Strain Name:
C57BL/6J-MtgxR4402Btlr/Mmmh
Stock Number:
042003-MU
Citation ID:
RRID:MMRRC_042003-MU
Other Names:
R4402 (G1), C57BL/6J-MtgxR4402Btlr
Major Collection:

Gene Information

Adamts20
Name: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 20
Synonyms: bt, ADAMTS-20
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 223838
Homologene: 11808
Chd7
Name: chromodomain helicase DNA binding protein 7
Synonyms: Edy, Dz, WBE1, GENA 47, Cycn, Cyn, Obt, Whi, Todo, Gena 52, Lda, Mt, GENA 60, Flo, A730019I05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 320790
Homologene: 19067
Rcan2
Name: regulator of calcineurin 2
Synonyms: Csp2, ZAKI-4, Dscr1l1, MCIP2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 53901
VEGA: 17
HGNC: HGNC:3041
Homologene: 130985
Shc1
Name: src homology 2 domain-containing transforming protein C1
Synonyms: p66, p66shc, ShcA
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 20416
Homologene: 7934
Wwp2
Name: WW domain containing E3 ubiquitin protein ligase 2
Synonyms: AIP2, 1300010O06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 66894
Homologene: 48490
Psmd1
Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 1
Synonyms: S1, P112
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 70247
HGNC: HGNC:9554
Homologene: 2100
Rps6kc1
Name: ribosomal protein S6 kinase polypeptide 1
Synonyms: B130003F20Rik, RPK118
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 320119
Homologene: 8244
Pcnt
Name: pericentrin (kendrin)
Synonyms: m275Asp, m239Asp, Pcnt2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 18541
VEGA: 10
Cyb5a
Name: cytochrome b5 type A (microsomal)
Synonyms: Cyb5, 0610009N12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 109672
HGNC: HGNC:2570
Homologene: 41475
Ap3b1
Name: adaptor-related protein complex 3, beta 1 subunit
Synonyms: rim2, Hps2, recombination induced mutation 2, beta3A, AP-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 11774
VEGA: 13
HGNC: HGNC:566
Homologene: 68125
Pola1
Name: polymerase (DNA directed), alpha 1
Synonyms: Pola
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 18968
HGNC: HGNC:9173
Homologene: 6802
Copa
Name: coatomer protein complex subunit alpha
Synonyms: xenin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12847
HGNC: HGNC:2230
Homologene: 3218
M6pr
Name: mannose-6-phosphate receptor, cation dependent
Synonyms: Mpr46, CD-MPR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 17113
HGNC: HGNC:6752
Homologene: 31086
Eif5
Name: eukaryotic translation initiation factor 5
Synonyms: D12Ertd549e, 2810011H21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 217869
VEGA: 12
HGNC: HGNC:3299
Homologene: 49610
Ap4m1
Name: adaptor-related protein complex AP-4, mu 1
Synonyms: 4930443L05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 11781
HGNC: HGNC:574
Homologene: 3467
Calcr
Name: calcitonin receptor
Synonyms: Clr
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 12311
HGNC: HGNC:1440
Homologene: 1320
Eif4g1
Name: eukaryotic translation initiation factor 4, gamma 1
Synonyms: E030015G23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 208643
HGNC: HGNC:3296
Homologene: 110725
Naca
Name: nascent polypeptide-associated complex alpha polypeptide
Synonyms: LOC380777, skNAC
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 17938
VEGA: 10
HGNC: HGNC:7629
Homologene: 136025
2810021J22Rik
Name: RIKEN cDNA 2810021J22 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 69944
Homologene: 138400
Clca4a
Name: chloride channel accessory 4A
Synonyms: 9130020L07Rik, Clca6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 99663
HGNC: HGNC:2018
Homologene: 40808
Canx
Name: calnexin
Synonyms: 1110069N15Rik, CNX
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 12330
HGNC: HGNC:1473
Homologene: 1324
Khdrbs1
Name: KH domain containing, RNA binding, signal transduction associated 1
Synonyms: p62, Sam68
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 20218
Homologene: 4781
Homer3
Name: homer scaffolding protein 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 26558
Homologene: 37972
Nob1
Name: NIN1/RPN12 binding protein 1 homolog
Synonyms: 1700021I09Rik, Psmd8bp1, Nob1p, ART-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 67619
Homologene: 31924
Kalrn
Name: kalirin, RhoGEF kinase
