Strain Name:
C57BL/6-Prkcitm1c(EUCOMM)Hmgu/Mmucd
Stock Number:
042275-UCD
Citation ID:
RRID:MMRRC_042275-UCD
Other Names:
Prkci flox/flox, PKCi flox, C57BL/6-Prkcitm1c(EUCOMM)Hmgu/XxxMmucd

Strain Information

Prkcitm1c(EUCOMM)Hmgu
Name: protein kinase C, iota; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
Synonyms: Prkciflox
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Conditional
Prkci
Name: protein kinase C, iota
Synonyms: Pkcl, Pkci, 2310021H13Rik, Prkcl, aPKClambda, PKClambda
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Conditional
NCBI: 18759
HGNC: HGNC:9404
Homologene: 37667
Genetic Alterations
Flox sequences are inserted around exon 4 of Prkci. In the presence of CRE recombinase, they result in a frame shift followed by an early stop codon, which encodes a nonsense small protein lacking all the regulatory and catalytic domains of PKCi. A vestigial FRT site is located in intron 3 merely 45 bp upstream of the first flox site.
Genotype Determination
ES Cell Line
JM8A3.N1 Derived From C57BL/6N-Atm1Brd
Phenotype

Homozygous: There is no observable phenotype in homozygous animals not crossed with a CRE animal.

Hetero/Hemizygous: We have observed no phenotype in the heterozygous animals.

Cre-excised Phenotype: Undetermined 

MeSH Terms
  • Animals
  • Cell Differentiation/physiology
  • Cell Polarity/physiology
  • Down-Regulation
  • Epithelial Cells/metabolism
  • Female
  • Gene Knockdown Techniques
  • Intestinal Mucosa/cytology
  • Intestinal Mucosa/metabolism
  • Isoenzymes/deficiency
  • Isoenzymes/genetics
  • Isoenzymes/metabolism
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • NF-kappa B/metabolism
  • Protein Kinase C/deficiency
  • Protein Kinase C/genetics
  • Protein Kinase C/metabolism
  • Signal Transduction
Strain Development
ES cells obtained from International Mouse Knockout Consortium (www.mousephenotype.org/; Project 71671). The cells were selected, cloned and genotyped before injection in mouse blastocysts in the C57BL/6 background. Only 1 out of 403 pups from the original chimeric animals showed germline transmission. This animal was crossed with wt C57BL/6, and heterozygous transmission verified by PCR genotyping. These heterozygous animals were crossed with C57BL/6-Tg(CAG-Flpe)2Arte. 7089-M. tg/wt deleters to remove the Neo cassette. Success of these crossings was verified by a new scheme of PCR using both primers for the Neo sequence and for the deleted sequence (longer amplimer due to the residual FLP site and the flox site, as compared with WT). Resulting animals were backcrossed with WT C57BL/6 8 times and then bred to homozygocity.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Cell Biology
  • Diabetes
  • Immunology and Inflammation
  • Models for Human Disease
Donor
Pedro Salas, University of Miami Miller School of Medicine.
Primary Reference

Forteza R, Figueroa Y, Mashukova A, Dulam V, Salas PJ. Conditional knockout of polarity complex (atypical) PKCiota reveals an anti-inflammatory function mediated by NF-kappaB. Mol Biol Cell. 2016 Jul 15;27(14):2186-97. Epub 2016 May 25. (Medline PMID: 27226486)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu .
Coat Color
black
MMRRC Breeding System
Random intra-strain mating
Generation
8
Overall Breeding Performance
Excellent
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 4 to 6 months 6 to 8 months
Average litter size
4-6
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042275-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
042275-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
042275-UCD-RESUS Litter recovered from cryo-archive $3,033.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.