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Strain Name:
C57BL/6J-MtgxR5014Btlr/Mmmh
Stock Number:
042605-MU
Citation ID:
RRID:MMRRC_042605-MU
Other Names:
R5014 (G1), C57BL/6J-MtgxR5014Btlr
Major Collection:

Strain Information

Vcp
Name: valosin containing protein
Synonyms: CDC48, p97, p97/VCP, AAA ATPase p97
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 269523
Homologene: 5168
Rcan2
Name: regulator of calcineurin 2
Synonyms: MCIP2, ZAKI-4, Csp2, Dscr1l1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 53901
VEGA: 17
HGNC: HGNC:3041
Homologene: 130985
Ints6
Name: integrator complex subunit 6
Synonyms: Notch2l, 2900075H24Rik, DICE1, Ddx26
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 18130
VEGA: 14
Homologene: 8121
Rbx1
Name: ring-box 1
Synonyms: 1500002P15Rik, ROC1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 56438
VEGA: 15
HGNC: HGNC:9928
Homologene: 134530
Col18a1
Name: collagen, type XVIII, alpha 1
Synonyms: endostatin
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 12822
HGNC: HGNC:2195
Homologene: 7673
Dnmt1
Name: DNA methyltransferase 1
Synonyms: MTase, Dnmt1o, Cxxc9, MommeD2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 13433
VEGA: 9
HGNC: HGNC:2976
Homologene: 124071
Ylpm1
Name: YLP motif containing 1
Synonyms: ZAP, Zap3, A930013E17Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 56531
Homologene: 87707
Lrsam1
Name: leucine rich repeat and sterile alpha motif containing 1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227738
Homologene: 44526
Tyw5
Name: tRNA-yW synthesizing protein 5
Synonyms: 1110034B05Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 68736
Homologene: 72085
Brd4
Name: bromodomain containing 4
Synonyms: MCAP, HUNK1, WI-11513
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 57261
Homologene: 136213
Msh2
Name: mutS homolog 2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 17685
HGNC: HGNC:7325
Homologene: 210
Ralgapb
Name: Ral GTPase activating protein, beta subunit (non-catalytic)
Synonyms: B230339M05Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228850
Homologene: 10666
Tut4
Name: terminal uridylyl transferase 4
Synonyms: 6030404K05Rik, 9230115F04Rik, Zcchc11, Tent3a
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230594
Homologene: 35279
Rabgap1
Name: RAB GTPase activating protein 1
Synonyms: Gapcena
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227800
Homologene: 49301
Cep350
Name: centrosomal protein 350
Synonyms: 6430546F08Rik, 4933409L06Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 74081
Homologene: 8879
Git1
Name: GIT ArfGAP 1
Synonyms: p95Cat, Cat-1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216963
HGNC: HGNC:4272
Homologene: 32204
Ppp2r1a
Name: protein phosphatase 2, regulatory subunit A, alpha
Synonyms: PR65, protein phosphatase PP2A, 6330556D22Rik, PP2A
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 51792
HGNC: HGNC:9302
Homologene: 68559
Hk2
Name: hexokinase 2
Synonyms: HKII
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 15277
HGNC: HGNC:4923
Homologene: 37273
Cep135
Name: centrosomal protein 135
Synonyms: LOC381644, Cep4
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 381644
Homologene: 45709
Thbs1
Name: thrombospondin 1
Synonyms: TSP1, TSP-1, Thbs-1, tbsp1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 21825
Homologene: 31142
Cul7
Name: cullin 7
Synonyms: p193, 2510004L20Rik, p185, C230011P08Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 66515
Homologene: 56683
Epha6
Name: Eph receptor A6
Synonyms: m-ehk2, Ehk2, Hek12
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 13840
Homologene: 56396
Dnah8
Name: dynein, axonemal, heavy chain 8
Synonyms: Hst6.7b, P1-Loop, Dnahc8
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 13417
VEGA: 17
HGNC: HGNC:2952
Homologene: 1049
Gtsf1l
Name: gametocyte specific factor 1-like
