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Strain Name:
C57BL/6J-MtgxR4954Btlr/Mmmh
Stock Number:
042551-MU
Citation ID:
RRID:MMRRC_042551-MU
Other Names:
R4954 (G1), C57BL/6J-MtgxR4954Btlr
Major Collection:

Strain Information

Ext1
Name: exostosin glycosyltransferase 1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 14042
HGNC: HGNC:3512
Homologene: 30957
Sema7a
Name: sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7A
Synonyms: Semaphorin K1, CDw108, Semal, 2900057C09Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 20361
VEGA: 9
Homologene: 2678
Lgi1
Name: leucine-rich repeat LGI family, member 1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 56839
HGNC: HGNC:6572
Homologene: 3737
Ncoa3
Name: nuclear receptor coactivator 3
Synonyms: pCIP, TRAM-1, AIB1, RAC3, TRAM1, Src3, 2010305B15Rik, KAT13B, bHLHe42
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 17979
HGNC: HGNC:7670
Homologene: 4764
Phf14
Name: PHD finger protein 14
Synonyms: 1110001C23Rik, 4932409F11Rik, 5730446A07Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 75725
Homologene: 8775
Rps6ka2
Name: ribosomal protein S6 kinase, polypeptide 2
Synonyms: Rsk3, Rps6ka-rs1, 90kDa, p90rsk, pp90rsk, D17Wsu134e
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 20112
VEGA: 17
Homologene: 100680
Usp5
Name: ubiquitin specific peptidase 5 (isopeptidase T)
Synonyms: Ucht
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 22225
Homologene: 55758
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 36,692,456 bp
  • T to C, chromosome 1 at 92,644,445 bp
  • A to G, chromosome 1 at 139,053,386 bp
  • T to C, chromosome 2 at 52,177,518 bp
  • T to G, chromosome 2 at 66,095,876 bp
  • T to A, chromosome 2 at 73,310,504 bp
  • C to T, chromosome 2 at 91,055,629 bp
  • C to A, chromosome 2 at 164,151,681 bp
  • T to A, chromosome 2 at 164,151,682 bp
  • A to T, chromosome 2 at 165,514,755 bp
  • T to C, chromosome 2 at 166,065,786 bp
  • T to A, chromosome 3 at 40,785,400 bp
  • T to G, chromosome 3 at 56,035,958 bp
  • T to A, chromosome 3 at 64,140,484 bp
  • A to T, chromosome 3 at 129,284,180 bp
  • G to A, chromosome 4 at 32,821,284 bp
  • G to A, chromosome 4 at 41,498,241 bp
  • A to T, chromosome 4 at 43,985,077 bp
  • C to T, chromosome 4 at 58,344,222 bp
  • G to A, chromosome 4 at 109,370,678 bp
  • T to C, chromosome 4 at 121,395,001 bp
  • A to T, chromosome 4 at 123,322,459 bp
  • T to A, chromosome 4 at 134,934,972 bp
  • A to T, chromosome 4 at 144,190,732 bp
  • A to T, chromosome 5 at 28,339,276 bp
  • G to T, chromosome 5 at 29,757,681 bp
  • A to T, chromosome 5 at 52,819,217 bp
  • C to T, chromosome 5 at 97,086,764 bp
  • A to T, chromosome 5 at 145,370,955 bp
  • C to A, chromosome 6 at 11,987,620 bp
  • A to G, chromosome 6 at 43,236,384 bp
  • C to G, chromosome 6 at 124,822,630 bp
  • A to T, chromosome 6 at 131,685,005 bp
  • A to T, chromosome 7 at 23,361,893 bp
  • CTT to CT, chromosome 7 at 44,591,296 bp
  • T to C, chromosome 7 at 86,306,601 bp
  • T to C, chromosome 7 at 99,238,517 bp
  • A to G, chromosome 7 at 103,732,207 bp
  • C to T, chromosome 7 at 130,509,018 bp
  • A to T, chromosome 9 at 36,432,860 bp
  • T to C, chromosome 9 at 57,956,380 bp
  • C to T, chromosome 9 at 119,758,659 bp
  • G to T, chromosome 10 at 123,131,398 bp
  • G to A, chromosome 11 at 69,539,496 bp
  • G to A, chromosome 11 at 101,332,099 bp
  • A to G, chromosome 12 at 45,437,600 bp
  • G to A, chromosome 12 at 91,299,036 bp
  • C to A, chromosome 12 at 110,658,126 bp
  • T to C, chromosome 12 at 116,256,025 bp
  • G to A, chromosome 12 at 118,911,434 bp
  • G to A, chromosome 13 at 12,407,516 bp
  • T to C, chromosome 15 at 53,344,492 bp
  • T to A, chromosome 15 at 85,816,401 bp
  • T to C, chromosome 16 at 36,033,326 bp
  • A to G, chromosome 17 at 7,271,604 bp
  • G to A, chromosome 17 at 7,299,286 bp
  • C to A, chromosome 17 at 36,041,959 bp
  • C to A, chromosome 17 at 67,869,013 bp
  • C to T, chromosome 17 at 71,902,692 bp
  • T to A, chromosome 17 at 74,612,031 bp
  • T to A, chromosome 18 at 39,243,641 bp
  • C to T, chromosome 19 at 38,284,157 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4954 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042551-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.