH, Adfp
Chromosome: 4
Alteration at locus: Chemically Induced
Gene Symbol: Dst [MGI:104627] (Mus musculus (mouse))
Name: dystonin
Synonyms: ah, 2310001O04Rik, bullous pemphigoid antigen 1, A830042E19Rik, Bpag, Macf2, athetoid, Bpag1, nmf339, nmf203, BPAG1, bullous pemphigoid antigen 1, BPAG1-n
Chromosome: 1
Alteration at locus: Chemically Induced
Gene Symbol: Med1 [MGI:1100846] (Mus musculus (mouse))
Name: mediator complex subunit 1
Synonyms: PBP, TRAP 220, l11Jus15, CRSP210, DRIP205, TRAP220, Pparbp
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Mapk1 [MGI:1346858] (Mus musculus (mouse))
Name: mitogen-activated protein kinase 1
Synonyms: p42mapk, 9030612K14Rik, Erk2, Prkm1, MAPK2
Chromosome: 16
Alteration at locus: Chemically Induced
Gene Symbol: Dctn1 [MGI:107745] (Mus musculus (mouse))
Name: dynactin 1
Synonyms: p150, p150glued, Glued
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Ttc17 [MGI:1921819] (Mus musculus (mouse))
Name: tetratricopeptide repeat domain 17
Synonyms: D2Bwg1005e, 9130020K17Rik
Chromosome: 2
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 33,968,835 bp
  • G to T, chromosome 1 at 119,659,850 bp
  • A to G, chromosome 1 at 136,625,710 bp
  • T to C, chromosome 1 at 169,453,758 bp
  • T to A, chromosome 1 at 170,920,939 bp
  • G to A, chromosome 2 at 25,577,177 bp
  • A to T, chromosome 2 at 76,791,749 bp
  • A to G, chromosome 2 at 82,988,442 bp
  • G to A, chromosome 2 at 94,364,610 bp
  • G to A, chromosome 2 at 105,692,277 bp
  • A to G, chromosome 2 at 109,292,126 bp
  • G to T, chromosome 2 at 129,040,801 bp
  • C to A, chromosome 3 at 5,403,914 bp
  • G to T, chromosome 4 at 55,060,044 bp
  • A to G, chromosome 4 at 86,658,662 bp
  • C to T, chromosome 4 at 107,187,681 bp
  • T to A, chromosome 4 at 116,159,302 bp
  • T to A, chromosome 5 at 30,198,444 bp
  • A to G, chromosome 5 at 111,419,937 bp
  • T to C, chromosome 5 at 120,915,717 bp
  • C to T, chromosome 6 at 4,621,225 bp
  • T to A, chromosome 6 at 24,734,966 bp
  • A to T, chromosome 6 at 31,474,486 bp
  • G to A, chromosome 6 at 83,190,519 bp
  • A to T, chromosome 7 at 24,752,271 bp
  • A to G, chromosome 7 at 24,912,284 bp
  • G to T, chromosome 7 at 109,457,586 bp
  • A to G, chromosome 8 at 9,971,885 bp
  • CGGGGG to CGGGGGG, chromosome 8 at 105,483,744 bp
  • A to T, chromosome 8 at 125,855,230 bp
  • T to A, chromosome 9 at 57,748,397 bp
  • T to A, chromosome 9 at 75,292,202 bp
  • C to T, chromosome 9 at 102,730,071 bp
  • A to G, chromosome 9 at 108,406,678 bp
  • A to G, chromosome 10 at 17,908,072 bp
  • A to C, chromosome 10 at 74,324,389 bp
  • A to G, chromosome 10 at 75,893,879 bp
  • G to T, chromosome 10 at 92,836,477 bp
  • A to G, chromosome 10 at 99,758,082 bp
  • T to A, chromosome 11 at 9,290,421 bp
  • A to T, chromosome 11 at 59,038,848 bp
  • A to G, chromosome 11 at 90,035,445 bp
  • C to T, chromosome 11 at 98,155,432 bp
  • A to G, chromosome 11 at 115,447,943 bp
  • T to A, chromosome 13 at 62,454,417 bp
  • G to A, chromosome 14 at 44,047,060 bp
  • T to A, chromosome 14 at 53,876,613 bp
  • T to C, chromosome 14 at 54,917,153 bp
  • T to C, chromosome 15 at 34,517,982 bp
  • T to A, chromosome 15 at 80,212,349 bp
  • T to C, chromosome 15 at 101,811,544 bp
  • C to T, chromosome 16 at 13,687,467 bp
  • A to G, chromosome 16 at 17,026,495 bp
  • G to A, chromosome 17 at 32,312,305 bp
  • A to G, chromosome 17 at 57,023,360 bp
  • A to G, chromosome 17 at 86,944,538 bp
  • A to G, chromosome 18 at 10,458,358 bp
  • C to T, chromosome 18 at 34,939,388 bp
  • T to A, chromosome 18 at 36,753,257 bp
  • G to T, chromosome 19 at 46,707,529 bp
  • T to A, chromosome 19 at 55,078,341 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4819 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042000-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.