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Strain Name:
C57BL/6J-MtgxR4946Btlr/Mmmh
Stock Number:
042543-MU
Citation ID:
RRID:MMRRC_042543-MU
Other Names:
R4946 (G1), C57BL/6J-MtgxR4946Btlr
Major Collection:

Strain Information

Myh9
Name: myosin, heavy polypeptide 9, non-muscle
Synonyms: D0Jmb2, E030044M24Rik, NMHC II-A, Myhn-1, Myhn1, myosin IIA, Fltn
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 17886
HGNC: HGNC:7579
Homologene: 129835
Cdk4
Name: cyclin dependent kinase 4
Synonyms: p34PSK-J3/cdk4, Crk3
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 12567
HGNC: HGNC:1773
Homologene: 55429
Ank2
Name: ankyrin 2, brain
Synonyms: ankyrin B, Ank-2, Ankyrin-2, Ankyrin-B, Gm4392
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 109676
HGNC: HGNC:493
Adgre1
Name: adhesion G protein-coupled receptor E1
Synonyms: F4/80, DD7A5-7, TM7LN3, EGF-TM7, Ly71, Emr1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 13733
VEGA: 17
HGNC: HGNC:3336
Homologene: 1493
Lrrn1
Name: leucine rich repeat protein 1, neuronal
Synonyms: NLRR-1, 2810047E21Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 16979
Homologene: 32036
Psmb1
Name: proteasome (prosome, macropain) subunit, beta type 1
Synonyms: Lmpc5
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 19170
VEGA: 17
HGNC: HGNC:9537
Homologene: 2087
Pcnt
Name: pericentrin (kendrin)
Synonyms: Pcnt2, m275Asp, m239Asp
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 18541
VEGA: 10
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to G, chromosome 1 at 174,050,834 bp
  • C to T, chromosome 2 at 14,045,137 bp
  • A to G, chromosome 2 at 24,925,848 bp
  • T to C, chromosome 2 at 65,150,969 bp
  • C to A, chromosome 2 at 68,118,569 bp
  • T to C, chromosome 2 at 76,752,426 bp
  • C to A, chromosome 2 at 76,918,709 bp
  • T to A, chromosome 2 at 80,658,399 bp
  • A to G, chromosome 2 at 83,788,983 bp
  • A to G, chromosome 2 at 97,630,489 bp
  • T to C, chromosome 2 at 111,483,966 bp
  • A to G, chromosome 2 at 135,345,095 bp
  • T to C, chromosome 2 at 154,368,874 bp
  • T to A, chromosome 2 at 158,440,967 bp
  • A to T, chromosome 3 at 45,379,482 bp
  • T to C, chromosome 3 at 54,194,985 bp
  • A to G, chromosome 3 at 108,980,228 bp
  • A to T, chromosome 3 at 126,941,940 bp
  • T to C, chromosome 3 at 158,005,773 bp
  • A to T, chromosome 4 at 9,281,831 bp
  • T to A, chromosome 4 at 33,231,174 bp
  • A to G, chromosome 4 at 119,183,308 bp
  • A to T, chromosome 5 at 30,144,162 bp
  • A to G, chromosome 5 at 72,604,764 bp
  • G to A, chromosome 5 at 107,177,913 bp
  • A to G, chromosome 5 at 110,253,838 bp
  • T to C, chromosome 6 at 57,432,174 bp
  • A to G, chromosome 6 at 107,568,890 bp
  • A to G, chromosome 6 at 115,451,028 bp
  • T to A, chromosome 7 at 18,856,683 bp
  • C to A, chromosome 7 at 30,958,209 bp
  • G to T, chromosome 7 at 42,430,155 bp
  • A to T, chromosome 7 at 72,259,269 bp
  • T to A, chromosome 7 at 104,022,012 bp
  • A to G, chromosome 7 at 107,815,382 bp
  • T to A, chromosome 7 at 119,931,560 bp
  • G to T, chromosome 7 at 126,396,612 bp
  • A to T, chromosome 8 at 4,301,752 bp
  • T to C, chromosome 8 at 33,742,559 bp
  • T to C, chromosome 8 at 69,903,064 bp
  • T to G, chromosome 8 at 105,381,996 bp
  • T to A, chromosome 8 at 106,718,090 bp
  • T to C, chromosome 8 at 112,001,720 bp
  • T to C, chromosome 8 at 124,370,585 bp
  • T to C, chromosome 9 at 65,055,918 bp
  • A to C, chromosome 9 at 75,635,446 bp
  • T to G, chromosome 9 at 119,068,440 bp
  • C to T, chromosome 10 at 69,898,117 bp
  • C to T, chromosome 10 at 76,356,185 bp
  • C to G, chromosome 10 at 80,804,113 bp
  • T to C, chromosome 10 at 89,413,772 bp
  • T to C, chromosome 10 at 107,525,734 bp
  • T to C, chromosome 10 at 127,064,890 bp
  • C to T, chromosome 11 at 32,739,226 bp
  • T to C, chromosome 11 at 67,093,538 bp
  • A to G, chromosome 11 at 73,196,653 bp
  • T to A, chromosome 11 at 97,551,942 bp
  • T to C, chromosome 11 at 102,005,022 bp
  • T to C, chromosome 11 at 110,086,474 bp
  • A to T, chromosome 11 at 114,996,905 bp
  • T to A, chromosome 11 at 121,250,353 bp
  • C to A, chromosome 12 at 54,065,835 bp
  • G to A, chromosome 12 at 54,770,743 bp
  • A to G, chromosome 12 at 55,566,016 bp
  • T to A, chromosome 12 at 57,643,140 bp
  • A to G, chromosome 12 at 103,984,664 bp
  • G to A, chromosome 12 at 112,177,794 bp
  • A to T, chromosome 14 at 24,154,361 bp
  • G to A, chromosome 14 at 52,463,283 bp
  • C to T, chromosome 14 at 60,280,189 bp
  • G to A, chromosome 14 at 100,022,957 bp
  • A to G, chromosome 15 at 28,326,557 bp
  • G to A, chromosome 15 at 28,387,904 bp
  • T to A, chromosome 15 at 76,295,379 bp
  • C to A, chromosome 15 at 77,773,340 bp
  • T to C, chromosome 15 at 101,869,563 bp
  • T to A, chromosome 17 at 5,342,843 bp
  • A to T, chromosome 17 at 15,498,216 bp
  • A to G, chromosome 17 at 24,208,536 bp
  • G to A, chromosome 17 at 57,443,918 bp
  • A to G, chromosome 17 at 94,740,532 bp
  • G to A, chromosome 18 at 12,724,942 bp
  • G to T, chromosome 18 at 14,813,542 bp
  • T to C, chromosome 18 at 20,050,157 bp
  • G to T, chromosome 18 at 63,157,262 bp
  • T to C, chromosome 18 at 82,956,094 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4946 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042543-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.