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Strain Detail Sheet

Strain Name:
C57BL/6J-MtgxR4732Btlr/Mmmh
Stock Number:
042022-MU
Citation ID:
RRID:MMRRC_042022-MU
Other Names:
R4732 (G1), C57BL/6J-MtgxR4732Btlr
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Qki
Name: quaking, KH domain containing RNA binding
Synonyms: l(17)-1Wis, QkI, l17Wis1, 1110003F05Rik, Qk
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 19317
VEGA: 17
Homologene: 11059

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Tyrp1
Name: tyrosinase-related protein 1
Synonyms: Tyrp, isa, TRP-1, TRP1, Oca3
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 22178
Homologene: 464

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Pank4
Name: pantothenate kinase 4
Synonyms: D030031I12Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 269614
Homologene: 41235

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ClinGen
Sesn2
Name: sestrin 2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230784
Homologene: 12873

Additional Resources
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Gene Ontology
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Fn1
Name: fibronectin 1
Synonyms: Fn-1, Fn
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 14268
HGNC: HGNC:3778
Homologene: 1533

Additional Resources
IMPC
Google
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Gene Ontology
BioGPS
Gene Cloud
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KOMP Phenotyping
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ClinGen
Ptprz1
Name: protein tyrosine phosphatase receptor type Z, polypeptide 1
Synonyms: DSD-1-PG, phosphacan, PTPzeta, PTPbeta, Rptpbeta, Ptpz, Ptprz, RPTPz
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 19283
HGNC: HGNC:9685
Homologene: 2136

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Gpr83
Name: G protein-coupled receptor 83
Synonyms: RP105, RP82, RP39, glucocorticoid-induced receptor, GIR, Gpr72, Gir
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 14608
HGNC: HGNC:4523
Homologene: 7732

