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Strain Name:
C57BL/6J-MtgxR4989Btlr/Mmmh
Stock Number:
042583-MU
Citation ID:
RRID:MMRRC_042583-MU
Other Names:
R4989 (G1), C57BL/6J-MtgxR4989Btlr
Major Collection:

Strain Information

Lamb1
Name: laminin B1
Synonyms: Lamb-1, C81607, C80098, D130003D08Rik, Lamb1-1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 16777
HGNC: HGNC:6486
Homologene: 1722
Ank2
Name: ankyrin 2, brain
Synonyms: ankyrin B, Ank-2, Ankyrin-2, Ankyrin-B, Gm4392
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 109676
HGNC: HGNC:493
Rprd2
Name: regulation of nuclear pre-mRNA domain containing 2
Synonyms: 6720469I21Rik, 2810036A19Rik, 4930535B03Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 75137
Homologene: 19997
Bri3bp
Name: Bri3 binding protein
Synonyms: 2410150I18Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 76809
Homologene: 50955
Ehmt1
Name: euchromatic histone methyltransferase 1
Synonyms: 9230102N17Rik, KMT1D
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 77683
Homologene: 11698
Prkdc
Name: protein kinase, DNA activated, catalytic polypeptide
Synonyms: DNA-PKcs, slip, DNAPDcs, XRCC7, DNA-PK, DOXNPH, dxnph
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 19090
HGNC: HGNC:9413
Homologene: 5037
Slc37a1
Name: solute carrier family 37 (glycerol-3-phosphate transporter), member 1
Synonyms: G3PP
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 224674
VEGA: 17
Homologene: 70486
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 40,329,001 bp
  • A to G, chromosome 1 at 52,196,210 bp
  • A to T, chromosome 1 at 58,236,676 bp
  • T to C, chromosome 1 at 72,632,860 bp
  • A to G, chromosome 1 at 90,611,002 bp
  • T to C, chromosome 1 at 161,155,306 bp
  • A to G, chromosome 1 at 180,137,801 bp
  • G to T, chromosome 2 at 24,877,497 bp
  • C to T, chromosome 2 at 26,481,181 bp
  • G to T, chromosome 2 at 34,581,291 bp
  • T to C, chromosome 2 at 43,604,258 bp
  • A to T, chromosome 2 at 59,870,414 bp
  • T to C, chromosome 2 at 69,743,724 bp
  • T to C, chromosome 2 at 70,020,446 bp
  • T to A, chromosome 2 at 76,707,242 bp
  • C to T, chromosome 2 at 76,774,778 bp
  • T to A, chromosome 2 at 90,400,763 bp
  • T to A, chromosome 2 at 121,155,549 bp
  • G to A, chromosome 2 at 126,116,123 bp
  • A to T, chromosome 2 at 130,202,041 bp
  • T to C, chromosome 2 at 132,808,271 bp
  • A to G, chromosome 3 at 36,222,136 bp
  • G to A, chromosome 3 at 62,340,385 bp
  • T to G, chromosome 3 at 73,701,844 bp
  • T to A, chromosome 3 at 75,614,488 bp
  • CAGAAG to CAG, chromosome 3 at 89,223,102 bp
  • A to C, chromosome 3 at 95,010,386 bp
  • G to C, chromosome 3 at 95,765,320 bp
  • G to T, chromosome 3 at 95,895,531 bp
  • T to C, chromosome 3 at 108,412,629 bp
  • A to T, chromosome 3 at 126,963,445 bp
  • A to T, chromosome 3 at 131,235,548 bp
  • A to G, chromosome 3 at 146,506,538 bp
  • C to T, chromosome 3 at 154,748,388 bp
  • T to C, chromosome 4 at 44,931,039 bp
  • A to G, chromosome 4 at 46,090,872 bp
  • C to A, chromosome 4 at 48,458,525 bp
  • T to A, chromosome 4 at 57,856,552 bp
  • T to A, chromosome 4 at 108,526,845 bp
  • C to T, chromosome 4 at 130,312,387 bp
  • T to C, chromosome 5 at 3,713,217 bp
  • A to T, chromosome 5 at 3,822,238 bp
  • C to T, chromosome 5 at 24,712,845 bp