Synonyms: 2410116G06Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 68236
Homologene: 32667
Ssh2
Name: slingshot protein phosphatase 2
Synonyms: SSH-2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 237860
Homologene: 14116
Cd68
Name: CD68 antigen
Synonyms: macrosialin, gp110, Scard1, Lamp4
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 12514
HGNC: HGNC:1693
Homologene: 955
Skic2
Name: SKI2 subunit of superkiller complex
Synonyms: Ski2w, 4930534J06Rik, Skiv2l
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 108077
Homologene: 123971
Gpr152
Name: G protein-coupled receptor 152
Synonyms: LOC269053, A930009H15Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 269053
VEGA: 19
Homologene: 35474
Rorc
Name: RAR-related orphan receptor gamma
Synonyms: thymus orphan receptor, Thor, RORgamma
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 19885
Homologene: 21051
Cfap46
Name: cilia and flagella associated protein 46
Synonyms: 9330101J02Rik, Ttc40
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 212124
Tns2
Name: tensin 2
Synonyms: nep, nph, Tenc1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 209039
VEGA: 15
Homologene: 37077
P3h3
Name: prolyl 3-hydroxylase 3
Synonyms: Grcb, Leprel2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 14789
Homologene: 8401
Tecta
Name: tectorin alpha
Synonyms: [a]-tectorin, Tctna
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 21683
Homologene: 3955
Zfp990
Name: zinc finger protein 990
Synonyms: Gm13225
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 101056073
Dnase1
Name: deoxyribonuclease I
Synonyms: DNaseI, Dnl1
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 13419
HGNC: HGNC:2956
Homologene: 3826
Dnai7
Name: dynein axonemal intermediate chain 7
Synonyms: A230084G12Rik, Las1, Casc1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 320662
Homologene: 41242
Gucy1b1
Name: guanylate cyclase 1, soluble, beta 1
Synonyms: beta 1 sGC, Gucy1b3
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 54195
HGNC: HGNC:4687
Homologene: 664
Wdfy4
Name: WD repeat and FYVE domain containing 4
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 545030
Homologene: 83473
Ppfia2
Name: protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 2
Synonyms: E130120L08Rik, Liprin-alpha2
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 327814
VEGA: 10
HGNC: HGNC:9246
Homologene: 27953
A2ml1
Name: alpha-2-macroglobulin like 1
Synonyms: Ovos2, BC048546
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232400
Homologene: 45969
Abca8b
Name: ATP-binding cassette, sub-family A member 8b
Synonyms: Abca8
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 27404
HGNC: HGNC:38
Homologene: 56029
Cd101
Name: CD101 antigen
Synonyms: LOC381460, Igsf2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 630146
HGNC: HGNC:5949
Homologene: 3142
Clec2g
Name: C-type lectin domain family 2, member g
Synonyms: 4632413B12Rik, Ocilrp1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 70809
Homologene: 136309
Fam227b
Name: family with sequence similarity 227, member B
Synonyms: 4930525F21Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 75823
Homologene: 27384
Slc13a2
Name: solute carrier family 13 (sodium-dependent dicarboxylate transporter), member 2
Synonyms: sodium/dicarboxylate co-transporter, mNaDC-1, Nadc1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20500
Homologene: 2965
Lpin3
Name: lipin 3
Synonyms: 9130206L11Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 64899
Homologene: 84844
Sox30
Name: SRY (sex determining region Y)-box 30
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 214105
Homologene: 34027
Atp1a2
Name: ATPase, Na+/K+ transporting, alpha 2 polypeptide
Synonyms: Atpa-3
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 98660
HGNC: HGNC:800
Homologene: 47947
Zfp108