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 9,606,976 bp
  • T to A, chromosome 1 at 46,770,173 bp
  • A to T, chromosome 1 at 71,602,512 bp
  • A to T, chromosome 1 at 91,115,647 bp
  • T to G, chromosome 1 at 92,983,385 bp
  • T to C, chromosome 1 at 129,613,186 bp
  • T to C, chromosome 1 at 131,164,727 bp
  • T to A, chromosome 1 at 153,850,741 bp
  • C to T, chromosome 1 at 164,181,657 bp
  • T to A, chromosome 1 at 173,463,876 bp
  • T to C, chromosome 1 at 173,526,591 bp
  • T to A, chromosome 2 at 11,676,920 bp
  • A to T, chromosome 2 at 13,325,381 bp
  • A to G, chromosome 2 at 28,760,071 bp
  • T to A, chromosome 2 at 28,984,388 bp
  • T to G, chromosome 2 at 29,689,160 bp
  • T to C, chromosome 2 at 29,940,232 bp
  • A to G, chromosome 2 at 38,359,991 bp
  • A to G, chromosome 2 at 44,789,055 bp
  • A to G, chromosome 2 at 52,279,079 bp
  • A to G, chromosome 2 at 68,614,721 bp
  • T to C, chromosome 2 at 69,533,555 bp
  • A to G, chromosome 2 at 76,899,827 bp
  • A to T, chromosome 2 at 76,943,011 bp
  • A to T, chromosome 2 at 91,938,339 bp
  • A to G, chromosome 2 at 130,261,397 bp
  • C to A, chromosome 2 at 155,421,301 bp
  • T to A, chromosome 2 at 164,237,123 bp
  • G to T, chromosome 2 at 173,015,392 bp
  • A to G, chromosome 2 at 180,473,615 bp
  • A to G, chromosome 2 at 181,588,739 bp
  • T to C, chromosome 3 at 5,214,807 bp
  • A to T, chromosome 3 at 79,481,652 bp
  • A to T, chromosome 3 at 88,631,940 bp
  • A to T, chromosome 3 at 96,723,661 bp
  • T to A, chromosome 3 at 108,398,952 bp
  • G to T, chromosome 3 at 133,132,269 bp
  • T to C, chromosome 4 at 19,661,990 bp
  • C to T, chromosome 4 at 56,826,404 bp
  • T to C, chromosome 4 at 59,428,029 bp
  • A to T, chromosome 4 at 80,844,935 bp
  • C to A, chromosome 4 at 83,230,491 bp
  • C to A, chromosome 4 at 101,890,710 bp
  • G to A, chromosome 4 at 116,991,673 bp
  • T to C, chromosome 4 at 130,718,193 bp
  • T to C, chromosome 4 at 132,494,591 bp
  • T to A, chromosome 4 at 144,193,941 bp
  • A to G, chromosome 4 at 151,970,967 bp
  • T to A, chromosome 4 at 153,988,426 bp
  • A to T, chromosome 4 at 154,971,390 bp
  • T to A, chromosome 4 at 155,463,602 bp
  • A to G, chromosome 5 at 4,013,901 bp
  • C to T, chromosome 5 at 8,853,491 bp
  • G to T, chromosome 5 at 31,160,057 bp
  • A to G, chromosome 5 at 31,241,066 bp
  • A to G, chromosome 5 at 41,762,144 bp
  • G to A, chromosome 5 at 67,813,120 bp
  • G to T, chromosome 5 at 87,081,538 bp
  • T to C, chromosome 5 at 114,622,753 bp
  • T to A, chromosome 5 at 121,519,434 bp
  • T to A, chromosome 5 at 137,524,770 bp
  • G to A, chromosome 5 at 144,816,570 bp
  • G to A, chromosome 5 at 150,386,007 bp
  • T to A, chromosome 6 at 17,906,516 bp
  • T to A, chromosome 6 at 23,002,610 bp
  • T to G, chromosome 6 at 40,878,859 bp
  • T to A, chromosome 6 at 59,211,415 bp
  • T to A, chromosome 6 at 66,611,819 bp
  • T to C, chromosome 6 at 69,701,000 bp
  • T to A, chromosome 6 at 70,356,296 bp
  • T to A, chromosome 6 at 83,079,957 bp
  • T to C, chromosome 6 at 118,163,193 bp
  • T to C, chromosome 6 at 122,071,872 bp
  • C to A, chromosome 6 at 128,981,879 bp
  • T to A, chromosome 6 at 130,327,132 bp
  • A to G, chromosome 6 at 136,767,152 bp
  • A to G, chromosome 6 at 139,935,985 bp
  • A to T, chromosome 6 at 141,723,179 bp
  • A to G, chromosome 6 at 146,373,173 bp
  • A to C, chromosome 6 at 148,284,980 bp
  • G to A, chromosome 7 at 3,496,900 bp
  • A to T, chromosome 7 at 13,132,327 bp
  • A to T, chromosome 7 at 15,834,772 bp
  • T to A, chromosome 7 at 27,201,043 bp
  • T to A, chromosome 7 at 35,453,563 bp
  • T to C, chromosome 7 at 41,546,749 bp
  • T to A, chromosome 7 at 43,442,189 bp
  • T to C, chromosome 7 at 43,444,200 bp
  • A to G, chromosome 7 at 44,528,885 bp
  • T to A, chromosome 7 at 44,860,454 bp
  • T to C, chromosome 7 at 44,867,109 bp
  • T to C, chromosome 7 at 46,996,110 bp
  • A to G, chromosome 7 at 50,856,217 bp
  • T to C, chromosome 7 at 79,098,609 bp
  • C to T, chromosome 7 at 81,471,932 bp
  • C to T, chromosome 7 at 86,800,987 bp
  • T to C, chromosome 7 at 92,658,833 bp
  • T to C, chromosome 7 at 104,232,648 bp
  • C to A, chromosome 7 at 105,673,849 bp
  • C to A, chromosome 7 at 120,138,120 bp
  • T to C, chromosome 7 at 121,964,244 bp
  • T to A, chromosome 7 at 126,978,358 bp
  • T to C, chromosome 8 at 11,414,779 bp
  • A to G, chromosome 8 at 11,446,197 bp
  • C to T, chromosome 8 at 25,769,488 bp
  • G to A, chromosome 8 at 58,427,813 bp
  • T to C, chromosome 8 at 71,716,795 bp
  • T to A, chromosome 8 at 91,409,714 bp
  • A to G, chromosome 8 at 105,014,604 bp
