Strain Name:
C57BL/6J-MtgxR4987Btlr/Mmmh
Stock Number:
042581-MU
Citation ID:
RRID:MMRRC_042581-MU
Other Names:
R4987 (G1), C57BL/6J-MtgxR4987Btlr
Major Collection:

Gene Information

Nos1
Name: nitric oxide synthase 1, neuronal
Synonyms: nNOS, bNOS, Nos-1, NO, 2310005C01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 18125
HGNC: HGNC:7872
Homologene: 37327
Ahdc1
Name: AT hook, DNA binding motif, containing 1
Synonyms: D030015G18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230793
Homologene: 17144
Mfap4
Name: microfibrillar-associated protein 4
Synonyms: 1110007F23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 76293
HGNC: HGNC:7035
Homologene: 134529
Camk1g
Name: calcium/calmodulin-dependent protein kinase I gamma
Synonyms: CLICK-III, CaMKIgamma
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 215303
Homologene: 48706
Rbm25
Name: RNA binding motif protein 25
Synonyms: 2610015J01Rik, A130095G20Rik, 2600011C06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 67039
Lin9
Name: lin-9 DREAM MuvB core complex component
Synonyms: 2700022J23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 72568
Homologene: 35252
Ubr1
Name: ubiquitin protein ligase E3 component n-recognin 1
Synonyms: E3 alpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22222
Homologene: 7582
Lpcat1
Name: lysophosphatidylcholine acyltransferase 1
Synonyms: 2900035H07Rik, LPCAT, Aytl2, rd11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 210992
Homologene: 9871
Adcy4
Name: adenylate cyclase 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 104110
VEGA: 14
HGNC: HGNC:235
Homologene: 23149
Zp3
Name: zona pellucida glycoprotein 3
Synonyms: Zp-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 22788
Homologene: 5178
Brms1l
Name: breast cancer metastasis-suppressor 1-like
Synonyms: 0710008O11Rik, BRMS1, D12Ertd407e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 52592
VEGA: 12
Homologene: 9123
Trpv4
Name: transient receptor potential cation channel, subfamily V, member 4
Synonyms: VR-OAC, Trp12, 0610033B08Rik, OTRPC4, VROAC, VRL-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 63873
Homologene: 11003
Ppme1
Name: protein phosphatase methylesterase 1
Synonyms: 1110069N17Rik, 2700017M01Rik, PME-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 72590
Homologene: 6099
Rlbp1
Name: retinaldehyde binding protein 1
Synonyms: CRALBP, 3110056M11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 19771
Homologene: 68046
B3gnt5
Name: UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 108105
Homologene: 24989
Dennd1c
Name: DENN/MADD domain containing 1C
Synonyms: 4432409M07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 70785
VEGA: 17
Homologene: 11773
Smco2
Name: single-pass membrane protein with coiled-coil domains 2
Synonyms: 1700023A16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 69371
Homologene: 106211
Hibadh
Name: 3-hydroxyisobutyrate dehydrogenase
Synonyms: 6430402H10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 58875
HGNC: HGNC:4907
Homologene: 15088
Nutm2
Name: NUT family member 2
Synonyms: LOC328250, Gm806
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 328250
Homologene: 128615
Plcd4
Name: phospholipase C, delta 4
Synonyms: 4921507K24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 18802
HGNC: HGNC:9062
Homologene: 88782
Cspg4b
Name: chondroitin sulfate proteoglycan 4B
Synonyms: BC067074
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 408066
Rusc2
Name: RUN and SH3 domain containing 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 100213
Homologene: 18967
Dpysl3
Name: dihydropyrimidinase-like 3
Synonyms: Ulip1, Ulip, CRMP-4, TUC4, 9430041P20Rik, CRMP4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 22240
HGNC: HGNC:3015
Homologene: 20361
Dscam
Name: DS cell adhesion molecule
Synonyms: 4932410A21Rik, Down syndrome cell adhesion molecule
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 13508
HGNC: HGNC:3039
Homologene: 74393
Or8k33
Name: olfactory receptor family 8 subfamily K member 33
Synonyms: GA_x6K02T2Q125-48039418-48038477, MOR192-1, Olfr1080
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258404
Homologene: 104285
Atp10b
Name: ATPase, class V, type 10B
Synonyms: 5930426O13Rik, 9030605H24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 319767
Homologene: 70969
