Strain Name:
C57BL/6J-MtgxR4986Btlr/Mmmh
Stock Number:
042580-MU
Citation ID:
RRID:MMRRC_042580-MU
Other Names:
R4986 (G1), C57BL/6J-MtgxR4986Btlr
Major Collection:

Gene Information

Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 11426
Homologene: 136191
Igf2bp2
Name: insulin-like growth factor 2 mRNA binding protein 2
Synonyms: IMP-2, C330012H03Rik, IMP2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 319765
Homologene: 4774
Wdfy3
Name: WD repeat and FYVE domain containing 3
Synonyms: Ggtb3, 2610509D04Rik, D5Ertd66e, Bwf1, Bchs, Alfy
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 72145
Homologene: 22855
Septin11
Name: septin 11
Synonyms: 6230410I01Rik, D5Ertd606e, Sept11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 52398
Homologene: 56800
Emc2
Name: ER membrane protein complex subunit 2
Synonyms: 4921531G14Rik, Ttc35
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 66736
VEGA: 15
Homologene: 8785
Ccdc34
Name: coiled-coil domain containing 34
Synonyms: 4930522P10Rik, 2810027O19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 68201
Homologene: 12245
Ubtf
Name: upstream binding transcription factor, RNA polymerase I
Synonyms: UBF1, Tcfubf, UBF, A930005G04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 21429
Homologene: 7970
Mdh1
Name: malate dehydrogenase 1, NAD (soluble)
Synonyms: Mor-2, MDH-s, Mor2, B230377B03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 17449
HGNC: HGNC:6970
Homologene: 4324
Ybx1
Name: Y box protein 1
Synonyms: MSY1, DNA binding protein B, Nsep1, dbpB, EF1A, YB-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 22608
HGNC: HGNC:8014
Homologene: 88707
Gad1
Name: glutamate decarboxylase 1
Synonyms: GAD67, Gad-1, Z49976, EP10, GAD44, GAD25
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 14415
HGNC: HGNC:4092
Homologene: 635
Slain1
Name: SLAIN motif family, member 1
Synonyms: 9630044O09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 105439
Homologene: 16943
Armh4
Name: armadillo-like helical domain containing 4
Synonyms: 3632451O06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 67419
Homologene: 12127
Mecom
Name: MDS1 and EVI1 complex locus
Synonyms: ZNFPR1B1, Prdm3, MDS1-EVI1, Evi-1, D630039M04Rik, Jbo, Evi1, Mds1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 14013
HGNC: HGNC:3498
Homologene: 21086
Zfp993
Name: zinc finger protein 993
Synonyms: Gm13248
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 100503000
Itpr2
Name: inositol 1,4,5-triphosphate receptor 2
Synonyms: Ip3r2, Itpr5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 16439
HGNC: HGNC:6181
Homologene: 37593
Muc4
Name: mucin 4
Synonyms: 4933405I11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 140474
HGNC: HGNC:7514
Homologene: 124469
Fat3
Name: FAT atypical cadherin 3
Synonyms: LOC234973, LOC382129, D430038H04Rik, 9430076A06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 270120
VEGA: 9
Homologene: 82252
Dock3
Name: dedicator of cyto-kinesis 3
Synonyms: PBP, Moca
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 208869
HGNC: HGNC:2989
Homologene: 21030
Tmem132e
Name: transmembrane protein 132E
Synonyms: LOC270893
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 270893
Homologene: 41524
Abcc9
Name: ATP-binding cassette, sub-family C (CFTR/MRP), member 9
Synonyms: SUR2B, SUR2A, Sur2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 20928
HGNC: HGNC:60
Homologene: 56521
Igsf10
Name: immunoglobulin superfamily, member 10
Synonyms: 6530405F15Rik, CMF608, Adlican2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 242050
Homologene: 18712
Sacs
Name: sacsin
Synonyms: E130115J16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 50720
Zfp944
Name: zinc finger protein 944
Synonyms: 6330416L07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 319615
VEGA: 17
Homologene: 133237
Olfr1151
Name: olfactory receptor 1151
