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Strain Detail Sheet

Strain Name:
C57BL/6J-MtgxR4825Btlr/Mmmh
Stock Number:
042441-MU
Citation ID:
RRID:MMRRC_042441-MU
Other Names:
R4825 (G1), C57BL/6J-MtgxR4825Btlr
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Nrcam
Name: neuronal cell adhesion molecule
Synonyms: Bravo, C030017F07Rik, C130076O07Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 319504
VEGA: 12
HGNC: HGNC:7994
Homologene: 21041

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Abca12
Name: ATP-binding cassette, sub-family A member 12
Synonyms: 4832428G11Rik, 4833417A11Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 74591
Homologene: 45441

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Tanc1
Name: tetratricopeptide repeat, ankyrin repeat and coiled-coil containing 1
Synonyms: 1200003E16Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 66860
Homologene: 18946

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Rps8
Name: ribosomal protein S8
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 20116
Homologene: 133143

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Mkks
Name: McKusick-Kaufman syndrome
Synonyms: 1300013E18Rik, Bbs6
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 59030
HGNC: HGNC:7108
Homologene: 10318

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IMPC
Google
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Gene Ontology
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Mouse Phenome DB
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Rapgef2
Name: Rap guanine nucleotide exchange factor (GEF) 2
Synonyms: 5830453M24Rik, Pdzgef1, RA-GEF-1, CNRasGEF, nRapGEP
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 76089
Homologene: 35477

Additional Resources
IMPC
Google
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PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
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ClinGen
Slk
Name: STE20-like kinase
Synonyms: 9A2, mSLK, Etk4, SLK, Stk2
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 20874
Homologene: 22515

