Strain Detail Sheet

Strain Name:
C3H.MOLF-Gpr161vl Modvl4MOLF/EiJ/JmilMmucd
Stock Number:
042090-UCD
Citation ID:
RRID:MMRRC_042090-UCD
Other Names:
Modvl4 (Modifier of vacuolated lens 4) Subcongenic1

Strain Information

Gene Details

Modvl4MOLF/EiJ
Name: modifier of vacuolated lens; MOLF/EiJ
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Non-mutant
Modvl4
Name: modifier of vacuolated lens
Type: QTL
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Non-mutant
Gpr161vl
Name: G protein-coupled receptor 161; vacuolated lens
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Spontaneous Mutation
Gpr161
Name: G protein-coupled receptor 161
Synonyms: vl, LOC240888
Type: Gene
Species: Mouse
Chromosome: 1
Alteration at locus: Spontaneous Mutation
NCBI: 240888
Homologene: 17824
Genome Browser [Click image to go to Jbrowse]



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Genetic Alterations
MOLF/EiJ congenic on a C3H/HeSnJ background: the mice are C3H/HeSnJ except at the Modvl4 subcongenic region.
Genotype Determination
Genotyping Protocol

Strain Description

Phenotype

Homozygous: grossly normal

Hetero/Hemizygous: grossly normal

MeSH Terms
  • Animals
  • Base Pairing/genetics
  • Cataract/congenital
  • Cataract/genetics
  • Cell Differentiation
  • Chromosomes, Mammalian/metabolism
  • Crosses, Genetic
  • Female
  • Gene Expression Regulation
  • Genes, Dominant
  • Genetic Association Studies
  • Lens, Crystalline/metabolism
  • Lens, Crystalline/pathology
  • Male
  • Mice
  • Molecular Sequence Annotation
  • Physical Chromosome Mapping
  • Polymorphism, Single Nucleotide/genetics
  • Receptors, G-Protein-Coupled/genetics
  • Receptors, G-Protein-Coupled/metabolism
  • Dentistry
  • Leukoplakia, Oral/diagnosis
  • Mouth Neoplasms/prevention & control
  • Amino Acid Sequence
  • Computational Biology
  • Disease Models, Animal
  • Frizzled Receptors/genetics
  • Frizzled Receptors/metabolism
  • Frizzled Receptors/physiology
  • Genotype
  • Mice, Inbred C3H
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Mutation
  • Neural Tube Defects/genetics
  • Phenotype
  • Polymorphism, Genetic
  • Quantitative Trait Loci/genetics
  • Receptors, Corticotropin/genetics
  • Receptors, Corticotropin/metabolism
  • Receptors, Corticotropin/physiology
  • Receptors, G-Protein-Coupled/physiology
  • Receptors, Melanocortin
  • Sequence Homology, Amino Acid
Strain Development

Compare to MMRRC:42071, which served as a basis for this model.

To generate this line, Gpr161+/vl Modvl4C/M x Gpr161+/vl Modvl4C/C mating was performed (using JAX Strain #000316). Progeny were genotyped for the 14 SSLP markers within the Modvl4 region (see below) as well as for the Gpr161vl mutation. Gpr161+/+ or Gpr161+/vl animals with at least one recombination breakpoint within Modvl4MOLF/EiJ were used as founders to generate subcongenic lines. Fourteen SSLP MIT markers that were evenly distributed within the Modvl4 55-Mb interval (from proximal to distal: D15MIT51, D15MIT81, D15MIT130, D15MIT252, D15MIT267, D15MIT94, D15MIT204, D15MIT49, D15MIT24, D15MIT86, D15MIT59, D15MIT195, D15MIT27, and D15MIT257) were used for genotyping.

Suggested Control Mice
Wild-type littermates

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.

Research Applications

  • Developmental Biology
  • Models for Human Disease
  • Neurobiology
  • Research Tools

Strain Origin

Donor
James Millonig, M.D., Rutgers University.
Primary Reference

Li BI, Ababon MR, Matteson PG, Lin Y, Nanda V, Millonig JH. CongenitalCataract in Gpr161vl/vl Mice Is Modified by Proximal Chromosome 15. PLoS One.2017 Jan 30;12(1):e0170724. doi: 10.1371/journal.pone.0170724. eCollection 2017. (Medline PMID: 28135291)

Bloom HJ. Be reasonable about cancer. Detroit Dent Bull. 1971 Mar;40(3):5. (Medline PMID: 5279759)

Korstanje R, Desai J, Lazar G, King B, Rollins J, Spurr M, Joseph J, Kadambi S, Li Y, Cherry A, Matteson PG, Paigen B, Millonig JH. Quantitative trait loci affecting phenotypic variation in the vacuolated lens mouse mutant, a multigenic mouse model of neural tube defects. Physiol Genomics. 2008 Nov 12;35(3):296-304. doi: 10.1152/physiolgenomics.90260.2008. Epub 2008 Sep 16. (Medline PMID: 18796533)

Colony and Husbandry Information

Special Considerations

For mating cages, we have found that supplementing with sterile (gamma-irradiated) sunflower seeds greatly improve breeding efficiency.

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Appearance

Coat Color

Agouti

Eye
Black

Breeding

MMRRC Breeding System
Backcross and sib-mating
Generation
N10+ (C3H/HeSnJ), F20+
Overall Breeding Performance
Good

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 4 to 6 months 4 to 6 months
Bred to Homozygosity
Yes
Average litter size
5-9
Recommended wean age
3 weeks
Average Pups Weaned
5-9

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.