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Strain Name:
C57BL/6J-MtgxR4849Btlr/Mmmh
Stock Number:
042461-MU
Citation ID:
RRID:MMRRC_042461-MU
Other Names:
R4849 (G1), C57BL/6J-MtgxR4849Btlr
Major Collection:

Strain Information

Ing4
Name: inhibitor of growth family, member 4
Synonyms: D6Xrf92, p29ING4, D6Wsu147e
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 28019
Homologene: 22952
Nfia
Name: nuclear factor I/A
Synonyms: 1110047K16Rik, NF1-A, 9430022M17Rik, NF1A
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 18027
HGNC: HGNC:7784
Homologene: 4086
Prkcd
Name: protein kinase C, delta
Synonyms: PKCdelta, PKC[d], Pkcd, D14Ertd420e
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 18753
HGNC: HGNC:9399
Homologene: 55963
App
Name: amyloid beta precursor protein
Synonyms: betaAPP, Abeta, protease nexin II, Adap, Cvap, appican, E030013M08Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 11820
VEGA: 16
HGNC: HGNC:620
Homologene: 56379
Slit1
Name: slit guidance ligand 1
Synonyms: Slil1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 20562
Homologene: 2302
Gaa
Name: glucosidase, alpha, acid
Synonyms: E430018M07Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14387
HGNC: HGNC:4065
Homologene: 37268
Mtmr7
Name: myotubularin related protein 7
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 54384
HGNC: HGNC:7454
Homologene: 99732
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 44,142,873 bp
  • T to C, chromosome 1 at 57,961,993 bp
  • T to G, chromosome 1 at 83,277,384 bp
  • GCC to G, chromosome 1 at 131,275,267 bp
  • T to C, chromosome 1 at 155,130,690 bp
  • C to A, chromosome 1 at 156,857,165 bp
  • T to A, chromosome 1 at 174,369,400 bp
  • A to G, chromosome 1 at 175,620,506 bp
  • C to A, chromosome 2 at 30,011,042 bp
  • T to C, chromosome 2 at 37,146,254 bp
  • T to C, chromosome 2 at 70,244,909 bp
  • T to C, chromosome 2 at 74,659,331 bp
  • T to A, chromosome 2 at 91,615,271 bp
  • T to A, chromosome 2 at 112,908,462 bp
  • C to T, chromosome 2 at 129,609,243 bp
  • T to C, chromosome 2 at 130,532,161 bp
  • C to T, chromosome 2 at 132,534,099 bp
  • T to C, chromosome 2 at 144,585,599 bp
  • T to A, chromosome 2 at 152,949,082 bp
  • T to C, chromosome 2 at 163,865,543 bp
  • T to A, chromosome 2 at 164,832,819 bp
  • A to G, chromosome 3 at 27,459,948 bp
  • A to C, chromosome 4 at 98,081,811 bp
  • A to T, chromosome 4 at 119,367,882 bp
  • A to G, chromosome 4 at 155,840,874 bp
  • T to C, chromosome 5 at 48,224,774 bp
  • T to A, chromosome 5 at 72,302,835 bp
  • T to C, chromosome 5 at 77,306,332 bp
  • T to A, chromosome 5 at 81,766,227 bp
  • A to T, chromosome 5 at 122,591,219 bp
  • A to C, chromosome 5 at 123,759,065 bp
  • A to T, chromosome 5 at 140,693,459 bp
  • A to T, chromosome 6 at 13,196,048 bp
  • A to G, chromosome 6 at 35,230,570 bp
  • A to T, chromosome 6 at 38,630,279 bp
  • T to C, chromosome 6 at 47,859,512 bp
  • T to A, chromosome 6 at 97,116,055 bp
  • T to A, chromosome 6 at 118,532,296 bp
  • A to G, chromosome 6 at 125,043,983 bp
  • A to T, chromosome 6 at 129,056,384 bp
  • A to G, chromosome 7 at 4,122,534 bp
  • A to G, chromosome 7 at 4,643,207 bp
  • G to T, chromosome 7 at 28,615,452 bp
  • A to G, chromosome 7 at 83,935,737 bp
  • A to G, chromosome 7 at 103,098,319 bp
  • G to T, chromosome 7 at 140,202,427 bp
  • A to G, chromosome 8 at 13,373,895 bp
  • A to G, chromosome 8 at 40,608,997 bp
  • A to G, chromosome 8 at 45,012,970 bp
  • A to G, chromosome 8 at 83,662,127 bp
  • G to T, chromosome 8 at 89,187,042 bp
  • A to T, chromosome 8 at 110,726,243 bp
  • T to C, chromosome 9 at 15,322,704 bp
  • A to C, chromosome 9 at 16,377,948 bp
  • A to G, chromosome 9 at 21,811,772 bp
  • T to C, chromosome 9 at 62,797,294 bp
  • T to C, chromosome 9 at 86,792,560 bp
  • A to T, chromosome 9 at 108,056,091 bp
  • T to A, chromosome 9 at 111,025,990 bp
  • T to C, chromosome 10 at 61,104,518 bp
  • T to C, chromosome 10 at 64,873,315 bp
  • A to G, chromosome 10 at 76,294,533 bp
  • G to A, chromosome 10 at 81,592,758 bp
  • A to T, chromosome 10 at 84,375,279 bp
  • T to A, chromosome 11 at 21,148,528 bp
  • T to C, chromosome 11 at 55,310,637 bp
  • C to A, chromosome 11 at 95,076,271 bp
  • A to T, chromosome 11 at 101,317,081 bp
  • G to A, chromosome 11 at 119,272,987 bp
  • T to A, chromosome 12 at 55,698,803 bp
  • A to G, chromosome 12 at 101,934,368 bp
  • A to G, chromosome 12 at 111,781,695 bp
  • A to T, chromosome 13 at 61,201,601 bp
  • G to A, chromosome 13 at 65,152,679 bp
  • A to G, chromosome 14 at 30,599,743 bp
  • G to A, chromosome 16 at 43,871,629 bp
  • T to C, chromosome 16 at 85,056,434 bp
  • T to A, chromosome 17 at 23,972,795 bp
  • T to C, chromosome 17 at 27,032,973 bp
  • T to C, chromosome 17 at 29,347,591 bp
  • T to A, chromosome 17 at 35,987,711 bp
  • T to C, chromosome 17 at 37,405,698 bp
  • T to C, chromosome 17 at 47,394,737 bp
  • T to C, chromosome 17 at 74,647,388 bp
  • C to T, chromosome 17 at 87,983,519 bp
  • C to A, chromosome 18 at 37,755,973 bp
  • A to G, chromosome 19 at 10,918,834 bp
  • T to C, chromosome 19 at 34,594,676 bp
  • G to T, chromosome 19 at 41,649,544 bp
  • A to G, chromosome 19 at 52,264,946 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4849 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042461-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.