Synonyms: 2210407G14Rik, E530005C20Rik, LOC224126, Hapip
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 545156
HGNC: HGNC:4814
Homologene: 57160
Atl1
Name: atlastin GTPase 1
Synonyms: AD-FSP, SPG3, FSP1, Spg3a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 73991
VEGA: 12
Homologene: 9302
Zkscan17
Name: zinc finger with KRAB and SCAN domains 17
Synonyms: Zfp496, Nizp1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 268417
Homologene: 13108
Tnfaip2
Name: tumor necrosis factor, alpha-induced protein 2
Synonyms: Exoc3l3, Tnfip2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 21928
Homologene: 4583
Pkhd1
Name: polycystic kidney and hepatic disease 1
Synonyms: FPC, tigmin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 241035
HGNC: HGNC:9016
Homologene: 16336
Loxhd1
Name: lipoxygenase homology domains 1
Synonyms: sba, 1700096C21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 240411
Liph
Name: lipase, member H
Synonyms: Lpdlr, D16Wsu119e, C130037N08Rik, PLA1B, mPA-PLA1, LPDLR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 239759
VEGA: 16
Homologene: 71802
Slc9b2
Name: solute carrier family 9, subfamily B (NHA2, cation proton antiporter 2), member 2
Synonyms: C80638, NHE10, nha-oc, NHA2, Nhedc2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 97086
Homologene: 45381
Kcnn4
Name: potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4
Synonyms: mIKCa1, SK4, IK1, KCa3.1, IKCa1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 16534
HGNC: HGNC:6293
Homologene: 1696
Def6
Name: differentially expressed in FDCP 6
Synonyms: 6430538D02Rik, IRF-4-binding protein, 2410003F05Rik, IBP, SLAT
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 23853
HGNC: HGNC:2760
Homologene: 11103
Gen1
Name: GEN1, Holliday junction 5' flap endonuclease
Synonyms: 5830483C08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 209334
VEGA: 12
Homologene: 35313
Mroh2a
Name: maestro heat-like repeat family member 2A
Synonyms: ENSMUSG00000044873, Heatr7b1, OTTMUSG00000020804
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 100040766
Homologene: 85300
Kdm7a
Name: lysine (K)-specific demethylase 7A
Synonyms: ENSMUSG00000073143, Jhdm1d, A630082K20Rik, Kdm7a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 338523
Homologene: 25281
Fras1
Name: Fraser extracellular matrix complex subunit 1
Synonyms: bl, E130113P14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231470
Homologene: 23516
Clstn3
Name: calsyntenin 3
Synonyms: Cst-3, alcadein-beta, CSTN3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232370
Homologene: 22836
Tma16
Name: translation machinery associated 16
Synonyms: 1810029B16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 66282
Homologene: 10146
Mug1
Name: murinoglobulin 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 17836
Homologene: 136663
Itga1
Name: integrin alpha 1
Synonyms: E130012M19Rik, Vla1, CD49A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 109700
VEGA: 13
HGNC: HGNC:6134
Homologene: 57137
Plekhn1
Name: pleckstrin homology domain containing, family N member 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 231002
VEGA: 4
Homologene: 12949
Pcdhb2
Name: protocadherin beta 2
Synonyms: PcdhbB
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 93873
HGNC: HGNC:8687
Homologene: 69266
Ugt1a6a
Name: UDP glucuronosyltransferase 1 family, polypeptide A6A
Synonyms: UGT1.6, Ugt1a6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 94284
Homologene: 85959
Mrm1
Name: mitochondrial rRNA methyltransferase 1
Synonyms: A530065E19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217038
Homologene: 40307
Tas2r118
Name: taste receptor, type 2, member 118
Synonyms: Tas2r18, mGR18, mt2r40, T2R18
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 387347
Homologene: 9660
Piwil2
Name: piwi-like RNA-mediated gene silencing 2
Synonyms: Miwi like, mili
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 57746
VEGA: 14
Homologene: 23071
Olfr385
Name: olfactory receptor 385
Synonyms: GA_x6K02T2P1NL-3760313-3759375, MOR135-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 259025
Homologene: 133670
Rexo5
Name: RNA exonuclease 5
Synonyms: 2610020H08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 434234
Homologene: 12803
Wdr35
Name: WD repeat domain 35