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 20,537,401 bp
  • T to A, chromosome 1 at 71,302,685 bp
  • T to C, chromosome 1 at 153,903,044 bp
  • A to G, chromosome 1 at 166,491,668 bp
  • A to T, chromosome 1 at 171,605,219 bp
  • A to T, chromosome 1 at 174,244,042 bp
  • A to T, chromosome 1 at 191,340,843 bp
  • A to G, chromosome 2 at 11,587,685 bp
  • A to T, chromosome 2 at 13,325,225 bp
  • A to G, chromosome 2 at 20,806,927 bp
  • C to T, chromosome 2 at 32,371,741 bp
  • A to G, chromosome 2 at 59,699,422 bp
  • T to A, chromosome 2 at 87,372,088 bp
  • T to C, chromosome 2 at 89,248,690 bp
  • A to G, chromosome 2 at 89,439,865 bp
  • G to A, chromosome 2 at 89,978,153 bp
  • A to T, chromosome 2 at 120,055,893 bp
  • A to G, chromosome 2 at 121,542,494 bp
  • A to T, chromosome 2 at 136,880,655 bp
  • G to T, chromosome 2 at 149,899,553 bp
  • G to T, chromosome 2 at 166,585,857 bp
  • T to C, chromosome 3 at 32,992,985 bp
  • T to A, chromosome 3 at 33,801,369 bp
  • C to T, chromosome 3 at 33,813,155 bp
  • T to C, chromosome 3 at 79,083,227 bp
  • G to A, chromosome 3 at 85,673,432 bp
  • T to C, chromosome 3 at 93,652,074 bp
  • T to A, chromosome 3 at 123,114,419 bp
  • T to C, chromosome 3 at 152,217,890 bp
  • T to A, chromosome 3 at 158,061,330 bp
  • C to T, chromosome 3 at 159,624,681 bp
  • T to C, chromosome 4 at 14,546,309 bp
  • T to C, chromosome 4 at 24,536,226 bp
  • A to T, chromosome 4 at 34,571,774 bp
  • G to A, chromosome 4 at 41,520,984 bp
  • T to A, chromosome 4 at 45,912,794 bp
  • G to A, chromosome 4 at 58,850,911 bp
  • A to T, chromosome 4 at 58,957,809 bp
  • C to T, chromosome 4 at 117,124,008 bp
  • G to C, chromosome 4 at 117,155,155 bp
  • G to A, chromosome 4 at 126,791,793 bp
  • T to A, chromosome 4 at 131,799,603 bp
  • A to G, chromosome 4 at 138,194,081 bp
  • G to T, chromosome 5 at 33,672,013 bp
  • C to A, chromosome 5 at 38,159,047 bp
  • A to C, chromosome 5 at 74,588,204 bp
  • G to A, chromosome 5 at 74,588,205 bp
  • T to C, chromosome 5 at 84,233,840 bp
  • T to C, chromosome 5 at 87,250,639 bp
  • T to A, chromosome 5 at 125,783,433 bp
  • C to T, chromosome 5 at 134,404,774 bp
  • T to G, chromosome 5 at 141,582,294 bp
  • T to C, chromosome 6 at 70,315,426 bp
  • A to T, chromosome 6 at 70,766,505 bp
  • A to G, chromosome 6 at 85,678,245 bp
  • G to A, chromosome 6 at 112,727,310 bp
  • A to T, chromosome 6 at 113,498,138 bp
  • G to T, chromosome 6 at 122,713,340 bp
  • A to G, chromosome 6 at 123,815,780 bp
  • T to C, chromosome 7 at 16,908,843 bp
  • T to C, chromosome 7 at 38,402,421 bp
  • T to C, chromosome 7 at 43,692,076 bp
  • C to G, chromosome 7 at 43,783,598 bp
  • T to G, chromosome 7 at 43,845,390 bp
  • T to A, chromosome 7 at 45,624,987 bp
  • C to T, chromosome 7 at 65,647,554 bp
  • T to C, chromosome 7 at 66,849,790 bp
  • T to A, chromosome 7 at 81,865,260 bp
  • T to A, chromosome 7 at 102,542,380 bp
  • T to C, chromosome 7 at 103,937,503 bp
  • G to A, chromosome 7 at 139,026,766 bp
  • T to C, chromosome 7 at 141,868,465 bp
  • A to G, chromosome 8 at 64,752,813 bp
  • G to A, chromosome 8 at 70,839,277 bp
  • T to A, chromosome 8 at 94,941,734 bp
  • T to C, chromosome 8 at 121,917,441 bp
  • T to A, chromosome 8 at 125,135,302 bp
  • A to G, chromosome 9 at 7,581,194 bp
  • A to G, chromosome 9 at 7,654,120 bp
  • T to A, chromosome 9 at 13,431,586 bp
  • T to G, chromosome 9 at 19,783,576 bp
  • A to G, chromosome 9 at 21,955,988 bp
  • A to T, chromosome 9 at 38,756,407 bp
  • A to T, chromosome 9 at 40,006,742 bp
  • A to G, chromosome 9 at 45,068,329 bp
  • G to T, chromosome 9 at 54,404,041 bp
  • T to A, chromosome 9 at 59,624,362 bp
  • T to A, chromosome 9 at 62,454,623 bp
  • A to G, chromosome 9 at 71,630,524 bp
  • A to G, chromosome 9 at 83,988,905 bp
  • T to C, chromosome 9 at 120,017,003 bp
  • T to A, chromosome 10 at 14,131,319 bp
  • T to C, chromosome 10 at 70,283,076 bp
  • A to T, chromosome 10 at 77,941,173 bp
  • A to T, chromosome 10 at 86,947,147 bp
  • T to A, chromosome 10 at 99,009,564 bp
  • T to A, chromosome 10 at 100,488,348 bp
  • T to A, chromosome 10 at 103,418,060 bp
  • A to T, chromosome 10 at 117,818,582 bp
  • T to A, chromosome 11 at 50,308,809 bp
  • A to G, chromosome 11 at 69,423,205 bp
  • G to T, chromosome 11 at 69,685,249 bp
  • T to A, chromosome 11 at 78,208,134 bp
  • T to A, chromosome 11 at 80,132,773 bp
  • T to A, chromosome 11 at 83,758,322 bp
  • C to T, chromosome 11 at 101,468,999 bp
  • C to T, chromosome 11 at 101,738,170 bp
  • C to A, chromosome 11 at 109,791,672 bp
  • C to T, chromosome 11 at 115,243,812 bp
  • A to T, chromosome 11 at 117,869,379 bp
  • G to A, chromosome 11 at 121,195,201 bp
  • C to T, chromosome 12 at 17,289,060 bp
  • C to T, chromosome 12 at 44,575,986 bp
  • T to C, chromosome 12 at 72,077,393 bp
  • T to A, chromosome 12 at 73,932,401 bp
  • A to T, chromosome 12 at 81,502,152 bp
  • A to G, chromosome 12 at 84,126,545 bp
  • A to G, chromosome 12 at 87,200,834 bp
  • G to T, chromosome 13 at 34,630,360 bp
  • T to C, chromosome 13 at 112,988,099 bp
  • T to A, chromosome 14 at 12,220,654 bp
  • G to A, chromosome 14 at 31,077,429 bp
  • A to G, chromosome 14 at 79,214,356 bp
  • G to A, chromosome 15 at 34,442,794 bp
  • T to C, chromosome 15 at 34,999,908 bp
  • A to G, chromosome 15 at 36,597,011 bp
  • C to T, chromosome 15 at 76,120,317 bp
  • G to A, chromosome 15 at 76,633,248 bp
  • T to A, chromosome 15 at 86,351,734 bp
  • C to A, chromosome 16 at 17,574,521 bp
  • C to T, chromosome 16 at 18,784,863 bp
  • A to T, chromosome 16 at 32,751,747 bp
  • G to A, chromosome 17 at 27,877,394 bp
  • G to T, chromosome 17 at 32,418,261 bp
  • C to G, chromosome 17 at 34,906,964 bp
  • G to T, chromosome 17 at 48,351,691 bp
  • A to C, chromosome 17 at 63,863,608 bp
  • A to T, chromosome 17 at 74,047,805 bp
  • T to C, chromosome 18 at 5,114,564 bp
  • T to A, chromosome 18 at 37,520,660 bp
  • T to C, chromosome 18 at 38,189,859 bp
  • A to T, chromosome 18 at 54,897,969 bp
  • A to G, chromosome 18 at 63,144,954 bp
  • A to G, chromosome 19 at 3,614,292 bp
  • A to T, chromosome 19 at 5,108,564 bp
  • A to T, chromosome 19 at 13,314,320 bp
  • T to G, chromosome 19 at 47,619,956 bp
  • C to A, chromosome 19 at 53,370,338 bp
  • A to G, chromosome 19 at 56,473,268 bp
  • C to T, chromosome 19 at 61,226,552 bp
Genotype Determination

Strain Description

Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.

Strain Origin

Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4825 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Black

Breeding

MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
042441-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.