Strain Name:
C57BL/6J-MtgxR4891Btlr/Mmmh
Stock Number:
042496-MU
Citation ID:
RRID:MMRRC_042496-MU
Other Names:
R4891 (G1), C57BL/6J-MtgxR4891Btlr
Major Collection:

Strain Information

Edn3
Name: endothelin 3
Synonyms: 114CH19, 114-CH19, tmgc48
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 13616
HGNC: HGNC:3178
Homologene: 88
Rab3gap2
Name: RAB3 GTPase activating protein subunit 2
Synonyms: 1110059F07Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 98732
Homologene: 40842
Zfp462
Name: zinc finger protein 462
Synonyms: Gt4-2, 9430078C22Rik, Zfpip
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242466
Homologene: 41430
Ndufa5
Name: NADH:ubiquinone oxidoreductase subunit A5
Synonyms: 2900002J19Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 68202
HGNC: HGNC:7688
Homologene: 3664
Cluh
Name: clustered mitochondria homolog
Synonyms: 1300001I01Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 74148
Homologene: 9063
Vps13b
Name: vacuolar protein sorting 13B
Synonyms: 1810042B05Rik, Coh1, C330002D13Rik, 2310042E16Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 666173
VEGA: 15
HGNC: HGNC:2183
Homologene: 49516
Sptbn2
Name: spectrin beta, non-erythrocytic 2
Synonyms: Spnb3
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 20743
VEGA: 19
Homologene: 48482
Plxdc2
Name: plexin domain containing 2
Synonyms: 1200007L24Rik, Tem7r, 5430431D22Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 67448
Homologene: 41666
Fam135a
Name: family with sequence similarity 135, member A
Synonyms: 4921533L14Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 68187
Homologene: 32665
Chst1
Name: carbohydrate sulfotransferase 1
Synonyms: KSGAL6ST, C6ST, 2610008E20Rik, GST-1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 76969
HGNC: HGNC:1969
Homologene: 2711
Rnf157
Name: ring finger protein 157
Synonyms: A130073L17Rik, 2610036E23Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217340
Homologene: 28235
Lrp1
Name: low density lipoprotein receptor-related protein 1
Synonyms: CD91, A2mr, b2b1554Clo
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 16971
HGNC: HGNC:6692
Homologene: 1744
Nek10
Name: NIMA (never in mitosis gene a)- related kinase 10
Synonyms: LOC238944
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 674895
Homologene: 130947
Cercam
Name: cerebral endothelial cell adhesion molecule
Synonyms: CerCAM, Ceecam1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 99151
Homologene: 22954
Galns
Name: galactosamine (N-acetyl)-6-sulfatase
Synonyms: N-acetylgalactosamine-6-sulfate sulfatase
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 50917
HGNC: HGNC:4122
Homologene: 55468
Mag
Name: myelin-associated glycoprotein
Synonyms: Gma, siglec-4a
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 17136
HGNC: HGNC:6783
Homologene: 1771
Wdr64
Name: WD repeat domain 64
Synonyms: 4930415O10Rik, 4930511H01Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 75820
Homologene: 51634
Grin2a
Name: glutamate receptor, ionotropic, NMDA2A (epsilon 1)
Synonyms: NR2A, NMDAR2A, GluRepsilon1, GluN2A
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 14811
HGNC: HGNC:4585
Homologene: 645
Kmt2b
Name: lysine (K)-specific methyltransferase 2B
Synonyms: 2610014H22Rik, Mll2, Wbp7
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 75410
Homologene: 22838
Adamts19
Name: ADAM metallopeptidase with thrombospondin type 1 motif 19
Synonyms: D230034E10Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 240322
VEGA: 18
Homologene: 15860
Themis2
Name: thymocyte selection associated family member 2
Synonyms: ICB-1, BC013712
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230787
Homologene: 21009
Thap2
Name: THAP domain containing, apoptosis associated protein 2
Synonyms: 2900040O07Rik, 9030625G08Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 66816
VEGA: 10
Homologene: 12039
Hs3st4
