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Strain Name:
C57BL/6J-MtgxR4915Btlr/Mmmh
Stock Number:
042517-MU
Citation ID:
RRID:MMRRC_042517-MU
Other Names:
R4915 (G1), C57BL/6J-MtgxR4915Btlr
Major Collection:

Strain Information

Evl
Name: Ena-vasodilator stimulated phosphoprotein
Synonyms: b2b2600Clo
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 14026
VEGA: 12
Homologene: 56752
Ank2
Name: ankyrin 2, brain
Synonyms: ankyrin B, Ank-2, Ankyrin-2, Ankyrin-B, Gm4392
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 109676
HGNC: HGNC:493
Abca12
Name: ATP-binding cassette, sub-family A member 12
Synonyms: 4832428G11Rik, 4833417A11Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 74591
Homologene: 45441
Scn3a
Name: sodium channel, voltage-gated, type III, alpha
Synonyms: LOC381367, Nav1.3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20269
Homologene: 56005
Nsd1
Name: nuclear receptor-binding SET-domain protein 1
Synonyms: KMT3B
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 18193
VEGA: 13
Homologene: 32543
Ano1
Name: anoctamin 1, calcium activated chloride channel
Synonyms: Tmem16a
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 101772
Homologene: 75079
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 57,902,188 bp
  • T to A, chromosome 1 at 71,349,185 bp
  • A to T, chromosome 1 at 71,595,809 bp
  • T to C, chromosome 1 at 74,147,663 bp
  • A to T, chromosome 1 at 88,055,924 bp
  • T to A, chromosome 1 at 93,074,978 bp
  • T to G, chromosome 1 at 156,844,818 bp
  • A to G, chromosome 1 at 160,441,842 bp
  • G to A, chromosome 2 at 11,490,298 bp
  • G to A, chromosome 2 at 18,682,332 bp
  • A to G, chromosome 2 at 25,298,553 bp
  • A to G, chromosome 2 at 41,788,962 bp
  • T to A, chromosome 2 at 59,914,043 bp
  • T to C, chromosome 2 at 65,461,455 bp
  • A to T, chromosome 2 at 76,811,243 bp
  • T to C, chromosome 2 at 85,754,121 bp
  • A to G, chromosome 2 at 86,554,055 bp
  • T to G, chromosome 2 at 104,054,966 bp
  • A to T, chromosome 2 at 111,754,380 bp
  • A to T, chromosome 2 at 118,790,454 bp
  • A to T, chromosome 2 at 126,720,140 bp
  • C to T, chromosome 2 at 143,827,471 bp
  • T to A, chromosome 2 at 154,314,106 bp
  • T to C, chromosome 2 at 156,031,300 bp
  • T to A, chromosome 2 at 160,982,680 bp
  • G to A, chromosome 2 at 163,607,477 bp
  • T to C, chromosome 2 at 170,378,886 bp
  • T to C, chromosome 2 at 178,449,821 bp
  • T to C, chromosome 2 at 181,232,438 bp
  • C to T, chromosome 3 at 32,585,087 bp
  • A to G, chromosome 3 at 35,997,554 bp
  • T to C, chromosome 3 at 38,107,468 bp
  • A to T, chromosome 3 at 86,824,742 bp
  • T to A, chromosome 3 at 88,101,527 bp
  • T to C, chromosome 3 at 96,019,631 bp
  • C to T, chromosome 3 at 98,450,529 bp
  • T to A, chromosome 3 at 126,942,671 bp
  • C to A, chromosome 4 at 11,230,355 bp
  • A to G, chromosome 4 at 73,731,459 bp
  • A to T, chromosome 4 at 129,210,591 bp
  • T to A, chromosome 4 at 138,873,928 bp
  • C to T, chromosome 5 at 27,500,136 bp
  • C to T, chromosome 5 at 30,864,635 bp
  • T to C, chromosome 5 at 31,476,865 bp
  • G to A, chromosome 5 at 64,100,948 bp
  • T to A, chromosome 5 at 65,803,504 bp
  • A to C, chromosome 5 at 134,725,797 bp
  • C to T, chromosome 5 at 137,583,411 bp
  • A to T, chromosome 5 at 144,805,735 bp
  • A to T, chromosome 5 at 150,478,863 bp
  • A to T, chromosome 6 at 24,566,293 bp
  • T to A, chromosome 6 at 40,585,231 bp
  • A to G, chromosome 6 at 42,675,196 bp
  • T to C, chromosome 6 at 46,530,035 bp
  • T to A, chromosome 6 at 57,510,777 bp
  • T to G, chromosome 6 at 95,126,534 bp
  • T to G, chromosome 7 at 3,330,265 bp
  • C to T, chromosome 7 at 6,961,505 bp
  • T to G, chromosome 7 at 14,562,025 bp
  • T to C, chromosome 7 at 24,177,865 bp
  • C to T, chromosome 7 at 25,080,726 bp
  • T to C, chromosome 7 at 26,384,460 bp
