Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR4985Btlr/Mmmh
Stock Number:
042579-MU
Citation ID:
RRID:MMRRC_042579-MU
Other Names:
R4985 (G1), C57BL/6J-MtgxR4985Btlr
Major Collection:

Strain Information

Htr3b
Name: 5-hydroxytryptamine (serotonin) receptor 3B
Synonyms: 5-HT3 receptor subunit B, 5-HT3B
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 57014
VEGA: 9
HGNC: HGNC:5298
Homologene: 38131
Ldb2
Name: LIM domain binding 2
Synonyms: CLIM1, Ldb3, CLP-36, CLIM-1b, CLIM-1a
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 16826
HGNC: HGNC:6533
Homologene: 989
Dlg1
Name: discs large MAGUK scaffold protein 1
Synonyms: B130052P05Rik, SAP97, Dlgh1
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 13383
HGNC: HGNC:2900
Homologene: 20869
Nap1l1
Name: nucleosome assembly protein 1-like 1
Synonyms: D10Ertd68e
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 53605
VEGA: 10
HGNC: HGNC:7637
Homologene: 129218
Pcf11
Name: PCF11 cleavage and polyadenylation factor subunit
Synonyms: 5730417B17Rik, 2500001H09Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 74737
Homologene: 32282
Pafah1b1
Name: platelet-activating factor acetylhydrolase, isoform 1b, subunit 1
Synonyms: Lis1, LIS-1, lissencephaly-1 protein, Mdsh, Pafaha, PAF-AH 45
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18472
HGNC: HGNC:8574
Homologene: 371
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 153,136,805 bp
  • T to C, chromosome 2 at 91,212,298 bp
  • A to G, chromosome 2 at 155,814,096 bp
  • G to A, chromosome 2 at 180,603,400 bp
  • C to T, chromosome 3 at 95,736,103 bp
  • A to T, chromosome 4 at 22,487,588 bp
  • T to C, chromosome 4 at 45,040,692 bp
  • A to G, chromosome 4 at 59,691,017 bp
  • A to G, chromosome 4 at 133,612,917 bp
  • T to A, chromosome 5 at 44,480,303 bp
  • T to C, chromosome 5 at 114,622,732 bp
  • A to T, chromosome 7 at 22,843,534 bp
  • A to G, chromosome 7 at 28,918,986 bp
  • T to C, chromosome 7 at 92,661,902 bp
  • C to A, chromosome 7 at 107,129,027 bp
  • T to C, chromosome 7 at 113,285,073 bp
  • A to C, chromosome 7 at 141,213,277 bp
  • A to C, chromosome 7 at 141,438,591 bp
  • C to T, chromosome 8 at 22,243,415 bp
  • G to T, chromosome 8 at 47,713,445 bp
  • T to C, chromosome 8 at 93,175,144 bp
  • C to T, chromosome 8 at 105,728,011 bp
  • T to G, chromosome 8 at 123,267,907 bp
  • T to A, chromosome 9 at 38,594,362 bp
  • C to A, chromosome 9 at 48,935,941 bp
  • T to C, chromosome 9 at 108,565,210 bp
  • T to C, chromosome 10 at 10,400,632 bp
  • G to A, chromosome 10 at 34,282,338 bp
  • T to C, chromosome 10 at 111,490,083 bp
  • T to C, chromosome 10 at 127,199,375 bp
  • C to A, chromosome 11 at 16,859,029 bp
  • C to A, chromosome 11 at 61,302,233 bp
  • T to G, chromosome 11 at 74,685,988 bp
  • T to A, chromosome 11 at 77,138,229 bp
  • T to A, chromosome 11 at 83,187,207 bp
  • C to A, chromosome 11 at 99,858,448 bp
  • A to T, chromosome 12 at 34,874,458 bp
  • A to T, chromosome 12 at 102,368,562 bp
  • A to G, chromosome 12 at 103,653,936 bp
  • C to A, chromosome 12 at 110,658,126 bp
  • G to T, chromosome 12 at 112,969,370 bp
  • T to A, chromosome 13 at 22,557,060 bp
  • T to G, chromosome 14 at 31,286,898 bp
  • A to T, chromosome 15 at 101,477,265 bp
  • A to G, chromosome 16 at 31,788,135 bp
  • T to A, chromosome 17 at 25,876,776 bp
  • T to C, chromosome 17 at 29,626,860 bp
  • T to C, chromosome 17 at 70,844,872 bp
  • T to C, chromosome 18 at 37,715,645 bp
  • C to T, chromosome 19 at 5,069,792 bp
  • C to A, chromosome 19 at 45,150,982 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4985 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042579-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.