Chl1
Name: cell adhesion molecule L1-like
Synonyms: CALL, A530023M13Rik, close homolog of L1, LICAM2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 12661
HGNC: HGNC:1939
Homologene: 21314
Acrbp
Name: proacrosin binding protein
Synonyms: sp32, OY-TES-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 54137
Homologene: 9641
Slc25a32
Name: solute carrier family 25, member 32
Synonyms: 2610043O12Rik, Mftc
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 69906
VEGA: 15
Homologene: 6151
Wdsub1
Name: WD repeat, SAM and U-box domain containing 1
Synonyms: 1700048E19Rik, 2610014F08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 72137
Homologene: 17609
Fmo5
Name: flavin containing monooxygenase 5
Synonyms: 5033418D19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 14263
HGNC: HGNC:3773
Homologene: 68185
Gzmc
Name: granzyme C
Synonyms: CCP2, Ctla5, Ctla-5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 14940
VEGA: 14
HGNC: HGNC:4709
Homologene: 105844
Krtap31-2
Name: keratin associated protein 31-2
Synonyms: OTTMUSG00000002177
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 432602
Homologene: 134336
Nostrin
Name: nitric oxide synthase trafficker
Synonyms: mDaIP2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 329416
Homologene: 43142
Krt83
Name: keratin 83
Synonyms: 5430421N21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 100126226
Homologene: 68248
Serinc2
Name: serine incorporator 2
Synonyms: FKSG84, TDE2, 2310004K20Rik, Tde2l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230779
Homologene: 27797
1700057G04Rik
Name: RIKEN cDNA 1700057G04 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 78459
Homologene: 134508
Hax1
Name: HCLS1 associated X-1
Synonyms: mHAX-1s, HAX-1, Silg111, Hs1bp1, HS1-associated protein X-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 23897
Homologene: 4463
Gm9991
Name: predicted gene 9991
Synonyms: EG433332
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 433332
Gm26996
Name: predicted gene, 26996
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Gm10425
Name: predicted gene 10425
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Pcdha12
Name: protocadherin alpha 12
Synonyms: Cnr5, Crnr5, Pcdha13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 192164
HGNC: HGNC:8667
Homologene: 135870
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 74,547,959 bp
  • A to T, chromosome 1 at 90,675,416 bp
  • A to G, chromosome 1 at 180,668,764 bp
  • T to A, chromosome 1 at 193,348,475 bp
  • A to G, chromosome 2 at 59,870,393 bp
  • A to G, chromosome 2 at 69,156,431 bp
  • A to C, chromosome 2 at 86,553,235 bp
  • A to T, chromosome 2 at 120,963,566 bp
  • T to C, chromosome 3 at 89,997,709 bp
  • T to C, chromosome 3 at 97,635,578 bp
  • T to C, chromosome 4 at 43,425,280 bp
  • C to T, chromosome 4 at 130,263,027 bp
  • T to G, chromosome 4 at 133,064,320 bp
  • T to C, chromosome 5 at 114,622,732 bp
  • T to C, chromosome 5 at 117,926,533 bp
  • T to A, chromosome 5 at 135,987,505 bp
  • A to T, chromosome 6 at 52,622,895 bp
  • A to G, chromosome 6 at 103,674,977 bp
  • T to C, chromosome 6 at 125,053,762 bp
  • A to G, chromosome 6 at 130,590,996 bp
  • A to G, chromosome 6 at 146,856,092 bp
  • A to T, chromosome 7 at 79,380,131 bp
  • T to C, chromosome 7 at 100,345,071 bp
  • T to C, chromosome 9 at 92,354,584 bp
  • A to G, chromosome 11 at 43,151,613 bp
  • A to G, chromosome 11 at 61,486,082 bp
  • A to G, chromosome 11 at 99,936,570 bp
  • T to A, chromosome 12 at 55,866,015 bp
  • T to C, chromosome 12 at 83,677,856 bp
  • C to A, chromosome 12 at 111,429,493 bp
  • C to T, chromosome 13 at 50,472,343 bp
  • T to A, chromosome 13 at 73,489,103 bp
  • T to C, chromosome 13 at 113,318,101 bp
  • A to G, chromosome 14 at 55,773,477 bp
  • T to A, chromosome 14 at 56,231,540 bp
  • A to G, chromosome 15 at 39,100,019 bp
  • A to G, chromosome 15 at 101,487,009 bp
  • A to T, chromosome 16 at 19,769,202 bp
  • A to T, chromosome 16 at 96,697,521 bp
  • G to T, chromosome 17 at 57,073,852 bp
  • T to A, chromosome 18 at 37,021,551 bp
  • C to A, chromosome 18 at 43,328,427 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4987 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042581-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.