Name: heparan sulfate (glucosamine) 3-O-sulfotransferase 4
Synonyms: Gm6915
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 628779
HGNC: HGNC:5200
Homologene: 82444
Wiz
Name: widely-interspaced zinc finger motifs
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 22404
Homologene: 7997
Zdhhc1
Name: zinc finger, DHHC domain containing 1
Synonyms: 4432412D04Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 70796
Homologene: 36891
Slc5a9
Name: solute carrier family 5 (sodium/glucose cotransporter), member 9
Synonyms: SGLT4
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230612
Homologene: 17081
Stk36
Name: serine/threonine kinase 36
Synonyms: 1700112N14Rik, Fused
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 269209
Homologene: 49432
Slc10a5
Name: solute carrier family 10 (sodium/bile acid cotransporter family), member 5
Synonyms: LOC241877
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 241877
Homologene: 78106
Or13j1
Name: olfactory receptor family 13 subfamily J member 1
Synonyms: mOR17, MOR262-4, GA_x6K02T2N78B-16230286-16231224, Olfr71
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 56015
Homologene: 10460
Vmn1r229
Name: vomeronasal 1 receptor 229
Synonyms: V1re1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 171224
Homologene: 74319
Or5ac24
Name: olfactory receptor family 5 subfamily AC member 24
Synonyms: GA_x54KRFPKG5P-55560552-55559632, MOR182-4, Olfr206
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 258993
Homologene: 74262
Slc16a9
Name: solute carrier family 16 (monocarboxylic acid transporters), member 9
Synonyms: 4930425B13Rik, 1200003C15Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 66859
Homologene: 32642
Gm10729
Name: predicted gene 10729
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Or51g2
Name: olfactory receptor family 51 subfamily G member 2
Synonyms: GA_x6K02T2PBJ9-5685322-5684384, MOR7-2, Olfr577
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 259113
Homologene: 64962
1700034P13Rik
Name: RIKEN cDNA 1700034P13 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 73331
Gm5441
Name: predicted gene 5441
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 432713
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 9,748,062 bp
  • A to G, chromosome 1 at 24,030,328 bp
  • A to G, chromosome 1 at 74,603,256 bp
  • A to G, chromosome 1 at 175,698,779 bp
  • C to T, chromosome 1 at 185,259,366 bp
  • A to G, chromosome 2 at 16,712,146 bp
  • C to A, chromosome 2 at 29,869,271 bp
  • A to G, chromosome 2 at 92,613,992 bp
  • A to G, chromosome 2 at 174,761,732 bp
  • A to T, chromosome 3 at 10,334,625 bp
  • C to T, chromosome 3 at 51,694,510 bp
  • C to T, chromosome 4 at 43,706,194 bp
  • T to C, chromosome 4 at 55,060,055 bp
  • C to A, chromosome 4 at 111,891,744 bp
  • G to T, chromosome 4 at 132,783,357 bp
  • A to G, chromosome 6 at 24,519,247 bp
  • C to T, chromosome 7 at 30,576,761 bp
  • T to C, chromosome 7 at 30,900,368 bp
  • T to A, chromosome 7 at 102,973,552 bp
  • T to A, chromosome 7 at 124,396,829 bp
  • C to T, chromosome 8 at 105,473,017 bp
  • T to A, chromosome 8 at 122,599,156 bp
  • G to A, chromosome 10 at 70,283,956 bp
  • T to C, chromosome 10 at 115,372,696 bp
  • T to C, chromosome 10 at 127,541,752 bp
  • C to T, chromosome 11 at 74,665,059 bp
  • A to G, chromosome 11 at 116,358,670 bp
  • A to G, chromosome 12 at 117,233,365 bp
  • T to A, chromosome 14 at 14,860,986 bp
  • T to A, chromosome 15 at 35,640,515 bp
  • A to G, chromosome 16 at 9,657,706 bp
  • T to A, chromosome 16 at 59,345,471 bp
  • A to T, chromosome 17 at 20,814,819 bp
  • A to G, chromosome 17 at 32,357,628 bp
  • A to G, chromosome 18 at 59,033,000 bp
  • C to T, chromosome 19 at 4,738,469 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4891 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042496-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.