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Strain Name:
C57BL/6J-MtgxR4936Btlr/Mmmh
Stock Number:
042536-MU
Citation ID:
RRID:MMRRC_042536-MU
Other Names:
R4936 (G1), C57BL/6J-MtgxR4936Btlr
Major Collection:

Strain Information

Col1a1
Name: collagen, type I, alpha 1
Synonyms: Col1a-1, Mov-13, Cola1, Cola-1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 12842
HGNC: HGNC:2197
Homologene: 73874
Espl1
Name: extra spindle pole bodies 1, separase
Synonyms: separase, SSE, ESP1, PRCE, PRCE, Cerp
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 105988
VEGA: 15
Homologene: 32151
Ank2
Name: ankyrin 2, brain
Synonyms: ankyrin B, Ank-2, Ankyrin-2, Ankyrin-B, Gm4392
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 109676
HGNC: HGNC:493
Gli2
Name: GLI-Kruppel family member GLI2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 14633
HGNC: HGNC:4318
Homologene: 12725
Slc25a12
Name: solute carrier family 25 (mitochondrial carrier, Aralar), member 12
Synonyms: B230107K20Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 78830
Homologene: 48235
Anks1
Name: ankyrin repeat and SAM domain containing 1
Synonyms: Odin
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 224650
Homologene: 9068
Wwp1
Name: WW domain containing E3 ubiquitin protein ligase 1
Synonyms: SDRP1, Tiul1, AIP5, 8030445B08Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 107568
Homologene: 21385
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 74,735,405 bp
  • C to T, chromosome 1 at 87,044,168 bp
  • A to G, chromosome 1 at 118,836,140 bp
  • T to C, chromosome 1 at 129,678,145 bp
  • T to C, chromosome 2 at 27,228,241 bp
  • A to G, chromosome 2 at 32,401,514 bp
  • T to A, chromosome 2 at 67,509,819 bp
  • T to C, chromosome 2 at 71,282,635 bp
  • T to A, chromosome 2 at 82,985,040 bp
  • A to G, chromosome 2 at 87,896,813 bp
  • T to A, chromosome 2 at 93,813,679 bp
  • T to C, chromosome 2 at 115,864,412 bp
  • A to T, chromosome 2 at 148,407,735 bp
  • G to T, chromosome 2 at 181,254,246 bp
  • C to T, chromosome 3 at 30,689,268 bp
  • A to G, chromosome 3 at 109,163,548 bp
  • T to A, chromosome 3 at 126,955,039 bp
  • C to T, chromosome 3 at 135,613,982 bp
  • T to G, chromosome 3 at 154,253,716 bp
  • T to C, chromosome 4 at 19,638,804 bp
  • A to G, chromosome 4 at 43,547,522 bp
  • C to T, chromosome 4 at 63,395,265 bp
  • A to T, chromosome 4 at 87,227,347 bp
  • C to G, chromosome 4 at 132,335,735 bp
  • T to A, chromosome 4 at 139,396,566 bp
  • C to T, chromosome 5 at 31,192,897 bp
  • C to T, chromosome 5 at 34,652,393 bp
  • A to G, chromosome 5 at 43,840,457 bp
  • A to T, chromosome 5 at 75,195,026 bp
  • A to G, chromosome 5 at 96,079,937 bp
  • A to T, chromosome 5 at 98,185,022 bp
  • G to T, chromosome 5 at 98,185,023 bp
  • A to G, chromosome 5 at 116,632,940 bp
  • G to T, chromosome 5 at 140,612,395 bp
  • A to T, chromosome 5 at 142,765,977 bp
  • A to T, chromosome 6 at 47,821,268 bp
  • G to T, chromosome 6 at 48,656,134 bp
  • T to A, chromosome 6 at 87,779,247 bp
  • T to A, chromosome 6 at 91,944,264 bp
  • T to A, chromosome 6 at 106,738,162 bp
  • T to C, chromosome 6 at 117,808,964 bp
  • C to T, chromosome 6 at 125,169,840 bp
  • A to T, chromosome 7 at 16,134,175 bp
  • G to A, chromosome 7 at 30,311,907 bp
  • A to T, chromosome 7 at 57,408,799 bp
  • C to T, chromosome 7 at 122,999,703 bp
  • T to A, chromosome 7 at 126,575,221 bp
  • T to A, chromosome 7 at 127,881,761 bp
  • T to C, chromosome 8 at 24,786,271 bp
  • T to C, chromosome 8 at 79,061,183 bp
  • G to A, chromosome 8 at 105,138,097 bp
  • G to A, chromosome 8 at 114,706,358 bp
  • T to G, chromosome 9 at 38,736,198 bp
  • A to G, chromosome 9 at 80,307,681 bp
  • T to C, chromosome 9 at 108,111,761 bp
  • T to C, chromosome 9 at 108,681,992 bp
  • T to C, chromosome 9 at 109,056,753 bp
  • G to T, chromosome 9 at 110,428,961 bp
  • C to A, chromosome 10 at 14,766,986 bp
  • C to A, chromosome 10 at 63,052,238 bp
  • T to C, chromosome 10 at 68,041,915 bp
  • C to T, chromosome 10 at 81,269,370 bp
  • T to G, chromosome 11 at 69,672,425 bp
  • A to G, chromosome 11 at 87,810,340 bp
  • A to G, chromosome 11 at 94,947,132 bp
  • A to G, chromosome 11 at 116,152,552 bp
  • A to T, chromosome 11 at 120,816,085 bp
  • T to G, chromosome 12 at 80,172,998 bp
  • T to C, chromosome 12 at 83,331,966 bp
  • T to C, chromosome 12 at 111,671,230 bp
  • T to C, chromosome 13 at 21,537,187 bp
  • T to A, chromosome 13 at 33,981,518 bp
  • A to C, chromosome 13 at 34,075,257 bp
  • T to G, chromosome 13 at 49,900,755 bp
  • T to G, chromosome 15 at 11,036,780 bp
  • T to A, chromosome 15 at 39,437,728 bp
  • A to T, chromosome 15 at 102,304,937 bp
  • A to T, chromosome 17 at 18,304,065 bp
  • C to A, chromosome 17 at 27,988,805 bp
  • T to C, chromosome 17 at 28,163,024 bp
  • T to A, chromosome 17 at 35,568,315 bp
  • A to T, chromosome 17 at 50,698,827 bp
  • A to T, chromosome 17 at 55,996,452 bp
  • G to T, chromosome 18 at 37,342,149 bp
  • G to T, chromosome 18 at 37,342,150 bp
  • T to C, chromosome 18 at 50,568,468 bp
  • C to A, chromosome 18 at 80,736,093 bp
  • T to C, chromosome 18 at 84,559,464 bp
  • A to G, chromosome 19 at 23,234,003 bp
  • T to C, chromosome 19 at 30,586,375 bp
  • A to G, chromosome 19 at 38,071,754 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4936 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042536-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.