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Strain Name:
C57BL/6J-MtgxR5014Btlr/Mmmh
Stock Number:
042605-MU
Citation ID:
RRID:MMRRC_042605-MU
Other Names:
R5014 (G1), C57BL/6J-MtgxR5014Btlr
Major Collection:

Strain Information

Vcp
Name: valosin containing protein
Synonyms: CDC48, p97, p97/VCP, AAA ATPase p97
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 269523
Homologene: 5168
Rcan2
Name: regulator of calcineurin 2
Synonyms: MCIP2, ZAKI-4, Csp2, Dscr1l1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 53901
VEGA: 17
HGNC: HGNC:3041
Homologene: 130985
Ints6
Name: integrator complex subunit 6
Synonyms: Notch2l, 2900075H24Rik, DICE1, Ddx26
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 18130
VEGA: 14
Homologene: 8121
Rbx1
Name: ring-box 1
Synonyms: 1500002P15Rik, ROC1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 56438
VEGA: 15
HGNC: HGNC:9928
Homologene: 134530
Col18a1
Name: collagen, type XVIII, alpha 1
Synonyms: endostatin
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 12822
HGNC: HGNC:2195
Homologene: 7673
Dnmt1
Name: DNA methyltransferase 1
Synonyms: MTase, Dnmt1o, Cxxc9, MommeD2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 13433
VEGA: 9
HGNC: HGNC:2976
Homologene: 124071
Ylpm1
Name: YLP motif containing 1
Synonyms: ZAP, Zap3, A930013E17Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 56531
Homologene: 87707
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 4,910,547 bp
  • T to C, chromosome 1 at 21,293,597 bp
  • C to A, chromosome 1 at 57,406,845 bp
  • T to A, chromosome 1 at 58,591,242 bp
  • T to C, chromosome 1 at 80,200,059 bp
  • A to C, chromosome 1 at 85,577,329 bp
  • A to T, chromosome 1 at 132,584,447 bp
  • T to C, chromosome 1 at 155,928,206 bp
  • T to C, chromosome 1 at 172,284,871 bp
  • A to G, chromosome 1 at 192,277,080 bp
  • T to C, chromosome 2 at 32,936,395 bp
  • T to A, chromosome 2 at 37,487,140 bp
  • G to A, chromosome 2 at 69,881,380 bp
  • T to A, chromosome 2 at 118,120,037 bp
  • G to A, chromosome 2 at 126,116,123 bp
  • G to A, chromosome 2 at 158,495,535 bp
  • T to C, chromosome 2 at 160,904,828 bp
  • C to T, chromosome 2 at 163,087,192 bp
  • C to G, chromosome 3 at 82,046,667 bp
  • T to G, chromosome 3 at 94,391,153 bp
  • A to C, chromosome 3 at 101,003,823 bp
  • T to C, chromosome 4 at 42,980,828 bp
  • C to T, chromosome 4 at 58,951,892 bp
  • T to C, chromosome 4 at 108,526,846 bp
  • C to T, chromosome 4 at 109,235,797 bp
  • T to G, chromosome 4 at 119,393,627 bp
  • T to A, chromosome 4 at 145,538,099 bp
  • G to T, chromosome 5 at 76,633,702 bp
  • T to C, chromosome 5 at 123,617,730 bp
  • C to T, chromosome 6 at 29,326,724 bp
  • T to C, chromosome 6 at 82,743,955 bp
  • T to A, chromosome 6 at 124,855,236 bp
  • C to T, chromosome 6 at 128,200,593 bp
  • T to C, chromosome 6 at 128,543,933 bp
  • T to A, chromosome 6 at 128,948,802 bp
  • T to C, chromosome 6 at 145,183,266 bp
  • G to T, chromosome 7 at 24,260,738 bp
  • A to G, chromosome 7 at 139,627,375 bp
  • C to T, chromosome 8 at 22,625,299 bp
  • T to C, chromosome 8 at 57,545,380 bp
  • A to G, chromosome 8 at 84,991,377 bp
  • A to C, chromosome 9 at 20,912,254 bp
  • C to T, chromosome 9 at 42,373,242 bp
  • A to G, chromosome 9 at 120,422,468 bp
  • A to T, chromosome 10 at 58,464,120 bp
  • C to T, chromosome 10 at 77,070,960 bp
  • T to A, chromosome 10 at 106,865,363 bp
  • T to C, chromosome 11 at 45,991,909 bp
  • T to A, chromosome 11 at 46,344,833 bp
  • T to A, chromosome 11 at 69,665,339 bp
  • T to A, chromosome 11 at 69,761,040 bp
  • T to A, chromosome 11 at 77,455,276 bp
  • T to A, chromosome 11 at 77,498,995 bp
  • T to C, chromosome 11 at 78,400,161 bp
  • A to T, chromosome 11 at 109,950,131 bp
  • G to A, chromosome 12 at 85,014,749 bp
  • A to G, chromosome 13 at 21,958,424 bp
  • A to T, chromosome 14 at 33,100,940 bp
  • A to T, chromosome 14 at 62,760,191 bp
  • T to G, chromosome 14 at 63,379,787 bp
  • T to A, chromosome 15 at 81,470,960 bp
  • T to C, chromosome 15 at 98,456,976 bp
  • C to T, chromosome 15 at 102,108,934 bp
  • T to A, chromosome 16 at 3,839,048 bp
  • C to A, chromosome 16 at 4,039,016 bp
  • T to A, chromosome 16 at 36,721,559 bp
  • T to A, chromosome 16 at 59,666,579 bp
  • G to A, chromosome 17 at 20,958,839 bp
  • G to A, chromosome 17 at 30,748,568 bp
  • A to T, chromosome 17 at 32,198,398 bp
  • T to C, chromosome 17 at 32,359,366 bp
  • A to G, chromosome 17 at 34,847,425 bp
  • T to A, chromosome 17 at 37,085,554 bp
  • T to C, chromosome 17 at 44,017,813 bp
  • A to G, chromosome 17 at 46,655,942 bp
  • A to T, chromosome 17 at 87,717,576 bp
  • T to G, chromosome 19 at 4,143,507 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5014 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042605-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.