Strain Name:
B6.129P2-Ctla4tm1Mak/Mmjax
Stock Number:
042058-JAX
Citation ID:
RRID:MMRRC_042058-JAX
Other Names:
Ctla-4,

Strain Information

Ctla4tm1Mak
Name: cytotoxic T-lymphocyte-associated protein 4; targeted mutation 1, Tak W Mak
Synonyms: Ctla-4-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Knockout
Ctla4
Name: cytotoxic T-lymphocyte-associated protein 4
Synonyms: Ly-56, Ctla-4, Cd152
Type: Gene
Species: Mouse
Chromosome: 1
Alteration at locus: Knockout
NCBI: 12477
HGNC: HGNC:2505
Homologene: 3820
Genetic Alterations
The Ctla4 gene (cytotoxic T-lymphocyte-associated protein 4) is disrupted by a Neo inserted in reverse orientation in this mouse. Knockout out mice die by 3-4 weeks of age following an accumulation of T cell blasts, which infiltrate spleen, lymph nodes,liver, heart, lung, bone marrow and pancreas. These mice may be useful for studies of the negative regulation of T cell activation and lymphocyte homeostasis.
ES Cell Line
E14
Phenotype
The Ctla4 (cytotoxic T-lymphocyte-associated protein 4) gene is a member of the immunoglobulin superfamily and encodes a homolog of the E. coli mismatch repair gene mutS. Ctla4 is involved in the regulation of T cell activation. Mice homozygous for the knockout allele are born at the expected Mendelian frequency and are healthy at birth. By 2 weeks of age they sicken and do not live beyond 4 weeks of age. Death is a result of myocardial infarction. Lymph nodes and spleens exhibit an accumulation of T cell blasts as well as an up-regulation of activation markers. Blast cells infiltrate liver, heart, lung, bone marrow and pancreas. Serum immunoglobulin levels of all subtypes are elevated and an expanded population of activated B cells is observed. These mice may be useful for studies of the negative regulation of T cell activation and lymphocyte homeostasis.
Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )

MeSH Terms
  • Abatacept
  • Animals
  • Antigens, CD/analysis
  • Antigens, Differentiation/genetics
  • Antigens, Differentiation/physiology
  • Apoptosis
  • B-Lymphocytes/immunology
  • CTLA-4 Antigen
  • Cells, Cultured
  • Concanavalin A/pharmacology
  • Female
  • Gamma Rays
  • Gene Targeting
  • Homeostasis
  • Immunoconjugates
  • Immunoglobulins/blood
  • Immunophenotyping
  • Lymph Nodes/immunology
  • Lymph Nodes/pathology
  • Lymphocyte Activation
  • Lymphoproliferative Disorders/immunology
  • Lymphoproliferative Disorders/pathology
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Spleen/immunology
  • Spleen/pathology
  • T-Lymphocytes/immunology
  • fas Receptor/metabolism
Strain Development
A targeting vector containing a PGK-neomycin cassette, in reverse orientation, was used to replace exon 2 and a portion of exon 3. The construct was electroporated into 129P2/OlaHsd derived E14K embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric animals were bred to C57BL/6J to achieve germline transmission. The mice were then backcrossed to C57BL/6 for at least 9 generations. Upon arrival, sperm was cryopreserved. To establish a live colony, an aliquot of frozen sperm is used to fertilize C57BL/6J.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.
  • Immunology and Inflammation
Donor
Mak [Ph.D.], Tak
Primary Reference

Waterhouse P, Penninger JM, Timms E, Wakeham A, Shahinian A, Lee KP, Thompson CB, Griesser H, Mak TW. Lymphoproliferative disorders with early lethality in mice deficient in Ctla-4. Science. 1995 Nov 10;270(5238):985-8. (Medline PMID: 7481803)

Colony and Husbandry Information

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.
Coat Color
Black
Other
N/A
MMRRC Breeding System
Backcross
Generation
N9+
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: N/A N/A
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 8 to 12 months 8 to 12 months
Average litter size
4-6
Recommended wean age
3-4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042058-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
042058-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.