Synonyms: GA_x6K02T2Q125-49358694-49359620, MOR177-9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258631
Homologene: 36999
Osmr
Name: oncostatin M receptor
Synonyms: OSMRB
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 18414
HGNC: HGNC:8507
Homologene: 2972
Rrs1
Name: ribosome biogenesis regulator 1
Synonyms: 5730466A07Rik, D1Ertd701e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 59014
Homologene: 41014
Skint9
Name: selection and upkeep of intraepithelial T cells 9
Synonyms: A030013N09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 329918
Homologene: 136292
Ceacam5
Name: carcinoembryonic antigen-related cell adhesion molecule 5
Synonyms: 1600029H12Rik, Psg30
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 73250
Homologene: 115938
Sp110
Name: Sp110 nuclear body protein
Synonyms: 52kDa, Ipr1, 5031415C07Rik, 5830484A20Rik, Ifi75
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 109032
HGNC: HGNC:5401
Homologene: 82192
Ces2f
Name: carboxylesterase 2F
Synonyms: 2310038E17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 71903
HGNC: HGNC:1864
Homologene: 135673
Srl
Name: sarcalumenin
Synonyms: sarcalumenin, sar, 9830004M20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 106393
Homologene: 45500
Slc36a3
Name: solute carrier family 36 (proton/amino acid symporter), member 3
Synonyms: tramdorin2, PAT3, TRAMD2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 215332
Homologene: 76175
Gpr137c
Name: G protein-coupled receptor 137C
Synonyms: LOC380893, TM7SF1L2, 6330416L11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 70713
Homologene: 28613
Defa30
Name: defensin, alpha, 30
Synonyms: Gm15284
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 100038927
Homologene: 113382
Olfr1265
Name: olfactory receptor 1265
Synonyms: GA_x6K02T2Q125-51469027-51469956, MOR228-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258340
Homologene: 133727
Gm9944
Name: predicted gene 9944
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Gm20528
Name: predicted gene 20528
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
AC124745.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 9,545,767 bp
  • G to A, chromosome 1 at 85,591,760 bp
  • CAGAGAGAGAGAGA to CAGAGAGAGAGA, chromosome 1 at 88,211,984 bp
  • A to G, chromosome 2 at 70,600,693 bp
  • T to A, chromosome 2 at 87,857,514 bp
  • A to T, chromosome 2 at 90,037,428 bp
  • T to C, chromosome 2 at 110,017,869 bp
  • G to T, chromosome 3 at 29,980,699 bp
  • T to C, chromosome 3 at 59,328,606 bp
  • T to A, chromosome 4 at 112,391,713 bp
  • C to T, chromosome 4 at 119,282,430 bp
  • C to T, chromosome 4 at 123,391,121 bp
  • T to C, chromosome 4 at 144,453,190 bp
  • T to A, chromosome 4 at 146,657,557 bp
  • T to C, chromosome 5 at 93,161,241 bp
  • C to T, chromosome 5 at 101,943,119 bp
  • A to T, chromosome 6 at 142,627,591 bp
  • T to A, chromosome 6 at 146,240,342 bp
  • T to A, chromosome 7 at 17,757,833 bp
  • T to A, chromosome 8 at 21,135,416 bp
  • G to A, chromosome 8 at 104,952,025 bp
  • T to C, chromosome 9 at 15,998,340 bp
  • A to C, chromosome 9 at 106,931,983 bp
  • A to G, chromosome 11 at 21,558,545 bp
  • T to A, chromosome 11 at 55,146,766 bp
  • T to C, chromosome 11 at 82,434,256 bp
  • G to A, chromosome 11 at 102,314,174 bp
  • A to T, chromosome 14 at 45,246,286 bp
  • A to T, chromosome 14 at 49,751,654 bp
  • T to A, chromosome 14 at 61,213,043 bp
  • A to G, chromosome 14 at 79,452,609 bp
  • A to T, chromosome 14 at 103,688,105 bp
  • A to G, chromosome 15 at 6,816,580 bp
  • A to G, chromosome 15 at 43,511,784 bp
  • T to C, chromosome 16 at 4,496,782 bp
  • C to T, chromosome 16 at 22,070,306 bp
  • A to G, chromosome 16 at 32,777,635 bp
  • A to T, chromosome 17 at 22,339,230 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4986 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042580-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.