Name: zinc finger protein 108
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 54678
Homologene: 117959
Ccdc30
Name: coiled-coil domain containing 30
Synonyms: 1700111D19Rik, 4930445I03Rik, 1700001O02Rik, 1700041C02Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 73332
Homologene: 137391
Ildr1
Name: immunoglobulin-like domain containing receptor 1
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 106347
Homologene: 15892
Wiz
Name: widely-interspaced zinc finger motifs
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 22404
Homologene: 7997
Kcnh1
Name: potassium voltage-gated channel, subfamily H (eag-related), member 1
Synonyms: ether a go-go, Eag1, Kv10.1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 16510
HGNC: HGNC:6250
Homologene: 68242
Fam124b
Name: family with sequence similarity 124, member B
Synonyms: A830043J08Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 241128
Homologene: 65263
Clip1
Name: CAP-GLY domain containing linker protein 1
Synonyms: cytoplasmic linker protein 50, Clip50, CLIP-170, 1110007I12Rik, 4631429H07Rik, Rsn, Clip 170, restin
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 56430
Homologene: 74455
Blk
Name: B lymphoid kinase
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 12143
VEGA: 14
HGNC: HGNC:1057
Homologene: 1297
Zkscan16
Name: zinc finger with KRAB and SCAN domains 16
Synonyms: Zfp483
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 100041581
Homologene: 26419
Or2c1
Name: olfactory receptor family 2 subfamily C member 1
Synonyms: OR3, GA_x54KRFPKG5P-348087-349025, MOR256-17, Olfr15
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 18312
HGNC: HGNC:8242
Homologene: 7459
Zfp184
Name: zinc finger protein 184 (Kruppel-like)
Synonyms: 4930500C15Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 193452
Homologene: 113602
Hook2
Name: hook microtubule tethering protein 2
Synonyms: A630054I03Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 170833
Homologene: 8332
Galnt7
Name: polypeptide N-acetylgalactosaminyltransferase 7
Synonyms: ppGaNTase-T7
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 108150
HGNC: HGNC:4129
Homologene: 9685
Garin1b
Name: golgi associated RAB2 interactor 1B
Synonyms: LOC330277, Fam71f1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 330277
Homologene: 13077
Sp110
Name: Sp110 nuclear body protein
Synonyms: 52kDa, Ipr1, 5031415C07Rik, 5830484A20Rik, Ifi75
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 109032
HGNC: HGNC:5401
Homologene: 82192
Myrip
Name: myosin VIIA and Rab interacting protein
Synonyms: Slac2-c, A230081N12Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 245049
Homologene: 9150
Dkk4
Name: dickkopf WNT signaling pathway inhibitor 4
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234130
HGNC: HGNC:2894
Homologene: 8680
Calr4
Name: calreticulin 4
Synonyms: 4933403L16Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 108802
Homologene: 86813
Rgs20
Name: regulator of G-protein signaling 20
Synonyms: Rgsz1, 2900073E09Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 58175
Homologene: 2745
Gm10010
Name: predicted gene 10010
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Or8s8
Name: olfactory receptor family 8 subfamily S member 8
Synonyms: GA_x6K02T2NBG7-5275017-5274082, MOR160-5, Olfr281
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 258277
Homologene: 133703
Mettl5
Name: methyltransferase 5, N6-adenosine
Synonyms: 2810410A08Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 75422
Homologene: 8570
Slc35g3
Name: solute carrier family 35, member G3
Synonyms: Amac1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 56293
Homologene: 89413
Or2h2
Name: olfactory receptor family 2 subfamily H member 2
Synonyms: MOR256-21, GA_x6K02T2PSCP-1526227-1525295, Olfr90
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 258469
HGNC: HGNC:8253