  • A to G, chromosome 8 at 105,847,136 bp
  • G to A, chromosome 8 at 111,187,755 bp
  • T to A, chromosome 8 at 116,995,842 bp
  • A to G, chromosome 8 at 122,270,274 bp
  • G to T, chromosome 8 at 124,028,897 bp
  • A to T, chromosome 9 at 3,759,424 bp
  • T to A, chromosome 9 at 8,222,173 bp
  • A to G, chromosome 9 at 14,866,174 bp
  • A to T, chromosome 9 at 40,006,268 bp
  • G to A, chromosome 9 at 64,949,554 bp
  • A to G, chromosome 9 at 66,473,516 bp
  • A to T, chromosome 9 at 96,687,684 bp
  • A to C, chromosome 9 at 100,552,502 bp
  • CT to C, chromosome 9 at 104,186,805 bp
  • G to A, chromosome 9 at 105,678,176 bp
  • T to A, chromosome 9 at 110,889,136 bp
  • G to A, chromosome 9 at 121,263,638 bp
  • A to G, chromosome 9 at 121,909,183 bp
  • G to A, chromosome 9 at 123,590,830 bp
  • T to A, chromosome 10 at 3,957,506 bp
  • G to A, chromosome 10 at 42,698,320 bp
  • T to C, chromosome 10 at 43,876,663 bp
  • A to T, chromosome 10 at 83,574,479 bp
  • T to A, chromosome 10 at 117,074,093 bp
  • C to T, chromosome 11 at 8,450,986 bp
  • T to G, chromosome 11 at 29,485,906 bp
  • G to A, chromosome 11 at 50,969,266 bp
  • T to C, chromosome 11 at 60,461,396 bp
  • T to C, chromosome 11 at 61,803,812 bp
  • T to C, chromosome 11 at 73,395,695 bp
  • T to C, chromosome 11 at 76,811,794 bp
  • T to C, chromosome 11 at 77,786,097 bp
  • T to A, chromosome 11 at 83,189,282 bp
  • T to C, chromosome 11 at 94,443,215 bp
  • T to C, chromosome 11 at 113,868,896 bp
  • A to C, chromosome 11 at 116,058,331 bp
  • A to G, chromosome 11 at 116,073,582 bp
  • C to T, chromosome 11 at 120,347,479 bp
  • A to T, chromosome 12 at 51,605,019 bp
  • A to G, chromosome 12 at 81,995,751 bp
  • G to A, chromosome 12 at 104,340,860 bp
  • C to T, chromosome 12 at 110,649,507 bp
  • G to A, chromosome 12 at 112,175,573 bp
  • T to C, chromosome 12 at 114,267,632 bp
  • A to T, chromosome 13 at 11,577,909 bp
  • T to C, chromosome 13 at 24,899,665 bp
  • C to T, chromosome 13 at 54,739,957 bp
  • T to C, chromosome 13 at 65,152,653 bp
  • A to T, chromosome 13 at 102,772,572 bp
  • A to G, chromosome 13 at 113,617,774 bp
  • T to C, chromosome 14 at 26,468,535 bp
  • C to T, chromosome 14 at 26,781,784 bp
  • A to C, chromosome 14 at 31,755,741 bp
  • A to G, chromosome 14 at 32,917,902 bp
  • T to A, chromosome 14 at 50,618,569 bp
  • T to C, chromosome 14 at 54,408,733 bp
  • T to A, chromosome 14 at 55,886,489 bp
  • C to A, chromosome 14 at 69,732,296 bp
  • T to C, chromosome 14 at 79,428,800 bp
  • A to T, chromosome 15 at 11,270,662 bp
  • A to T, chromosome 15 at 36,599,284 bp
  • C to A, chromosome 15 at 55,181,010 bp
  • A to G, chromosome 15 at 64,754,862 bp
  • G to A, chromosome 15 at 78,967,113 bp
  • T to A, chromosome 15 at 82,389,227 bp
  • A to G, chromosome 15 at 89,128,434 bp
  • A to C, chromosome 15 at 91,688,849 bp
  • G to A, chromosome 15 at 91,765,747 bp
  • C to T, chromosome 15 at 99,741,637 bp
  • A to G, chromosome 16 at 10,241,886 bp
  • A to G, chromosome 16 at 17,210,601 bp
  • C to A, chromosome 16 at 62,735,119 bp
  • A to G, chromosome 16 at 81,434,884 bp
  • G to A, chromosome 17 at 7,756,283 bp
  • T to C, chromosome 17 at 10,216,288 bp
  • C to T, chromosome 17 at 21,745,286 bp
  • C to A, chromosome 17 at 24,603,275 bp
  • A to G, chromosome 17 at 27,565,270 bp
  • A to T, chromosome 17 at 28,286,443 bp
  • A to G, chromosome 17 at 30,775,061 bp
  • C to T, chromosome 17 at 33,629,931 bp
  • A to C, chromosome 17 at 37,195,702 bp
  • T to C, chromosome 17 at 37,197,024 bp
  • T to A, chromosome 17 at 38,275,547 bp
  • A to C, chromosome 17 at 42,710,754 bp
  • A to G, chromosome 17 at 46,722,288 bp
  • A to G, chromosome 17 at 46,724,707 bp
  • G to A, chromosome 17 at 53,426,244 bp
  • T to A, chromosome 18 at 45,560,349 bp
  • C to T, chromosome 18 at 63,030,401 bp
  • A to T, chromosome 18 at 68,317,960 bp
  • T to C, chromosome 19 at 4,161,126 bp
  • T to A, chromosome 19 at 6,311,191 bp
  • G to T, chromosome 19 at 9,007,301 bp
  • T to A, chromosome 19 at 10,442,924 bp
  • T to C, chromosome 19 at 31,995,358 bp
  • T to C, chromosome 19 at 34,594,321 bp
  • T to A, chromosome 19 at 40,314,689 bp
  • A to T, chromosome 19 at 44,532,677 bp
  • C to T, chromosome X at 112,372,200 bp
Genotype Determination

Strain Description

Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.

Strain Origin

Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4732 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Black

Breeding

MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
042022-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.