Strain Name:
B6;129S/Sv-Bmp2tm1.1Rog/Mmmh
Stock Number:
042043-MU
Citation ID:
RRID:MMRRC_042043-MU
Other Names:
Bmp2 flU,129S;B6-Bmp2tm1/Mmmh, B6;129Sv-Bmp2tm1.1Rog/Mmmh

Strain Information

Bmp2tm1.1Rog
Name: bone morphogenetic protein 2; targeted mutation 1.1, Melissa Rogers
Synonyms: Bmp2flUCS, Bmp2 flU
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Targeted Mutation
Bmp2
Name: bone morphogenetic protein 2
Synonyms: Bmp2a
Type: Gene
Species: Mouse
Chromosome: 2
Alteration at locus: Targeted Mutation
NCBI: 12156
HGNC: HGNC:1069
Homologene: 926
Genetic Alterations
Flp recombinase-mediated deletion of a frt-flanked Pgk-neo cassette from Bmp2tm1Rog has left a loxP site 76 bp past the translation termination codon and a single frt site followed by a second loxP site 446 base pairs downstream of the stop codon. The loxP sites flank an ultraconserved sequence (UCS) in the 3' untranslated region (3'-UTR) of the mouse gene that mediates post-transcriptional repression of protein synthesis.
Genotype Determination
ES Cell Line
AB2.2 derived from 129S7/SvEvBrd-Hprtb-m2
Phenotype

Homozygous: Mice with are viable and fertile.

Heterozygous: Mice with are viable and fertile.

MeSH Terms
  • 3' Untranslated Regions
  • Animals
  • Bone Morphogenetic Protein 2/genetics
  • Bone Morphogenetic Protein 2/metabolism
  • Conserved Sequence
  • Coronary Vessels/embryology
  • Coronary Vessels/metabolism
  • Gene Deletion
  • Mice
  • Mice, Inbred C57BL
  • Pericardium/embryology
  • Pericardium/metabolism
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
Strain Development
A Bmp2 fragment was isolated from strain 129 and used to construct a 15.9 kb conditional gene targeting vector. The following fragments were subcloned into a bluescript SK vector in order: 1. The upstream homology domain (uHOM) beginning at a SalI site 7313 bp upstream of the translational stop codon to 76 bp past the stop codon followed by a 124 bp loxP site which replaced a mouse specific PvuII site. 2. The Bmp2 UCS from +100 to +446 relative to the stop codon ending at an AccI site. 3. A 1.9 kb Pgk-neo cassette flanked by FRT sites and bearing a downstream loxP site replaced the AccI site. The neo gene conferred kanamycin resistance in E. coli and G418 resistance in mammalian cells. 4. The downstream homology domain beginning at the AccI site at +446 and extending 6197 bp downstream. Linearized targeting vector was electroporated into AB2.2 ES cells (Lexicon Genetics, Woodlands, TX). G418-resistant clones were screened by Southern blot using the indicated external probes flanking the Pgk-neo insertion. Germline targeted mice were generated at the Transgenic/Knock-out core at UT Southwestern Medical Center (UTSW) in Dallas, TX) by injection into C57Bl/6 blastocysts and subsequently screened for agouti coat color. To obtain a pure strain 129 line, chimeras bearing the allele with the Pgk-neo cassette (Bmp2UCSNeo/+) were bred to mice bearing a FLP recombinase gene driven by the constitutive ROSA promoter (129S4/SvJae-Gt(ROSA)26Sortm2(FLP*)Sor/J) to remove the Pgk-neo cassette. This yielded mice with the loxP-flanked UCS (Bmp2flU/+).

The resulting mice were bred to C57Bl/6 once or twice, and then maintained by sib-mating; this mixed strain proved to be more robust.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Apoptosis
  • Cancer
  • Cardiovascular
  • Cell Biology
  • Developmental Biology
  • Internal Organ
  • Research Tools
Donor
Melissa Rogers, Ph.D., Rutgers New Jersey Medical School.
Primary Reference

Shah TA, Zhu Y, Shaikh NN, Harris MA, Harris SE, Rogers MB. Characterization of new bone morphogenetic protein (Bmp)-2 regulatory alleles. Genesis. 2017 Jul;55(7):10.1002/dvg.23035. doi: 10.1002/dvg.23035. Epub 2017 May 22. (Medline PMID: 28401685)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Agouti
MMRRC Breeding System
Backcross: heterozygous x 129S4/SvJaeJ
Generation
none
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Bred to Homozygosity
No
Average litter size
4-6
Recommended wean age
3-4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042043-MU-SPERM Cryo-preserved spermatozoa $437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
042043-MU-RESUS Litter recovered from cryo-archive $2,624.00 / $5,340.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.