Strain Name:
C57BL/6J-MtgxR4985Btlr/Mmmh
Stock Number:
042579-MU
Citation ID:
RRID:MMRRC_042579-MU
Other Names:
R4985 (G1), C57BL/6J-MtgxR4985Btlr
Major Collection:

Strain Information

Htr3b
Name: 5-hydroxytryptamine (serotonin) receptor 3B
Synonyms: 5-HT3 receptor subunit B, 5-HT3B
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 57014
VEGA: 9
HGNC: HGNC:5298
Homologene: 38131
Ldb2
Name: LIM domain binding 2
Synonyms: CLIM1, Ldb3, CLP-36, CLIM-1b, CLIM-1a
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 16826
HGNC: HGNC:6533
Homologene: 989
Dlg1
Name: discs large MAGUK scaffold protein 1
Synonyms: B130052P05Rik, SAP97, Dlgh1
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 13383
HGNC: HGNC:2900
Homologene: 20869
Nap1l1
Name: nucleosome assembly protein 1-like 1
Synonyms: D10Ertd68e
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 53605
VEGA: 10
HGNC: HGNC:7637
Homologene: 129218
Pcf11
Name: PCF11 cleavage and polyadenylation factor subunit
Synonyms: 5730417B17Rik, 2500001H09Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 74737
Homologene: 32282
Pafah1b1
Name: platelet-activating factor acetylhydrolase, isoform 1b, subunit 1
Synonyms: Lis1, LIS-1, lissencephaly-1 protein, Mdsh, Pafaha, PAF-AH 45
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18472
HGNC: HGNC:8574
Homologene: 371
Dync1h1
Name: dynein cytoplasmic 1 heavy chain 1
Synonyms: MAP1C, dynein heavy chain, retrograde transport, Dnec1, Loa, 9930018I23Rik, Dnchc1, Swl
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 13424
HGNC: HGNC:2961
Homologene: 1053
Bmal1
Name: basic helix-loop-helix ARNT like 1
Synonyms: Arnt3, MOP3, bHLHe5, Arntl
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 11865
HGNC: HGNC:701
Homologene: 910
Pip4k2c
Name: phosphatidylinositol-5-phosphate 4-kinase, type II, gamma
Synonyms: Pip5k2c
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 117150
VEGA: 10
Homologene: 23484
Tgif1
Name: TGFB-induced factor homeobox 1
Synonyms: Tgif
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 21815
Homologene: 7574
Impdh2
Name: inosine monophosphate dehydrogenase 2
Synonyms: IMP dehydrogenase type II
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 23918
HGNC: HGNC:6053
Homologene: 48919
Zdhhc18
Name: zinc finger, DHHC domain containing 18
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 503610
Homologene: 110918
Egfr
Name: epidermal growth factor receptor
Synonyms: avian erythroblastic leukemia viral (v-erb-b) oncogene homolog, Erbb, Wa5, 9030024J15Rik, Errb1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 13649
HGNC: HGNC:3236
Homologene: 74545
Trpv4
Name: transient receptor potential cation channel, subfamily V, member 4
Synonyms: Trp12, VR-OAC, 0610033B08Rik, OTRPC4, VROAC, VRL-2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 63873
Homologene: 11003
Thsd1
Name: thrombospondin, type I, domain 1
Synonyms: 4833423O18Rik, Tmtsp
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 56229
Homologene: 10264
Dnah1
Name: dynein, axonemal, heavy chain 1
Synonyms: MDHC7, E030034C22Rik, B230373P09Rik, Dnahc1, G1-415-19, ferf1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 110084
VEGA: 14
HGNC: HGNC:2940
Homologene: 67131
Col9a3
Name: collagen, type IX, alpha 3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 12841
HGNC: HGNC:2219
Homologene: 20438
Vmn1r204
Name: vomeronasal 1 receptor 204
Synonyms: Gm11301
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 632793
Homologene: 110880
Krt86
Name: keratin 86
Synonyms: MHb4, Khb4, Krt2-11, Krt2-10
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 16679
VEGA: 15
HGNC: HGNC:6463
Homologene: 1717
Slc47a2
Name: solute carrier family 47, member 2
Synonyms: 4933429E10Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 380701
Homologene: 134426
Ddb2
Name: damage specific DNA binding protein 2
Synonyms: p48, 2610043A19Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 107986
HGNC: HGNC:2718
Homologene: 83
Adgb
Name: androglobin
Synonyms: 9130014G24Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 215772
Homologene: 100289
Zfp276
Name: zinc finger protein (C2H2 type) 276
Synonyms: D8Ertd370e, D8Ertd377e
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 57247
Homologene: 10731
Brf1
Name: BRF1, RNA polymerase III transcription initiation factor 90 kDa subunit
Synonyms: TAF3C, TFIIIB90, GTF3B, TAFIII90, 2510002F24Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 72308
VEGA: 12
Homologene: 1161
Lmntd2
Name: lamin tail domain containing 2
Synonyms: 1600016N20Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 72000
Homologene: 104159
Ces1d
Name: carboxylesterase 1D
Synonyms: TGH, Ces3
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 104158
HGNC: HGNC:1863
Homologene: 35606
Cd248
Name: CD248 antigen, endosialin
Synonyms: 2610111G01Rik, Tem1, Cd164l1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 70445
VEGA: 19
Homologene: 10699
Tspyl1
Name: testis-specific protein, Y-encoded-like 1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 22110
Homologene: 22522
Pou3f2
Name: POU domain, class 3, transcription factor 2
