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Strain Name:
C57BL/6J-MtgxR4402Btlr/Mmmh
Stock Number:
042003-MU
Citation ID:
RRID:MMRRC_042003-MU
Other Names:
R4402 (G1), C57BL/6J-MtgxR4402Btlr
Major Collection:

Strain Information

Adamts20
Name: ADAM metallopeptidase with thrombospondin type 1 motif 20
Synonyms: ADAMTS-20, bt
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 223838
Homologene: 11808
Chd7
Name: chromodomain helicase DNA binding protein 7
Synonyms: GENA 60, GENA 47, Gena 52, Cyn, A730019I05Rik, WBE1, Whi, Todo, Obt, Mt, Lda, Flo, Edy, Dz, Cycn
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 320790
Homologene: 19067
Rcan2
Name: regulator of calcineurin 2
Synonyms: MCIP2, ZAKI-4, Csp2, Dscr1l1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 53901
VEGA: 17
HGNC: HGNC:3041
Homologene: 130985
Shc1
Name: src homology 2 domain-containing transforming protein C1
Synonyms: p66, ShcA, p66shc
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 20416
Homologene: 7934
Wwp2
Name: WW domain containing E3 ubiquitin protein ligase 2
Synonyms: 1300010O06Rik, AIP2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 66894
Homologene: 48490
Psmd1
Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 1
Synonyms: P112, S1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 70247
HGNC: HGNC:9554
Homologene: 2100
Rps6kc1
Name: ribosomal protein S6 kinase polypeptide 1
Synonyms: RPK118, B130003F20Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 320119
Homologene: 8244
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 20,239,411 bp
  • A to G, chromosome 1 at 86,075,951 bp
  • CAGAGAGAGAGAGA to CAGAGAGAGAGA, chromosome 1 at 88,211,984 bp
  • C to T, chromosome 1 at 88,215,123 bp
  • G to T, chromosome 1 at 88,254,935 bp
  • C to A, chromosome 1 at 172,102,224 bp
  • A to G, chromosome 1 at 190,798,605 bp
  • C to A, chromosome 2 at 28,451,606 bp
  • A to C, chromosome 2 at 112,249,809 bp
  • T to C, chromosome 3 at 89,426,678 bp
  • T to C, chromosome 3 at 107,986,054 bp
  • T to C, chromosome 3 at 135,336,544 bp
  • T to C, chromosome 3 at 144,952,848 bp
  • A to T, chromosome 4 at 8,866,353 bp
  • T to G, chromosome 4 at 129,742,096 bp
  • T to C, chromosome 4 at 156,225,356 bp
  • C to A, chromosome 5 at 96,642,620 bp
  • T to A, chromosome 5 at 115,470,888 bp
  • T to C, chromosome 5 at 138,178,336 bp
  • A to T, chromosome 6 at 3,708,484 bp
  • T to C, chromosome 6 at 23,969,294 bp
  • T to A, chromosome 6 at 39,166,668 bp
  • C to A, chromosome 6 at 121,879,352 bp
  • C to T, chromosome 6 at 122,315,023 bp
  • T to C, chromosome 6 at 124,456,980 bp
  • T to C, chromosome 7 at 24,377,442 bp
  • T to A, chromosome 7 at 39,221,105 bp
  • T to C, chromosome 7 at 119,834,376 bp
  • C to T, chromosome 8 at 66,484,171 bp
  • G to A, chromosome 8 at 70,290,143 bp
  • A to T, chromosome 8 at 85,525,559 bp
  • A to T, chromosome 8 at 107,418,488 bp
  • T to C, chromosome 8 at 107,457,978 bp
  • T to C, chromosome 10 at 76,392,393 bp
  • C to T, chromosome 10 at 128,043,472 bp
  • T to C, chromosome 11 at 50,304,438 bp
  • T to A, chromosome 11 at 58,880,194 bp
  • T to A, chromosome 11 at 59,503,022 bp
  • A to G, chromosome 11 at 73,589,255 bp
  • A to G, chromosome 11 at 84,819,089 bp
  • A to G, chromosome 12 at 8,989,981 bp
  • A to T, chromosome 12 at 11,242,362 bp
  • T to G, chromosome 12 at 69,959,199 bp
  • T to C, chromosome 12 at 111,449,851 bp
  • A to T, chromosome 12 at 111,541,749 bp
  • T to A, chromosome 13 at 38,023,102 bp
  • C to A, chromosome 13 at 94,418,099 bp
  • T to A, chromosome 13 at 115,001,566 bp
  • A to T, chromosome 14 at 70,408,916 bp
  • T to C, chromosome 15 at 94,379,946 bp
  • G to T, chromosome 16 at 20,678,843 bp
  • A to T, chromosome 16 at 21,976,250 bp
  • C to T, chromosome 16 at 33,989,810 bp
  • T to A, chromosome 17 at 23,176,719 bp
  • A to G, chromosome 17 at 28,219,976 bp
  • A to G, chromosome 17 at 43,953,470 bp
  • A to G, chromosome 18 at 37,295,402 bp
  • A to G, chromosome 18 at 77,441,760 bp
  • G to A, chromosome 18 at 84,871,593 bp
  • T to C, chromosome X at 36,433,493 bp
  • T to C, chromosome X at 93,561,423 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4402 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042003-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.