MMRRC:042412-MU

T to C, chromosome 1 at 67,220,512 bp
C to T, chromosome 1 at 87,620,617 bp
A to T, chromosome 1 at 151,715,694 bp
T to A, chromosome 1 at 159,897,066 bp
T to C, chromosome 1 at 177,937,738 bp
C to A, chromosome 1 at 180,418,194 bp
G to C, chromosome 2 at 40,661,532 bp
A to T, chromosome 2 at 86,298,698 bp
A to C, chromosome 2 at 180,185,941 bp
T to C, chromosome 3 at 69,745,236 bp
C to A, chromosome 3 at 79,465,721 bp
A to T, chromosome 3 at 141,828,517 bp
A to G, chromosome 4 at 19,415,713 bp
T to A, chromosome 4 at 49,450,784 bp
T to C, chromosome 4 at 81,287,711 bp
T to A, chromosome 4 at 125,504,611 bp
C to A, chromosome 4 at 136,610,680 bp
G to A, chromosome 5 at 8,403,062 bp
G to T, chromosome 5 at 92,943,086 bp
A to G, chromosome 5 at 137,310,171 bp
G to A, chromosome 5 at 137,389,019 bp
A to G, chromosome 5 at 140,419,500 bp
T to A, chromosome 6 at 8,427,181 bp
T to A, chromosome 6 at 70,672,285 bp
C to T, chromosome 6 at 71,852,736 bp
G to A, chromosome 6 at 88,910,679 bp
C to T, chromosome 6 at 115,575,933 bp
T to C, chromosome 6 at 142,639,317 bp
T to A, chromosome 7 at 12,600,765 bp
T to A, chromosome 7 at 17,210,535 bp
C to A, chromosome 7 at 44,462,372 bp
T to A, chromosome 7 at 45,948,380 bp
A to T, chromosome 7 at 80,463,848 bp
A to G, chromosome 7 at 89,964,768 bp
GCGGCGGCGGCGGCGGCGGC to GCGGCGGCGGCGGCGGCGGCGGCGGCGGC, chromosome 7 at 97,579,916 bp
A to G, chromosome 7 at 104,012,588 bp
A to G, chromosome 7 at 114,039,867 bp
G to T, chromosome 7 at 116,144,943 bp
G to A, chromosome 7 at 118,121,985 bp
T to A, chromosome 7 at 130,985,026 bp
T to C, chromosome 7 at 139,996,161 bp
T to C, chromosome 8 at 13,577,501 bp
T to C, chromosome 8 at 16,009,369 bp
T to C, chromosome 8 at 56,298,419 bp
T to C, chromosome 8 at 105,990,756 bp
T to G, chromosome 8 at 122,481,112 bp
A to G, chromosome 9 at 38,665,223 bp
G to T, chromosome 9 at 65,390,743 bp
A to T, chromosome 9 at 88,847,652 bp
A to G, chromosome 10 at 42,957,747 bp
G to A, chromosome 10 at 67,224,792 bp
A to C, chromosome 10 at 80,536,322 bp
A to T, chromosome 10 at 128,027,018 bp
T to C, chromosome 11 at 69,163,207 bp
G to A, chromosome 11 at 86,690,007 bp
A to G, chromosome 11 at 105,126,610 bp
G to A, chromosome 11 at 113,657,427 bp
A to T, chromosome 12 at 49,389,907 bp
T to C, chromosome 12 at 73,043,784 bp
T to C, chromosome 12 at 91,234,195 bp
C to T, chromosome 12 at 99,902,364 bp
A to G, chromosome 12 at 111,713,389 bp
A to G, chromosome 13 at 13,740,597 bp
A to G, chromosome 13 at 22,772,476 bp
A to G, chromosome 13 at 55,631,927 bp
A to G, chromosome 13 at 85,250,502 bp
T to A, chromosome 14 at 49,929,269 bp
T to C, chromosome 14 at 51,255,744 bp
T to C, chromosome 15 at 58,577,949 bp
A to C, chromosome 15 at 79,287,618 bp
A to T, chromosome 15 at 98,647,235 bp
C to T, chromosome 15 at 102,108,934 bp
G to T, chromosome 16 at 3,865,987 bp
C to T, chromosome 16 at 17,336,408 bp
T to C, chromosome 16 at 31,455,136 bp
T to G, chromosome 16 at 31,867,909 bp
T to A, chromosome 16 at 35,663,182 bp
A to T, chromosome 16 at 36,781,695 bp
A to G, chromosome 16 at 56,017,159 bp
T to A, chromosome 17 at 23,945,244 bp
T to C, chromosome 17 at 24,644,899 bp
T to A, chromosome 17 at 34,334,401 bp
T to C, chromosome 17 at 35,321,266 bp
T to C, chromosome 17 at 45,633,080 bp
T to C, chromosome 17 at 86,805,758 bp
A to T, chromosome 18 at 21,086,184 bp
T to C, chromosome 18 at 22,912,626 bp
A to G, chromosome 18 at 35,770,445 bp
A to G, chromosome 18 at 64,533,659 bp
T to C, chromosome 19 at 8,895,194 bp
A to G, chromosome 19 at 43,502,906 bp
A to G, chromosome 19 at 48,794,163 bp
C to T, chromosome Y at 916,748 bp

Genotype Determination

Genotyping Protocol(s)
Center protocol and contact for technical support will be shipped with mice.

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.

Strain GQC Summary

Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice

Littermates of all relevant genotypes.

Strain Origin

Donor

Bruce Beutler, M.D., University of Texas Southwestern Medical Center.

Primary Reference

MUTAGENETIX record for R4774 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Strain Development

C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.

Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information Expand All

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color

Black

Breeding

MMRRC Breeding System

Random intra-strain mating

Breeding Scheme(s)

When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.

Overall Breeding Performance

Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size

Undetermined

Recommended wean age

3 weeks

Order Information Expand All

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)

MMRRC Item #	Description	Distribution Fee / Unit (US $) Shipping & Handling not included	Units	Notes
042412-MU-RESUS	Litter recovered from cryo-archive	$5,475.00 / Non-Profit	Litter	Recovered litter⁴; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

¹ The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

² An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

³ An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

⁴ The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.

Strain Detail Sheet

Strain Information Expand All

Colony and Husbandry Information Expand All

Order Information Expand All