Synonyms: Brn-2, Brn2, Otf7, 9430075J19Rik, A230098E07Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 18992
HGNC: HGNC:9215
Homologene: 4095
Serpina6
Name: serine (or cysteine) peptidase inhibitor, clade A, member 6
Synonyms: Cbg
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 12401
HGNC: HGNC:1540
Homologene: 20417
Efcab5
Name: EF-hand calcium binding domain 5
Synonyms: 4930563A03Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 319634
Homologene: 63366
Lamc2
Name: laminin, gamma 2
Synonyms: nicein, 100kDa
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 16782
HGNC: HGNC:6493
Homologene: 4062
Pidd1
Name: p53 induced death domain protein 1
Synonyms: 1200011D09Rik, Pidd, Lrdd
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 57913
Homologene: 11220
Ecm1
Name: extracellular matrix protein 1
Synonyms: p85
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 13601
HGNC: HGNC:3153
Homologene: 3260
Or2d2
Name: olfactory receptor family 2 subfamily D member 2
Synonyms: GA_x6K02T2PBJ9-9479517-9478573, MOR260-1, Olfr715
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258776
HGNC: HGNC:8244
Homologene: 81541
Rnf8
Name: ring finger protein 8
Synonyms: AIP37, 3830404E21Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 58230
Homologene: 2944
Mmp24
Name: matrix metallopeptidase 24
Synonyms: MT5-MMP, Membrane type 5-MMP
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 17391
HGNC: HGNC:7172
Homologene: 21331
Fbxo10
Name: F-box protein 10
Synonyms: FBX10, LOC269529
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 269529
Homologene: 19544
Vmn1r159
Name: vomeronasal 1 receptor 159
Synonyms: Gm16507
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 670857
Homologene: 104166
Tlx1
Name: T cell leukemia, homeobox 1
Synonyms: Hox11, Hox-11
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 21908
HGNC: HGNC:5056
Homologene: 4031
Gfod2
Name: glucose-fructose oxidoreductase domain containing 2
Synonyms: 5730466C23Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 70575
Homologene: 10361
Or8b49
Name: olfactory receptor family 8 subfamily B member 49
Synonyms: GA_x6K02T2PVTD-32296575-32297513, MOR165-9P, MOR165-10, Olfr913
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 258225
VEGA: 9
Homologene: 79398
Pcdhga7
Name: protocadherin gamma subfamily A, 7
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 93715
HGNC: HGNC:8705
Homologene: 36377
E030037K01Rik
Name: RIKEN cDNA E030037K01 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
4921508M14Rik
Name: RIKEN cDNA 4921508M14 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Mettl26
Name: methyltransferase like 26
Synonyms: 0610011F06Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 68347
Homologene: 16917
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 153,136,805 bp
  • T to C, chromosome 2 at 91,212,298 bp
  • A to G, chromosome 2 at 155,814,096 bp
  • G to A, chromosome 2 at 180,603,400 bp
  • C to T, chromosome 3 at 95,736,103 bp
  • A to T, chromosome 4 at 22,487,588 bp
  • T to C, chromosome 4 at 45,040,692 bp
  • A to G, chromosome 4 at 59,691,017 bp
  • A to G, chromosome 4 at 133,612,917 bp
  • T to A, chromosome 5 at 44,480,303 bp
  • T to C, chromosome 5 at 114,622,732 bp
  • A to T, chromosome 7 at 22,843,534 bp
  • A to G, chromosome 7 at 28,918,986 bp
  • T to C, chromosome 7 at 92,661,902 bp
  • C to A, chromosome 7 at 107,129,027 bp
  • T to C, chromosome 7 at 113,285,073 bp
  • A to C, chromosome 7 at 141,213,277 bp
  • A to C, chromosome 7 at 141,438,591 bp
  • C to T, chromosome 8 at 22,243,415 bp
  • G to T, chromosome 8 at 47,713,445 bp
  • T to C, chromosome 8 at 93,175,144 bp
  • C to T, chromosome 8 at 105,728,011 bp
  • T to G, chromosome 8 at 123,267,907 bp
  • T to A, chromosome 9 at 38,594,362 bp
  • C to A, chromosome 9 at 48,935,941 bp
  • T to C, chromosome 9 at 108,565,210 bp
  • T to C, chromosome 10 at 10,400,632 bp
  • G to A, chromosome 10 at 34,282,338 bp
  • T to C, chromosome 10 at 111,490,083 bp
  • T to C, chromosome 10 at 127,199,375 bp
  • C to A, chromosome 11 at 16,859,029 bp
  • C to A, chromosome 11 at 61,302,233 bp
  • T to G, chromosome 11 at 74,685,988 bp
  • T to A, chromosome 11 at 77,138,229 bp
  • T to A, chromosome 11 at 83,187,207 bp
  • C to A, chromosome 11 at 99,858,448 bp
  • A to T, chromosome 12 at 34,874,458 bp
  • A to T, chromosome 12 at 102,368,562 bp
  • A to G, chromosome 12 at 103,653,936 bp
  • C to A, chromosome 12 at 110,658,126 bp
  • G to T, chromosome 12 at 112,969,370 bp
  • T to A, chromosome 13 at 22,557,060 bp
  • T to G, chromosome 14 at 31,286,898 bp
  • A to T, chromosome 15 at 101,477,265 bp
  • A to G, chromosome 16 at 31,788,135 bp
  • T to A, chromosome 17 at 25,876,776 bp
  • T to C, chromosome 17 at 29,626,860 bp
  • T to C, chromosome 17 at 70,844,872 bp
  • T to C, chromosome 18 at 37,715,645 bp
  • C to T, chromosome 19 at 5,069,792 bp
  • C to A, chromosome 19 at 45,150,982 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4985 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042579-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.