  • A to G, chromosome 5 at 96,650,682 bp
  • A to G, chromosome 5 at 108,073,028 bp
  • A to T, chromosome 5 at 109,427,873 bp
  • G to T, chromosome 5 at 125,441,696 bp
  • A to C, chromosome 5 at 134,223,413 bp
  • A to C, chromosome 6 at 57,360,475 bp
  • G to A, chromosome 6 at 86,110,858 bp
  • T to A, chromosome 6 at 137,443,594 bp
  • A to C, chromosome 6 at 147,060,147 bp
  • A to G, chromosome 7 at 17,905,218 bp
  • G to T, chromosome 7 at 24,260,738 bp
  • G to T, chromosome 7 at 25,287,110 bp
  • A to C, chromosome 7 at 34,245,800 bp
  • C to T, chromosome 7 at 45,101,948 bp
  • T to C, chromosome 7 at 73,966,961 bp
  • A to G, chromosome 7 at 103,640,101 bp
  • A to T, chromosome 7 at 106,329,457 bp
  • A to T, chromosome 7 at 118,158,100 bp
  • C to T, chromosome 7 at 118,208,051 bp
  • G to T, chromosome 7 at 127,926,463 bp
  • C to T, chromosome 7 at 135,669,132 bp
  • C to A, chromosome 7 at 140,610,391 bp
  • T to C, chromosome 8 at 13,055,698 bp
  • T to C, chromosome 8 at 23,141,118 bp
  • A to C, chromosome 8 at 27,227,770 bp
  • T to A, chromosome 8 at 46,207,472 bp
  • T to C, chromosome 8 at 68,460,971 bp
  • G to A, chromosome 8 at 79,655,689 bp
  • A to T, chromosome 8 at 110,563,922 bp
  • T to G, chromosome 9 at 37,646,520 bp
  • A to G, chromosome 9 at 38,722,630 bp
  • A to T, chromosome 9 at 44,984,998 bp
  • T to A, chromosome 9 at 107,931,416 bp
  • T to C, chromosome 10 at 80,232,868 bp
  • C to A, chromosome 10 at 112,928,744 bp
  • T to C, chromosome 11 at 9,006,027 bp
  • T to C, chromosome 11 at 23,873,919 bp
  • A to T, chromosome 11 at 97,040,939 bp
  • C to A, chromosome 11 at 102,430,554 bp
  • G to C, chromosome 11 at 103,456,739 bp
  • A to T, chromosome 11 at 106,816,654 bp
  • A to G, chromosome 12 at 31,326,678 bp
  • G to T, chromosome 12 at 71,164,546 bp
  • A to T, chromosome 12 at 71,164,547 bp
  • A to G, chromosome 12 at 84,109,015 bp
  • T to G, chromosome 12 at 113,982,148 bp
  • G to T, chromosome 13 at 22,475,452 bp
  • T to C, chromosome 13 at 27,159,089 bp
  • G to A, chromosome 13 at 38,197,702 bp
  • G to A, chromosome 13 at 48,807,844 bp
  • A to G, chromosome 13 at 52,841,029 bp
  • A to G, chromosome 13 at 53,351,656 bp
  • A to T, chromosome 13 at 54,088,914 bp
  • A to G, chromosome 13 at 61,538,962 bp
  • T to C, chromosome 14 at 50,839,000 bp
  • T to C, chromosome 14 at 55,237,265 bp
  • C to T, chromosome 14 at 67,729,356 bp
  • G to A, chromosome 14 at 73,238,707 bp
  • T to A, chromosome 15 at 12,935,007 bp
  • C to A, chromosome 15 at 99,402,069 bp
  • C to T, chromosome 15 at 102,108,934 bp
  • T to C, chromosome 16 at 3,749,128 bp
  • T to C, chromosome 16 at 15,673,997 bp
  • A to G, chromosome 16 at 45,593,437 bp
  • T to A, chromosome 16 at 50,224,738 bp
  • T to C, chromosome 16 at 59,149,618 bp
  • A to T, chromosome 16 at 87,719,000 bp
  • A to G, chromosome 17 at 31,322,146 bp
  • G to T, chromosome 17 at 37,499,126 bp
  • A to G, chromosome 17 at 40,207,511 bp
  • A to G, chromosome 17 at 86,809,454 bp
  • A to T, chromosome 17 at 90,620,846 bp
  • T to C, chromosome 18 at 12,262,185 bp
  • A to G, chromosome 18 at 84,631,900 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4989 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042583-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.