Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR4991Btlr/Mmmh
Stock Number:
042585-MU
Citation ID:
RRID:MMRRC_042585-MU
Other Names:
R4991 (G1), C57BL/6J-MtgxR4991Btlr
Major Collection:

Strain Information

Ebf2
Name: early B cell factor 2
Synonyms: O/E-3, D14Ggc1e, Mmot1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 13592
Homologene: 56471
Mtmr7
Name: myotubularin related protein 7
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 54384
HGNC: HGNC:7454
Homologene: 99732
Utp20
Name: UTP20 small subunit processome component
Synonyms: mDRIM, DRIM, 3830408P06Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 70683
VEGA: 10
Homologene: 38373
Chmp4b
Name: charged multivesicular body protein 4B
Synonyms: 2010012F05Rik, Snf7-2, chromatin modifying protein 4B
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 75608
Homologene: 57102
Hdac5
Name: histone deacetylase 5
Synonyms: mHDA1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 15184
Homologene: 3995
Nkx2-1
Name: NK2 homeobox 1
Synonyms: thyroid-specific enhancer-binding protein, T/EBP, tinman, Ttf-1, thyroid transcription factor-1, Titf1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 21869
Homologene: 2488
Tpx2
Name: TPX2, microtubule-associated
Synonyms: REPP86, DIL2, p100, 2610005B21Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 72119
HGNC: HGNC:1249
Homologene: 8107
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 14,910,746 bp
  • A to C, chromosome 1 at 34,588,741 bp
  • T to C, chromosome 1 at 59,207,768 bp
  • T to C, chromosome 1 at 93,078,808 bp
  • A to G, chromosome 1 at 174,836,813 bp
  • A to C, chromosome 1 at 178,098,421 bp
  • A to G, chromosome 2 at 85,992,287 bp
  • T to C, chromosome 2 at 152,869,724 bp
  • A to G, chromosome 2 at 154,692,625 bp
  • A to G, chromosome 2 at 172,377,800 bp
  • T to C, chromosome 2 at 177,508,829 bp
  • C to A, chromosome 4 at 145,622,334 bp
  • A to G, chromosome 4 at 149,398,654 bp
  • G to A, chromosome 4 at 149,469,127 bp
  • A to T, chromosome 4 at 149,728,155 bp
  • C to T, chromosome 5 at 30,199,148 bp
  • G to A, chromosome 5 at 30,394,181 bp
  • G to A, chromosome 5 at 92,210,020 bp
  • T to G, chromosome 6 at 8,215,847 bp
  • G to A, chromosome 6 at 47,926,862 bp
  • A to C, chromosome 6 at 69,016,665 bp
  • T to C, chromosome 6 at 85,901,140 bp
  • T to G, chromosome 7 at 3,895,572 bp
  • T to C, chromosome 7 at 4,752,161 bp
  • C to G, chromosome 7 at 19,088,019 bp
  • T to A, chromosome 7 at 44,016,249 bp
  • T to C, chromosome 7 at 75,117,722 bp
  • A to G, chromosome 7 at 85,751,130 bp
  • A to T, chromosome 7 at 141,010,459 bp
  • A to G, chromosome 8 at 40,554,345 bp
  • A to G, chromosome 8 at 119,445,289 bp
  • A to G, chromosome 9 at 35,053,136 bp
  • T to C, chromosome 9 at 86,790,154 bp
  • C to T, chromosome 9 at 110,638,767 bp
  • A to G, chromosome 10 at 41,456,618 bp
  • A to G, chromosome 10 at 76,274,157 bp
  • A to G, chromosome 10 at 80,325,381 bp
  • G to T, chromosome 10 at 88,746,934 bp
  • A to G, chromosome 10 at 103,200,559 bp
  • G to A, chromosome 10 at 117,668,103 bp
  • T to C, chromosome 11 at 58,817,718 bp
  • T to A, chromosome 11 at 102,205,624 bp
  • C to T, chromosome 12 at 21,274,848 bp
  • T to C, chromosome 12 at 56,534,939 bp
  • T to A, chromosome 12 at 89,260,474 bp
  • T to C, chromosome 13 at 13,232,886 bp
  • T to C, chromosome 13 at 20,342,519 bp
  • T to G, chromosome 13 at 23,076,527 bp
  • T to C, chromosome 13 at 23,819,019 bp
  • T to C, chromosome 13 at 59,703,151 bp
  • C to T, chromosome 13 at 62,865,074 bp
  • G to T, chromosome 13 at 73,265,507 bp
  • T to C, chromosome 14 at 26,915,058 bp
  • A to T, chromosome 14 at 47,074,010 bp
  • G to T, chromosome 14 at 55,773,465 bp
  • A to T, chromosome 14 at 67,389,657 bp
  • G to T, chromosome 15 at 48,001,478 bp
  • A to G, chromosome 15 at 59,344,080 bp
  • A to G, chromosome 15 at 99,232,756 bp
  • G to T, chromosome 15 at 102,208,279 bp
  • T to C, chromosome 16 at 96,159,732 bp
  • A to G, chromosome 17 at 36,127,472 bp
  • T to C, chromosome 17 at 37,017,489 bp
  • T to A, chromosome 18 at 5,056,810 bp
  • A to T, chromosome 18 at 36,998,345 bp
  • T to G, chromosome 19 at 12,901,451 bp
  • T to A, chromosome 19 at 31,084,583 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4991 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042585-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.