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Strain Detail Sheet

Strain Name:
B6.Cg-Kittm1.1Sraf/Mmjax
Stock Number:
042035-JAX
Citation ID:
RRID:MMRRC_042035-JAX
Other Names:
c-Kit

Strain Information

Gene Details

Kittm1.1Sraf
Name: KIT proto-oncogene receptor tyrosine kinase; targeted mutation 1.1, Shahin Rafii
Synonyms: c-Kit lox66-71
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Conditional
Kit
Name: KIT proto-oncogene receptor tyrosine kinase
Synonyms: Steel Factor Receptor, c-KIT, Dominant white spotting, belly-spot, Tr-kit, SOW3, SCO5, SCO1, Gsfsow3, Gsfsco5, Gsfsco1, CD117
Type: Gene
Species: Mouse
Chromosome: 5
Alteration at locus: Conditional
NCBI: 16590
HGNC: HGNC:6342
Homologene: 187

Additional Resources
IMPC
Google
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Gene Ontology
BioGPS
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KOMP Phenotyping
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GeneCards
ClinGen
Genetic Alterations
c-Kitlox66-71 is a Cre-recombinase conditional knockout/eGFP-reporter allele - allowing cre-inducible c-Kit deletion and concomitant labeling of the c-Kit-deficient cells with eGFP. These mice are useful for imaging of c-Kit-deficient cells in vivo as well as facilitating isolation of these cells in vitro.
Genotype Determination
ES Cell Line
C2J

Strain Description

Phenotype
c-Kitlox66-71 is a Cre-recombinase conditional knockout/eGFP-reporter allele - allowing cre-inducible c-Kit deletion and concomitant labeling of the c-Kit-deficient cells with eGFP. Prior to Cre recombination, the c-Kitlox66-71 allele will transcribe a full-length c-Kit transcript, and no eGFP expression is reported.

Exposure to Cre recombinase will invert the fragment bordered by the inward-facing lox71 and lox66 sites (lox71-SA-IRES-eGFP-2pA-lox66) into sense orientation; creating the eGFP-expressing c-Kit knockout allele (c-KitΔGFP). Specifically, the transcriptional stops upstream of the exons encoding the transmembrane and kinase domains of c-Kit result in a truncated protein and loss of c-Kit cell surface expression. The splice-acceptor-IRES sequences lead to eGFP expression in c-Kit-expressing cells (direct fluorescence). The donating investigator reports eGFP direct fluorescence is observable by flow cytometry, but weak by imaging. These mice are useful for imaging of c-Kit-deficient cells in vivo as well as facilitating isolation of these cells in vitro.
Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )
MeSH Terms
  • Animals
  • Bone Marrow
  • Cell Adhesion
  • Cell Movement
  • Cell Proliferation
  • Hematopoiesis
  • Hematopoietic Stem Cells
  • Mice
  • Proto-Oncogene Proteins c-kit/physiology
  • Stem Cell Factor
  • Stem Cell Niche
  • Stem Cells/cytology
  • Stem Cells/physiology
Strain Development

The c-Kit lox66-71 allele was designed by Dr. Shahin Rafii (Weill Cornell Medical College). A targeting vector was designed to insert the following, all in reverse transcriptional orientation, into intron 8 of the kit oncogene locus (Kit; c-Kit): frt-flanked PGK-neo-pA cassette, inward-facing lox71 site, splice-acceptor, IRES-eGFP-2pA, inward-facing lox66 site. C57BL/6-Tyrc-2J-derived C2J ES cells. Correctly targeted ES cells were transiently transfected with a Flippase-puromycin plasmid to remove the PGK-neo-pA cassette. Neo-excised ES cells were injected into recipient blastocysts and chimeric males were bred with C57BL/6-Tyrc-2J/J female mice. The donating investigator reports that the c-Kitlox66-71 mice were crossed to C57BL/6; the albino allele (Tyrc-2J)has been bred out.

Suggested Control Mice
Wild-type littermates

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.

Research Applications

  • Cardiovascular
  • Cell Biology
  • Developmental Biology
  • Hematology

Strain Origin

Donor
Shahin Rafii, M.D., Weill Cornell Medicine.
Primary Reference

Kimura Y, Ding B, Nolan DJ, Butler JM, Rafii S. c-Kit-mediated functional positioning of stem cells to their niches is essential for maintenance and regeneration of adult hematopoiesis. PLoS One. 2011;6(10):e26918. (Medline PMID: 22046410)

Colony and Husbandry Information

Health Status Report

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.

Appearance

Coat Color
black

Breeding

MMRRC Breeding System
Random intra-strain mating
Overall Breeding Performance
Good

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Undetermined Undetermined
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
5-9
Recommended wean age
3 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
042035-JAX-SPERM Cryo-preserved spermatozoa $459.00 / Non-Profit Aliquot Approximate quantity3
042035-JAX-RESUS Litter recovered from cryo-archive $2,123.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.