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Strain Name:
C57BL/6J-MtgxR4752Btlr/Mmmh
Stock Number:
042032-MU
Citation ID:
RRID:MMRRC_042032-MU
Other Names:
R4752 (G1), C57BL/6J-MtgxR4752Btlr
Major Collection:

Strain Information

Shprh
Name: SNF2 histone linker PHD RING helicase
Synonyms: 2610103K11Rik, D230017O13Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 268281
Homologene: 6489
Dgka
Name: diacylglycerol kinase, alpha
Synonyms: Dagk1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 13139
VEGA: 10
HGNC: HGNC:2849
Homologene: 1028
Nfib
Name: nuclear factor I/B
Synonyms: 6720429L07Rik, E030026I10Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 18028
HGNC: HGNC:7785
Homologene: 4087
Nisch
Name: nischarin
Synonyms: 1200007D05Rik, 3202002H23Rik, edsn
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 64652
Homologene: 136161
Cep41
Name: centrosomal protein 41
Synonyms: 2810431D15Rik, 1700017E11Rik, Cep41, Tsga14
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 83922
Homologene: 10284
Nomo1
Name: nodal modulator 1
Synonyms: PM5, Nomo, D7Ertd156e
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 211548
Homologene: 13810
Cep170
Name: centrosomal protein 170
Synonyms: 4933426L22Rik, A330004A13Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 545389
Homologene: 22844
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 45,819,622 bp
  • C to T, chromosome 1 at 157,458,649 bp
  • A to T, chromosome 1 at 176,756,688 bp
  • A to G, chromosome 2 at 87,962,349 bp
  • T to A, chromosome 2 at 154,216,280 bp
  • T to A, chromosome 3 at 49,755,114 bp
  • T to C, chromosome 3 at 96,048,387 bp
  • C to A, chromosome 3 at 138,069,990 bp
  • T to A, chromosome 4 at 82,296,779 bp
  • A to T, chromosome 4 at 88,755,488 bp
  • A to G, chromosome 4 at 154,252,438 bp
  • G to A, chromosome 5 at 31,191,231 bp
  • C to T, chromosome 5 at 31,702,047 bp
  • A to G, chromosome 5 at 72,699,151 bp
  • G to A, chromosome 5 at 134,670,441 bp
  • A to G, chromosome 5 at 140,441,101 bp
  • A to G, chromosome 6 at 30,667,441 bp
  • A to T, chromosome 6 at 71,901,312 bp
  • A to G, chromosome 6 at 91,256,243 bp
  • G to T, chromosome 6 at 118,540,465 bp
  • A to T, chromosome 6 at 120,493,034 bp
  • C to T, chromosome 6 at 141,946,614 bp
  • G to A, chromosome 7 at 4,834,250 bp
  • A to G, chromosome 7 at 30,077,881 bp
  • T to A, chromosome 7 at 46,057,202 bp
  • A to G, chromosome 7 at 67,004,470 bp
  • C to T, chromosome 7 at 127,559,000 bp
  • T to C, chromosome 7 at 140,574,602 bp
  • A to T, chromosome 8 at 84,704,281 bp
  • C to T, chromosome 8 at 85,002,720 bp
  • T to C, chromosome 9 at 38,697,970 bp
  • T to A, chromosome 9 at 98,865,647 bp
  • A to G, chromosome 9 at 110,827,768 bp
  • A to G, chromosome 9 at 123,051,386 bp
  • A to T, chromosome 10 at 6,979,573 bp
  • T to C, chromosome 10 at 11,183,803 bp
  • A to T, chromosome 10 at 76,276,657 bp
  • A to T, chromosome 10 at 117,361,368 bp
  • A to T, chromosome 10 at 128,736,659 bp
  • G to A, chromosome 10 at 130,478,467 bp
  • T to C, chromosome 11 at 61,546,371 bp
  • A to G, chromosome 11 at 102,674,433 bp
  • A to G, chromosome 12 at 13,482,537 bp
  • A to G, chromosome 12 at 33,086,103 bp
  • T to A, chromosome 12 at 40,446,365 bp
  • A to T, chromosome 13 at 27,203,525 bp
  • A to T, chromosome 14 at 31,192,588 bp
  • T to A, chromosome 14 at 50,793,665 bp
  • C to T, chromosome 14 at 52,372,307 bp
  • G to A, chromosome 15 at 82,516,371 bp
  • T to C, chromosome 15 at 101,948,202 bp
  • A to G, chromosome 16 at 20,550,576 bp
  • T to C, chromosome 17 at 12,004,123 bp
  • T to A, chromosome 17 at 68,842,240 bp
  • A to G, chromosome 18 at 12,728,725 bp
  • A to T, chromosome 18 at 20,038,222 bp
  • A to G, chromosome 18 at 60,301,081 bp
  • A to G, chromosome 18 at 84,562,914 bp
  • T to A, chromosome 19 at 20,826,649 bp
  • T to C, chromosome 19 at 42,059,904 bp
  • G to T, chromosome 19 at 46,307,567 bp
  • C to T, chromosome X at 74,277,038 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4752 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042032-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.