Synonyms: 4930459M12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 74682
Homologene: 10814
Gstm2
Name: glutathione S-transferase, mu 2
Synonyms: Gstb2, Gstb-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 14863
HGNC: HGNC:4632
Homologene: 121492
Cage1
Name: cancer antigen 1
Synonyms: CAGE1, 4933427I01Rik, Ctag3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 71213
Homologene: 18484
Ppp1r26
Name: protein phosphatase 1, regulatory subunit 26
Synonyms: LOC241289
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 241289
Homologene: 49400
Nutm1
Name: NUT midline carcinoma, family member 1
Synonyms: BC125332, Nut
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 213765
Homologene: 18185
Gpt2
Name: glutamic pyruvate transaminase (alanine aminotransferase) 2
Synonyms: 4631422C05Rik, ALT2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 108682
Homologene: 68832
Zfp40
Name: zinc finger protein 40
Synonyms: NTfin12, Zfp-40
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 22700
Homologene: 136312
Gm14569
Name: predicted gene 14569
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 101055983
Homologene: 141085
Pla2g1b
Name: phospholipase A2, group IB, pancreas
Synonyms: sPLA2IB, Pla2a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 18778
HGNC: HGNC:9030
Homologene: 715
Gm6124
Name: predicted gene 6124
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 620018
Homologene: 45597
Gm20528
Name: predicted gene 20528
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 20,239,411 bp
  • A to G, chromosome 1 at 86,075,951 bp
  • CAGAGAGAGAGAGA to CAGAGAGAGAGA, chromosome 1 at 88,211,984 bp
  • C to T, chromosome 1 at 88,215,123 bp
  • G to T, chromosome 1 at 88,254,935 bp
  • C to A, chromosome 1 at 172,102,224 bp
  • A to G, chromosome 1 at 190,798,605 bp
  • C to A, chromosome 2 at 28,451,606 bp
  • A to C, chromosome 2 at 112,249,809 bp
  • T to C, chromosome 3 at 89,426,678 bp
  • T to C, chromosome 3 at 107,986,054 bp
  • T to C, chromosome 3 at 135,336,544 bp
  • T to C, chromosome 3 at 144,952,848 bp
  • A to T, chromosome 4 at 8,866,353 bp
  • T to G, chromosome 4 at 129,742,096 bp
  • T to C, chromosome 4 at 156,225,356 bp
  • C to A, chromosome 5 at 96,642,620 bp
  • T to A, chromosome 5 at 115,470,888 bp
  • T to C, chromosome 5 at 138,178,336 bp
  • A to T, chromosome 6 at 3,708,484 bp
  • T to C, chromosome 6 at 23,969,294 bp
  • T to A, chromosome 6 at 39,166,668 bp
  • C to A, chromosome 6 at 121,879,352 bp
  • C to T, chromosome 6 at 122,315,023 bp
  • T to C, chromosome 6 at 124,456,980 bp
  • T to C, chromosome 7 at 24,377,442 bp
  • T to A, chromosome 7 at 39,221,105 bp
  • T to C, chromosome 7 at 119,834,376 bp
  • C to T, chromosome 8 at 66,484,171 bp
  • G to A, chromosome 8 at 70,290,143 bp
  • A to T, chromosome 8 at 85,525,559 bp
  • A to T, chromosome 8 at 107,418,488 bp
  • T to C, chromosome 8 at 107,457,978 bp
  • T to C, chromosome 10 at 76,392,393 bp
  • C to T, chromosome 10 at 128,043,472 bp
  • T to C, chromosome 11 at 50,304,438 bp
  • T to A, chromosome 11 at 58,880,194 bp
  • T to A, chromosome 11 at 59,503,022 bp
  • A to G, chromosome 11 at 73,589,255 bp
  • A to G, chromosome 11 at 84,819,089 bp
  • A to G, chromosome 12 at 8,989,981 bp
  • A to T, chromosome 12 at 11,242,362 bp
  • T to G, chromosome 12 at 69,959,199 bp
  • T to C, chromosome 12 at 111,449,851 bp
  • A to T, chromosome 12 at 111,541,749 bp
  • T to A, chromosome 13 at 38,023,102 bp
  • C to A, chromosome 13 at 94,418,099 bp
  • T to A, chromosome 13 at 115,001,566 bp
  • A to T, chromosome 14 at 70,408,916 bp
  • T to C, chromosome 15 at 94,379,946 bp
  • G to T, chromosome 16 at 20,678,843 bp
  • A to T, chromosome 16 at 21,976,250 bp
  • C to T, chromosome 16 at 33,989,810 bp
  • T to A, chromosome 17 at 23,176,719 bp
  • A to G, chromosome 17 at 28,219,976 bp
  • A to G, chromosome 17 at 43,953,470 bp
  • A to G, chromosome 18 at 37,295,402 bp
  • A to G, chromosome 18 at 77,441,760 bp
  • G to A, chromosome 18 at 84,871,593 bp
  • T to C, chromosome X at 36,433,493 bp
  • T to C, chromosome X at 93,561,423 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4402 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042003-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.