  • C to A, chromosome 7 at 26,937,770 bp
  • T to G, chromosome 7 at 27,878,560 bp
  • T to C, chromosome 7 at 35,752,742 bp
  • T to C, chromosome 7 at 46,044,232 bp
  • C to A, chromosome 7 at 85,621,268 bp
  • A to G, chromosome 7 at 109,901,089 bp
  • T to C, chromosome 7 at 115,476,964 bp
  • T to G, chromosome 7 at 120,670,168 bp
  • T to C, chromosome 7 at 126,444,922 bp
  • A to T, chromosome 7 at 128,685,116 bp
  • A to G, chromosome 7 at 144,611,375 bp
  • C to T, chromosome 8 at 22,561,004 bp
  • T to C, chromosome 8 at 54,602,436 bp
  • T to A, chromosome 8 at 72,009,054 bp
  • T to C, chromosome 8 at 72,468,397 bp
  • T to C, chromosome 8 at 84,771,829 bp
  • T to A, chromosome 8 at 85,110,951 bp
  • T to C, chromosome 9 at 21,111,137 bp
  • G to A, chromosome 9 at 31,108,664 bp
  • T to A, chromosome 9 at 44,612,873 bp
  • C to A, chromosome 9 at 62,752,248 bp
  • A to T, chromosome 9 at 64,947,931 bp
  • C to T, chromosome 9 at 72,385,490 bp
  • A to C, chromosome 9 at 88,715,497 bp
  • A to C, chromosome 9 at 103,251,855 bp
  • A to G, chromosome 9 at 103,254,700 bp
  • G to A, chromosome 9 at 108,966,464 bp
  • A to T, chromosome 9 at 109,274,524 bp
  • A to G, chromosome 9 at 109,383,941 bp
  • T to C, chromosome 10 at 21,395,763 bp
  • T to C, chromosome 10 at 34,009,078 bp
  • C to T, chromosome 10 at 75,925,585 bp
  • A to T, chromosome 10 at 76,715,286 bp
  • T to C, chromosome 10 at 77,901,419 bp
  • A to G, chromosome 10 at 91,149,549 bp
  • T to C, chromosome 10 at 127,199,327 bp
  • A to G, chromosome 10 at 127,850,244 bp
  • C to T, chromosome 11 at 4,214,710 bp
  • T to C, chromosome 11 at 19,009,222 bp
  • T to C, chromosome 11 at 22,146,592 bp
  • A to T, chromosome 11 at 74,121,879 bp
  • T to G, chromosome 11 at 75,656,309 bp
  • A to G, chromosome 11 at 98,420,909 bp
  • T to C, chromosome 11 at 99,387,508 bp
  • C to A, chromosome 11 at 101,076,759 bp
  • A to G, chromosome 11 at 102,352,453 bp
  • G to A, chromosome 11 at 103,910,359 bp
  • T to G, chromosome 11 at 120,816,646 bp
  • T to A, chromosome 12 at 40,069,374 bp
  • C to T, chromosome 12 at 71,189,646 bp
  • G to T, chromosome 12 at 77,475,044 bp
  • C to A, chromosome 12 at 81,974,495 bp
  • T to C, chromosome 12 at 99,719,062 bp
  • A to T, chromosome 12 at 102,837,787 bp
  • G to T, chromosome 12 at 108,686,106 bp
  • C to A, chromosome 12 at 114,434,975 bp
  • T to A, chromosome 13 at 9,621,869 bp
  • A to G, chromosome 13 at 13,499,586 bp
  • G to T, chromosome 13 at 54,738,073 bp
  • T to C, chromosome 13 at 55,247,868 bp
  • A to G, chromosome 13 at 55,276,528 bp
  • A to G, chromosome 13 at 73,938,455 bp
  • T to C, chromosome 13 at 103,752,563 bp
  • T to C, chromosome 13 at 103,752,686 bp
  • A to T, chromosome 14 at 26,734,570 bp
  • T to C, chromosome 14 at 43,234,116 bp
  • A to T, chromosome 14 at 49,232,894 bp
  • A to G, chromosome 14 at 51,990,099 bp
  • T to C, chromosome 14 at 56,553,718 bp
  • A to G, chromosome 14 at 63,048,341 bp
  • A to C, chromosome 15 at 12,162,112 bp
  • T to G, chromosome 15 at 59,321,369 bp
  • G to T, chromosome 15 at 99,720,173 bp
  • A to T, chromosome 16 at 11,199,729 bp
  • C to T, chromosome 16 at 45,980,578 bp
  • A to G, chromosome 17 at 24,909,153 bp
  • A to T, chromosome 17 at 46,714,056 bp
  • A to G, chromosome 17 at 84,186,262 bp
  • T to A, chromosome 18 at 36,968,457 bp
  • G to A, chromosome 19 at 8,968,272 bp
  • A to G, chromosome 19 at 12,241,373 bp
  • T to A, chromosome 19 at 13,145,991 bp
  • C to T, chromosome 19 at 28,763,264 bp
  • T to G, chromosome 19 at 44,971,661 bp
  • A to T, chromosome 19 at 58,267,090 bp
  • C to G, chromosome X at 9,450,750 bp
  • C to T, chromosome Y at 1,316,735 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4915 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042517-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.