Strain Name:
B6.129S1-Slc6a1tm1Lst/Mmucd
Stock Number:
000426-UCD
Citation ID:
RRID:MMRRC_000426-UCD
Other Names:
GAT1 knockout/Tac, B6.129S1-Slc6a1tm1Lst/Mmcd

Gene Information

Slc6a1tm1Lst
Name: solute carrier family 6 (neurotransmitter transporter, GABA), member 1; targeted mutation 1, Henry A Lester
Synonyms: GAT-1 KO
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Knockout
Slc6a1
Name: solute carrier family 6 (neurotransmitter transporter, GABA), member 1
Synonyms: XT-1, Gabt, Xtrp1, Gat1, Gabt1, GAT-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Knockout
NCBI: 232333
Homologene: 2290
Genetic Alterations
A floxed neo cassette was introduced into exon 14 of the GABA transporter GAT1 gene (Slc6a1), and this led to near-complete absence of functional expression in the homozygot.
Genotype Determination
ES Cell Line
CJ7 derived from 129S1/Sv-Oca2+ Tyr+ Kitl+
Phenotype
Homozygous: Significant increase in tonic postsynaptic hippocampal GABA(A) receptor-mediated conductance.

Note: After its release from interneurons in the CNS, the major inhibitory neurotransmitter GABA is taken up by GABA transporter (GATs). The predominant neuronal GABA transporter GAT1 is localized in GABAergic axons and nerve terminals, where it is thought to influence GABAergic synaptic transmission. The generation of this GAT1-deficient mice addresses this issue.
Mammalian Phenotype Terms
Allelic Composition: Slc6a1tm1Lst/Slc6a1tm1Lst (Genetic Background: involves: 129S1/Sv * C57BL/6 )

MeSH Terms
  • Animals
  • Carrier Proteins/genetics
  • GABA Plasma Membrane Transport Proteins
  • Hippocampus/metabolism
  • In Vitro Techniques
  • Membrane Proteins/deficiency
  • Membrane Proteins/genetics
  • Membrane Transport Proteins
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Organic Anion Transporters
  • Receptors, GABA-A/genetics
  • Receptors, GABA-A/metabolism
  • Receptors, GABA-B/genetics
  • Receptors, GABA-B/metabolism
  • Synaptic Transmission/physiology
  • Biotinylation
  • Carrier Proteins/biosynthesis
  • Cell Line
  • Cell Membrane/metabolism
  • Cerebellum/cytology
  • Cerebellum/metabolism
  • Cytoplasm/metabolism
  • DNA, Complementary/genetics
  • DNA, Complementary/metabolism
  • Green Fluorescent Proteins
  • Hippocampus/cytology
  • Humans
  • Kidney/cytology
  • Kidney/metabolism
  • Luminescent Proteins/genetics
  • Membrane Proteins/biosynthesis
  • Mice, Transgenic
  • Microscopy, Fluorescence
  • Neurons/cytology
  • Neurons/metabolism
  • Organ Specificity
  • Patch-Clamp Techniques
  • Presynaptic Terminals/metabolism
  • Recombinant Fusion Proteins/biosynthesis
  • Recombinant Fusion Proteins/genetics
  • Stem Cells/cytology
  • gamma-Aminobutyric Acid/metabolism
Strain Development

GAT1-deficient mice were generated as an intermediate in the construction of the mGAT1-GFP strain (PMID:12451126). By homologous recombination, a 2.2-kb neo cassette was inserted into intron 14 of the GAT1-GFP fusion gene in CJ7 embryonic stem cells. Blastocysts were injected with recombinant ES cells and implanted into C57BL/6 females.

Suggested Control Mice
Wild-type littermates
  • Models for Human Disease
  • Neurobiology
Donor
Henry Lester, Ph.D., California Institute of Technology.
Primary Reference

Jensen K, Chiu CS, Sokolova I, Lester HA, Mody I. GABA transporter-1 (GAT1)-deficient mice: differential tonic activation of GABAA versus GABAB receptors in the hippocampus. J Neurophysiol. 2003 Oct;90(4):2690-701. Epub 2003 Jun 18. (Medline PMID: 12815026)

Chiu CS, Jensen K, Sokolova I, Wang D, Li M, Deshpande P, Davidson N, Mody I, Quick MW, Quake SR, Lester HA. Number, density, and surface/cytoplasmic distribution of GABA transporters at presynaptic structures of knock-in mice carrying GABA transporter subtype 1-green fluorescent protein fusions. J Neurosci. 2002 Dec 1;22(23):10251-66. (Medline PMID: 12451126)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu .
Coat Color
Black
Eye
Black
MMRRC Breeding System
Backcross
Breeding Scheme(s)
C57BL/6 Female x Heterozygous Male
Generation
N? (C57BL/6)
Overall Breeding Performance
Homozygotes are poor breeders
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Unknown Unknown
Bred to Homozygosity
Yes
Average litter size
6 - 8
Recommended wean age
3 weeks
Average Pups Weaned
6 - 8

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Meimei Dong.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Meimei Dong

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
000426-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / $1,545.65
Non-Profit / For-Profit
Aliquot Approximate quantity2 : 20-40 embryos / aliquot
000426-UCD-SPERM Cryo-preserved spermatozoa $546.25 / $869.88
Non-Profit / For-Profit
Aliquot Approximate quantity3
000426-UCD-RESUS Litter recovered from cryo-archive $3,033.00 / $7,533.58
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.