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Strain Detail Sheet

Strain Name:
C3H/HeSnJ-Modvl5C3H/HeSnJ/Mmucd
Stock Number:
042070-UCD
Citation ID:
RRID:MMRRC_042070-UCD
Other Names:
Modvl5 (Modifier of vl),

Strain Information

Gene Details

Modvl5C3H/HeSnJ
Name: modifier of vacuolated lens 5; C3H/HeSnJ
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Non-mutant
Modvl5
Name: modifier of vacuolated lens 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Non-mutant
Genetic Alterations
MOLF/EiJ congenic on a C3H/HeSnJ background. The mice are C3H/HeSnJ except at the Modvl5 region, which is MOLF/EiJ.
Genotype Determination
Genotyping Protocol
ES Cell Line
Not applicable

Strain Description

Phenotype

Homozygous: grossly normal

Hetero/Hemizygous: grossly normal

MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Computational Biology
  • Disease Models, Animal
  • Female
  • Frizzled Receptors/genetics
  • Frizzled Receptors/metabolism
  • Frizzled Receptors/physiology
  • Genotype
  • Lens, Crystalline/metabolism
  • Lens, Crystalline/pathology
  • Male
  • Mice
  • Mice, Inbred C3H
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Mutation
  • Neural Tube Defects/genetics
  • Phenotype
  • Polymorphism, Genetic
  • Quantitative Trait Loci/genetics
  • Receptors, Corticotropin/genetics
  • Receptors, Corticotropin/metabolism
  • Receptors, Corticotropin/physiology
  • Receptors, G-Protein-Coupled/genetics
  • Receptors, G-Protein-Coupled/metabolism
  • Receptors, G-Protein-Coupled/physiology
  • Receptors, Melanocortin
  • Sequence Homology, Amino Acid
  • Base Sequence
  • Cell Line
  • Gene Expression Regulation, Developmental
  • Genes, Reporter
  • Genetic Variation
  • Hedgehog Proteins/metabolism
  • Homeodomain Proteins/metabolism
  • In Situ Hybridization
  • Mice, Transgenic
  • Models, Genetic
  • Neurulation
  • Quantitative Trait Loci
  • RNA, Messenger/metabolism
  • Signal Transduction
  • Time Factors
  • Tretinoin/metabolism
  • Wnt Proteins/metabolism
  • Wnt Signaling Pathway
  • Wnt3A Protein/metabolism
Strain Development
To generate the Modvl5 congenics, the C3H/HeSnJ and MOLF/EiJ inbred mice were intercrossed to generate F1 hybrids. C3H/MOLF (C/M) hybrid mice were then backcrossed to isogenic C3H mice for 10 generations. For each generation, genome-wide genotyping was performed by using 65 evenly distributed Simple Sequence Length Polymorphism (SSLP) markers (Whitehead Institute, MIT) that are polymorphic between C3H and MOLF. The male mice with the most contribution to the C3H background but were still C3H/MOLF for the Modvl5 QTL 95% CI (determined by D18MIT50) were selected to generate the next generation. After Modvl5C/M congenics were generated, they were crossed to each other to generate Modvl5M/M.
Suggested Control Mice
Wild-type littermates

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.

Research Applications

  • Developmental Biology
  • Models for Human Disease
  • Neurobiology
  • Research Tools

Strain Origin

Donor
Millonig [Ph.D.], James
Primary Reference

Korstanje R, Desai J, Lazar G, King B, Rollins J, Spurr M, Joseph J, Kadambi S, Li Y, Cherry A, Matteson PG, Paigen B, Millonig JH. Quantitative trait loci affecting phenotypic variation in the vacuolated lens mouse mutant, a multigenic mouse model of neural tube defects. Physiol Genomics. 2008 Nov 12;35(3):296-304. (Medline PMID: 18796533)

Li BI, Matteson PG, Ababon MF, Nato AQ Jr, Lin Y, Nanda V, Matise TC, Millonig JH. The orphan GPCR, Gpr161, regulates the retinoic acid and canonical Wnt pathways during neurulation. Dev Biol. 2015 Jun 1;402(1):17-31. (Medline PMID: 25753732)

Colony and Husbandry Information

Special Considerations

For mating cages, we have found that supplementing with sterile (gamma-irradiated) sunflower seeds greatly improve breeding efficiency.

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Appearance

Coat Color
agouti
Other
N/A

Breeding

MMRRC Breeding System
Sib-mating
Generation
>10 generations
Overall Breeding Performance
Good

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 4 to 6 months 4 to 6 months
Bred to Homozygosity
No
Average litter size
5-9
Recommended wean age
3 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.