Strain Detail Sheet
- Genotyping Protocol(s)
- Center protocol and contact for technical support will be shipped with mice.
Homozygous: VAMP8 null mice developed normally up to birth, but more than one-third were lost before weaning. Growth retardation was observed in the surviving homozygous mice that were roughly 80%–90% of normal sizes before sex maturation. Both male and female surviving mutant mice were fertile.
Heterozygous: No observed phenotype.
Allelic Composition: (Genetic Background: )- renal/urinary system
- colorless urine [MP:0011413] [PMID:19841070] (J:156248)
- decreased urine osmolality [MP:0002988] [PMID:19841070] (J:156248)
- hydronephrosis [MP:0000519] [PMID:19841070] (J:156248)
- kidney atrophy [MP:0004970] [PMID:19841070] (J:156248)
- kidney medulla atrophy [MP:0011422] [PMID:19841070] (J:156248)
- kidney degeneration [MP:0009641] [PMID:19841070] (J:156248)
- behavior/neurological
- increased fluid intake [MP:0011941] [PMID:19841070] (J:156248)
- homeostasis/metabolism
- colorless urine [MP:0011413] [PMID:19841070] (J:156248)
- decreased urine osmolality [MP:0002988] [PMID:19841070] (J:156248)
- Cell Biology
- Diabetes
- Endocrine Deficiency
- Hematology
- Immunology and Inflammation
- Metabolism
- Models for Human Disease
Colony Surveillance Program and Current Health Reports
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.- Wild-type female x Heterozygous male littermate or reciprocal mating
- Heterozygous female x Heterozygous male littermate
- Homozygous female x Homozygous male littermate
Good
Review of Pre-Wean Data: June 26, 2016
A review of the data available concerning birth rate and weaning for this colony (from breeder animals
that produced pups) from late 2014 shows an average litter size of 5.3 and an approximate pre-wean
morbidity rate of 15%.
Meeting Minutes: May 29, 2015
Colony at NYUMC was producing infrequent and small (1-3 offspring) litters compared to breeding colony
at Taconic. Taconic colony (HOM x HOM mating format) was assessed by Genetic and Veterinary
Sciences groups, and found to have a 30% pre-wean morbidity rate. Surviving HOM animals were also
found to be 10-20% smaller than WT, though with normal fecundity and fertility.
Genetic Audit Reports: 2014 and 2016
Genetic audits performed by the Genetics group at Taconic in 2014 and 2016 found that the project in
question was genetically compliant, with no outstanding issues. These genetic audits each included a
review of mendelian distributions and expected results from all mating formats used, as well as a review
of all available mortality data and any abnormalities or adverse phenotypes.
| Viability and Fertility: | Female | Male | Comments |
|---|---|---|---|
| Homozygotes are viable: | Yes | Yes | |
| Homozygotes are fertile: | Yes | Yes | |
| Heterozygotes are fertile: | Yes | Yes | |
| Age Reproductive Decline: | 6 to 8 months | 8 to 12 months |
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
| MMRRC Item # | Description | Distribution Fee / Unit (US $) *Shipping & Handling not included* |
Units | Notes |
|---|---|---|---|---|
| 042052-MU-SPERM | Cryo-preserved spermatozoa | $437.00 / Non-Profit | Aliquot | Approximate quantity3 |
| 042052-MU-RESUS | Litter recovered from cryo-archive | $2,624.00 / Non-Profit | Litter | Recovered litter4; additional fees for any special requests. |
| Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information. | ||||
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.