Strain Name:
B6.129S1-Tfe3tm1Est/Mmjax
Stock Number:
042292-JAX
Citation ID:
RRID:MMRRC_042292-JAX
Other Names:
TFE-3 KO,

Gene Information

Tfe3tm1Est
Name: transcription factor E3; targeted mutation 1, Eirikur Steingrimsson
Synonyms: Tfe3Fcr, Tcfe3-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: x
Alteration at locus: Knockout
Tfe3
Name: transcription factor E3
Synonyms: bHLHe33, Tcfe3, F830016E06Rik, Tfe3, Tfe-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Knockout
NCBI: 209446
Homologene: 4755
Genetic Alterations
Knockout of Tfe-3 (Gene ID 209446, MGI:98511)
Genotype Determination
ES Cell Line
CJ7
Phenotype

Homozygous: There is no observable phenotype

Hetero/Hemizygous: None

Cre-excised Phenotype: Undetermined 

Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )

Strain Development
Targeted disruption of the Tfe-3 clones were isolated from a 129/Sv mouse library (Stratagene) by using human Tfe3,The X-chromosome origin (19) of the most strongly hybridizing Tfe3 clone (clone Tfe3-1) was confirmed by interspecific backcross mapping. Similarly, the chromosomal origin of the most strongly hybridizing Tfec clone (clone Tfec4-1) was confirmed by mapping. To generate the Tfe3 targeting vector, two NotI fragments from clone Tfe3-1 were subcloned into pBluescript. A 5.5-kb ClaI-NotI fragment, containing sequences located upstream of the known Tfe3 coding region, was excised and subcloned. Next, a 1.8-kb fragment from the end of the gene was generated by PCR, using primers P1 (5′-gcaacgctccaaagacctggggatccggcagcgg) and P2 (5′-ccagtgccaggactagttggactggcaattccc) and fused with the 5.5-kb clone in the correct orientation. A PgkNeo cassette. The mutant constructs were linearized with NotI and electroporated into CJ7 embryonic stem (ES) cells. DNAs derived from G418/FIAU-resistant ES clones were identified by hybridization. For Tfe3, recombinant clones containing the predicted rearranged bands were obtained at a frequency of 2/179 or 1.1%. For Tfec, a single positive clone was obtained from 300 clones analyzed and its structure confirmed by using an internal probe. The Tfe3 and Tfec mutant clones were injected into C57BL/6J blastocysts, and the resulting chimeras were identified by agouti coat color. Chimeras that transmitted the Tfe3 and Tfec mutations through the germ line were mated to both 129/Sv and C57BL/6J females to establish lines.
Suggested Control Mice
Wild-type littermates
  • Cancer
  • Immunology and Inflammation
  • Models for Human Disease
Donor
Eirikur Steingrimsson, Ph.D., University of Iceland.
Nancy Jenkins, Ph.D., Houston Methodist - Weill Cornell Medical College.
David Fisher, M.D.[Ph.D., Massachusetts General Hospital.

Colony and Husbandry Information

Diet suggestion: Supplement "Love Chow" for parental when female is pregnant; diet Gel H2O for pups when weaned.
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .
Coat Color
Black
MMRRC Breeding System
Sib-mating
Generation
greater than 20
Overall Breeding Performance
Poor. Litters are smaller (runted) and there is increased mortality of pups at 0-3 months.
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: 6 to 8 months 6 to 8 months
Bred to Homozygosity
No
Average litter size
4-6
Recommended wean age
4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042292-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
042292-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.