Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR4947Btlr/Mmmh
Stock Number:
042544-MU
Citation ID:
RRID:MMRRC_042544-MU
Other Names:
R4947 (G1), C57BL/6J-MtgxR4947Btlr
Major Collection:

Strain Information

Myo5a
Name: myosin VA
Synonyms: MVa, MyoVA, Myo5, flail, 9630007J19Rik, Dbv
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 17918
HGNC: HGNC:7602
Homologene: 20100
Acvr1c
Name: activin A receptor, type IC
Synonyms: ALK7, Alk-7
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 269275
Homologene: 26724
Grm1
Name: glutamate receptor, metabotropic 1
Synonyms: mGluR1, Grm1, Gprc1a, nmf373, rcw, 4930455H15Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 14816
HGNC: HGNC:4593
Homologene: 649
D630045J12Rik
Name: RIKEN cDNA D630045J12 gene
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 330286
Homologene: 19782
Unc93b1
Name: unc-93 homolog B1, TLR signaling regulator
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 54445
Homologene: 41325
Strn
Name: striatin, calmodulin binding protein
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 268980
Homologene: 2380
Ctbp2
Name: C-terminal binding protein 2
Synonyms: D7Ertd45e, Ribeye, Gtrgeo6
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 13017
HGNC: HGNC:2495
Homologene: 75187
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 8,782,798 bp
  • T to A, chromosome 1 at 58,676,539 bp
  • A to T, chromosome 1 at 84,038,260 bp
  • G to A, chromosome 1 at 85,112,482 bp
  • CAGAGAGAGAGAGA to CAGAGAGAGAGA, chromosome 1 at 88,211,984 bp
  • G to A, chromosome 1 at 139,865,992 bp
  • A to G, chromosome 1 at 173,755,750 bp
  • C to T, chromosome 2 at 37,795,331 bp
  • T to C, chromosome 2 at 44,591,956 bp
  • T to G, chromosome 2 at 53,073,710 bp
  • T to A, chromosome 2 at 58,315,975 bp
  • T to C, chromosome 2 at 132,373,653 bp
  • A to T, chromosome 2 at 155,024,959 bp
  • T to C, chromosome 2 at 156,254,270 bp
  • T to A, chromosome 2 at 158,186,196 bp
  • A to G, chromosome 3 at 51,856,539 bp
  • T to C, chromosome 3 at 89,226,431 bp
  • T to A, chromosome 3 at 137,925,109 bp
  • A to G, chromosome 3 at 158,063,835 bp
  • A to G, chromosome 4 at 70,228,592 bp
  • A to G, chromosome 4 at 82,966,134 bp
  • A to T, chromosome 4 at 85,764,800 bp
  • T to C, chromosome 4 at 114,191,510 bp
  • G to A, chromosome 5 at 24,377,855 bp
  • A to G, chromosome 5 at 57,721,916 bp
  • T to C, chromosome 5 at 108,487,961 bp
  • T to C, chromosome 5 at 136,057,245 bp
  • C to T, chromosome 6 at 38,148,543 bp
  • A to G, chromosome 6 at 42,141,566 bp
  • C to A, chromosome 6 at 87,903,473 bp
  • A to G, chromosome 6 at 101,796,773 bp
  • T to C, chromosome 6 at 120,402,559 bp
  • A to G, chromosome 7 at 28,089,812 bp
  • G to T, chromosome 7 at 28,310,044 bp
  • A to T, chromosome 7 at 41,400,495 bp
  • A to G, chromosome 7 at 89,633,014 bp
  • A to G, chromosome 7 at 104,938,742 bp
  • T to C, chromosome 7 at 110,825,320 bp
  • A to G, chromosome 7 at 126,367,938 bp
  • A to T, chromosome 7 at 128,712,696 bp
  • T to A, chromosome 7 at 129,616,604 bp
  • A to T, chromosome 7 at 130,625,899 bp
  • T to A, chromosome 7 at 131,357,614 bp
  • C to A, chromosome 7 at 132,999,283 bp
  • C to A, chromosome 8 at 70,844,429 bp
  • T to G, chromosome 9 at 21,237,553 bp
  • A to C, chromosome 9 at 36,868,875 bp
  • A to G, chromosome 9 at 75,123,048 bp
  • T to C, chromosome 9 at 85,710,412 bp
  • G to A, chromosome 9 at 88,957,282 bp
  • A to G, chromosome 10 at 10,782,633 bp
  • T to C, chromosome 10 at 57,523,045 bp
  • T to G, chromosome 10 at 79,194,698 bp
  • A to T, chromosome 10 at 97,682,148 bp
  • C to T, chromosome 11 at 73,375,417 bp
  • T to C, chromosome 11 at 97,138,944 bp
  • T to C, chromosome 11 at 101,575,077 bp
  • C to A, chromosome 11 at 115,609,637 bp
  • T to C, chromosome 11 at 116,223,375 bp
  • A to T, chromosome 12 at 27,160,228 bp
  • A to G, chromosome 12 at 98,759,824 bp
  • T to C, chromosome 13 at 67,596,672 bp
  • T to C, chromosome 13 at 100,539,715 bp
  • A to C, chromosome 14 at 24,482,464 bp
  • T to A, chromosome 14 at 51,315,959 bp
  • T to A, chromosome 15 at 28,272,372 bp
  • T to C, chromosome 15 at 74,851,570 bp
  • T to C, chromosome 16 at 36,321,765 bp
  • A to G, chromosome 16 at 59,027,445 bp
  • A to T, chromosome 16 at 91,682,551 bp
  • T to A, chromosome 17 at 22,602,879 bp
  • A to G, chromosome 17 at 32,368,083 bp
  • T to A, chromosome 17 at 35,359,732 bp
  • T to C, chromosome 17 at 37,466,743 bp
  • T to A, chromosome 17 at 56,010,268 bp
  • T to C, chromosome 17 at 78,661,779 bp
  • A to T, chromosome 17 at 84,771,538 bp
  • T to C, chromosome 18 at 65,625,066 bp
  • C to A, chromosome 19 at 3,935,871 bp
  • T to C, chromosome 19 at 8,131,452 bp
  • T to C, chromosome 19 at 8,946,291 bp
  • T to C, chromosome 19 at 10,866,934 bp
  • T to C, chromosome 19 at 11,454,737 bp
  • T to C, chromosome 19 at 29,700,151 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4947 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042544-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.