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Strain Detail Sheet
Strain Name:
C3Sn.Cg-Modvl4MOLF/EiJ/Mmucd
Stock Number:
042091-UCD
Citation ID:
RRID:MMRRC_042091-UCD
Other Names:
Modvl4 (Modifier of vacuolated lens 4) Subcongenic2,
Modvl4MOLF/EiJ
Name: modifier of vacuolated lens; MOLF/EiJ
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Non-mutant
MGI: MGI:5775537
Modvl4
Name: modifier of vacuolated lens
Type: QTL
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Non-mutant
MGI: MGI:3785084
Genetic Alterations
MOLF/EiJ congenic on a C3H/HeSnJ background. The mice are C3H/HeSnJ except at the Modvl4 Subcongenic2 region.
Genotype Determination
Phenotype
Homozygous: grossly normal
Hetero/Hemizygous: grossly normal
Strain Development
To generate the Modvl4MOLF congenics, the C3H/HeSnJ and MOLF/EiJ inbred mice were intercrossed to generate F1 hybrids. C3H/MOLF hybrid mice were then backcrossed to isogenic C3H mice for 10 generations. For each generation, genomewide genotyping was performed by using 65 evenly distributed Simple Sequence Length Polymorphism (SSLP) markers (Whitehead Institute, MIT) that are polymorphic between C3H and MOLF. The male mice with the most contribution to the C3H background but were still C3H/MOLF for the Modvl4 QTL 95% CI were selected to generate the next generation. After Modvl4C/M congenics were generated, they were crossed to each other to generate Modvl4M/M congenics. To generate subcongenic mice, the Gpr161+/vl Modvl4C/M x Gpr161+/vl Modvl4C/C mating was performed. Progeny were genotyped for the 14 SSLP markers within the Modvl4 region detailed below as well as for the Gpr161vl mutation. Gpr161+/+ or Gpr161+/vl animals with at least one recombination breakpoint within Modvl4MOLF were used as founders to generate subcongenic lines. 14 SSLP MIT markers that are evenly distributed within the Modvl4 55 Mb interval (from proximal to distal: D15MIT51, D15MIT81, D15MIT130, D15MIT252, D15MIT267, D15MIT94, D15MIT204, D15MIT49, D15MIT24, D15MIT86, D15MIT59, D15MIT195, D15MIT27 and D15MIT257) were used for genotyping.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
- Developmental Biology
- Models for Human Disease
- Neurobiology
- Research Tools
Donor
James Millonig, Ph.D., Rutgers University.
For mating cages, we have found that supplementing with sterile (gammairradiated) sunflower seeds greatly improve breeding efficiency.
Colony Surveillance Program and Current Health Reports
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.Coat Color
agouti
MMRRC Breeding System
Sib-mating
Generation
>10 generations
Overall Breeding Performance
Good
| Viability and Fertility: | Female | Male | Comments |
|---|---|---|---|
| Homozygotes are viable: | Yes | Yes | |
| Homozygotes are fertile: | Yes | Yes | |
| Heterozygotes are fertile: | Yes | Yes | |
| Age Reproductive Decline: | 4 to 6 months | 4 to 6 months |
Average litter size
5-9
Recommended wean age
3 weeks
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
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- If you register interest in this strain, you will be notified when it becomes available for ordering.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the
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