Homologene: 68546
Gm4956
Name: predicted gene 4956
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 241041
Ndufb3
Name: NADH:ubiquinone oxidoreductase subunit B3
Synonyms: 2700033I16Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 66495
HGNC: HGNC:7698
Homologene: 37630
Gm12167
Name: predicted gene 12167
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 100503965
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 4,910,547 bp
  • T to C, chromosome 1 at 21,293,597 bp
  • C to A, chromosome 1 at 57,406,845 bp
  • T to A, chromosome 1 at 58,591,242 bp
  • T to C, chromosome 1 at 80,200,059 bp
  • A to C, chromosome 1 at 85,577,329 bp
  • A to T, chromosome 1 at 132,584,447 bp
  • T to C, chromosome 1 at 155,928,206 bp
  • T to C, chromosome 1 at 172,284,871 bp
  • A to G, chromosome 1 at 192,277,080 bp
  • T to C, chromosome 2 at 32,936,395 bp
  • T to A, chromosome 2 at 37,487,140 bp
  • G to A, chromosome 2 at 69,881,380 bp
  • T to A, chromosome 2 at 118,120,037 bp
  • G to A, chromosome 2 at 126,116,123 bp
  • G to A, chromosome 2 at 158,495,535 bp
  • T to C, chromosome 2 at 160,904,828 bp
  • C to T, chromosome 2 at 163,087,192 bp
  • C to G, chromosome 3 at 82,046,667 bp
  • T to G, chromosome 3 at 94,391,153 bp
  • A to C, chromosome 3 at 101,003,823 bp
  • T to C, chromosome 4 at 42,980,828 bp
  • C to T, chromosome 4 at 58,951,892 bp
  • T to C, chromosome 4 at 108,526,846 bp
  • C to T, chromosome 4 at 109,235,797 bp
  • T to G, chromosome 4 at 119,393,627 bp
  • T to A, chromosome 4 at 145,538,099 bp
  • G to T, chromosome 5 at 76,633,702 bp
  • T to C, chromosome 5 at 123,617,730 bp
  • C to T, chromosome 6 at 29,326,724 bp
  • T to C, chromosome 6 at 82,743,955 bp
  • T to A, chromosome 6 at 124,855,236 bp
  • C to T, chromosome 6 at 128,200,593 bp
  • T to C, chromosome 6 at 128,543,933 bp
  • T to A, chromosome 6 at 128,948,802 bp
  • T to C, chromosome 6 at 145,183,266 bp
  • G to T, chromosome 7 at 24,260,738 bp
  • A to G, chromosome 7 at 139,627,375 bp
  • C to T, chromosome 8 at 22,625,299 bp
  • T to C, chromosome 8 at 57,545,380 bp
  • A to G, chromosome 8 at 84,991,377 bp
  • A to C, chromosome 9 at 20,912,254 bp
  • C to T, chromosome 9 at 42,373,242 bp
  • A to G, chromosome 9 at 120,422,468 bp
  • A to T, chromosome 10 at 58,464,120 bp
  • C to T, chromosome 10 at 77,070,960 bp
  • T to A, chromosome 10 at 106,865,363 bp
  • T to C, chromosome 11 at 45,991,909 bp
  • T to A, chromosome 11 at 46,344,833 bp
  • T to A, chromosome 11 at 69,665,339 bp
  • T to A, chromosome 11 at 69,761,040 bp
  • T to A, chromosome 11 at 77,455,276 bp
  • T to A, chromosome 11 at 77,498,995 bp
  • T to C, chromosome 11 at 78,400,161 bp
  • A to T, chromosome 11 at 109,950,131 bp
  • G to A, chromosome 12 at 85,014,749 bp
  • A to G, chromosome 13 at 21,958,424 bp
  • A to T, chromosome 14 at 33,100,940 bp
  • A to T, chromosome 14 at 62,760,191 bp
  • T to G, chromosome 14 at 63,379,787 bp
  • T to A, chromosome 15 at 81,470,960 bp
  • T to C, chromosome 15 at 98,456,976 bp
  • C to T, chromosome 15 at 102,108,934 bp
  • T to A, chromosome 16 at 3,839,048 bp
  • C to A, chromosome 16 at 4,039,016 bp
  • T to A, chromosome 16 at 36,721,559 bp
  • T to A, chromosome 16 at 59,666,579 bp
  • G to A, chromosome 17 at 20,958,839 bp
  • G to A, chromosome 17 at 30,748,568 bp
  • A to T, chromosome 17 at 32,198,398 bp
  • T to C, chromosome 17 at 32,359,366 bp
  • A to G, chromosome 17 at 34,847,425 bp
  • T to A, chromosome 17 at 37,085,554 bp
  • T to C, chromosome 17 at 44,017,813 bp
  • A to G, chromosome 17 at 46,655,942 bp
  • A to T, chromosome 17 at 87,717,576 bp
  • T to G, chromosome 19 at 4,143,507 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5014 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042605-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.