Strain Name:
C57BL/6J-MtgxR5025Btlr/Mmmh
Stock Number:
042616-MU
Citation ID:
RRID:MMRRC_042616-MU
Other Names:
R5025 (G1), C57BL/6J-MtgxR5025Btlr
Major Collection:

Gene Information

Camsap3
Name: calmodulin regulated spectrin-associated protein family, member 3
Synonyms: Nezha, 2310057J16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 69697
Homologene: 18966
Zdbf2
Name: zinc finger, DBF-type containing 2
Synonyms: 9330107J05Rik, 4930431J08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 73884
Homologene: 52868
Wdr47
Name: WD repeat domain 47
Synonyms: 1810073M12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 99512
Homologene: 8984
Atg14
Name: autophagy related 14
Synonyms: Barkor, D14Ertd114e, D14Ertd436e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 100504663
VEGA: 14
Homologene: 45423
Fbrsl1
Name: fibrosin-like 1
Synonyms: LOC381668, Gm29766, 2410025L10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 381668
Homologene: 45966
Cad
Name: carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase
Synonyms: 2410008J01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 69719
HGNC: HGNC:1424
Homologene: 1412
Brwd1
Name: bromodomain and WD repeat domain containing 1
Synonyms: Wdr9, D530019K20Rik, 5330419I02Rik, G1-403-16, repro5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 93871
Homologene: 23130
Dennd4c
Name: DENN/MADD domain containing 4C
Synonyms: 1700065A05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 329877
Homologene: 23057
Fasn
Name: fatty acid synthase
Synonyms: A630082H08Rik, FAS
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 14104
HGNC: HGNC:3594
Homologene: 55800
Herc1
Name: HECT and RLD domain containing E3 ubiquitin protein ligase family member 1
Synonyms: D130015N03Rik, 2810449H11Rik, tbl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 235439
HGNC: HGNC:4867
Homologene: 31207
Tg
Name: thyroglobulin
Synonyms: Tgn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 21819
Homologene: 2430
Gpsm1
Name: G-protein signalling modulator 1 (AGS3-like, C. elegans)
Synonyms: Ags3, 1810037C22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 67839
Homologene: 16987
Fbxl18
Name: F-box and leucine-rich repeat protein 18
Synonyms: C330021B20Rik, B130019G13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 231863
Homologene: 41603
Mrpl15
Name: mitochondrial ribosomal protein L15
Synonyms: MRP-L7, HSPC145, Rpml7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 27395
Homologene: 32210
Inpp5j
Name: inositol polyphosphate 5-phosphatase J
Synonyms: Pib5pa, Pipp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 170835
HGNC: HGNC:8956
Homologene: 8682
Il17rc
Name: interleukin 17 receptor C
Synonyms: Il17rl, 1110025H02Rik, IL17-RL
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 171095
Homologene: 15894
Rnf219
Name: ring finger protein 219
Synonyms: 2610206B13Rik, 2810449K13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 72486
VEGA: 14
Homologene: 11578
Brip1
Name: BRCA1 interacting protein C-terminal helicase 1
Synonyms: 3110009N10Rik, 8030460J03Rik, BACH1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 237911
Homologene: 32766
Tlr3
Name: toll-like receptor 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 142980
Homologene: 20696
Lamc3
Name: laminin gamma 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 23928
HGNC: HGNC:6494
Homologene: 21222
Olfr1484
Name: olfactory receptor 1484
Synonyms: MOR202-37, GA_x6K02T2RE5P-3917859-3918806
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 258288
Homologene: 110492
Dnah3
Name: dynein, axonemal, heavy chain 3
Synonyms: Dnahc3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 381917
HGNC: HGNC:2949
Homologene: 19674
Myh7b
Name: myosin, heavy chain 7B, cardiac muscle, beta
Synonyms: Myh14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 668940
Homologene: 66117
Adad1
Name: adenosine deaminase domain containing 1 (testis specific)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 21744
Homologene: 7569
Eef1akmt2
Name: EEF1A lysine methyltransferase 2
Synonyms: Mettl10, 2010208K18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 72096
Homologene: 6175
Tns1
Name: tensin 1
Synonyms: 1200014E20Rik, E030037J05Rik, 1110018I21Rik, Tns, E030018G17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 21961
Homologene: 11219
Tnfsf15
Name: tumor necrosis factor (ligand) superfamily, member 15
Synonyms: TL1, TL1A, VEGI
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 326623
Homologene: 3755
Olfr135
Name: olfactory receptor 135
Synonyms: GA_x6K02T2PSCP-2656648-2657586, MOR256-48
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 258329
Homologene: 110603
Hddc2
Name: HD domain containing 2
Synonyms: 2310057G13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 69692
Homologene: 5431
Rimbp3
Name: RIMS binding protein 3
Synonyms: RIM-BP3, LOC385766, LOC239731
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 239731
Homologene: 77940
Fut9
Name: fucosyltransferase 9
Synonyms: mFuc-TIX, mFUT9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 14348
HGNC: HGNC:4020
Homologene: 4800
Zc3h6
Name: zinc finger CCCH type containing 6
Synonyms: 4833425H18Rik, 4631426G04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 78751
Homologene: 35328
Acsm2
Name: acyl-CoA synthetase medium-chain family member 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 233799
Homologene: 70404
Fuca1
Name: fucosidase, alpha-L- 1, tissue
Synonyms: Afuc, 0610006A03Rik, 9530055J05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 71665
HGNC: HGNC:4006
Homologene: 20078
Snai2
Name: snail family zinc finger 2
Synonyms: Slug, Slugh, Snail2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 20583
VEGA: 16
Homologene: 31127
Glra1
Name: glycine receptor, alpha 1 subunit
Synonyms: oscillator, nmf11, ot, B230397M16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 14654
HGNC: HGNC:4326
Homologene: 20083
Hadha
Name: hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit
Synonyms: Mtpa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 97212
HGNC: HGNC:4801
Homologene: 152
Igkv14-100
Name: immunoglobulin kappa chain variable 14-100
Synonyms: Gm16635
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 243439
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 4,784,145 bp
  • A to G, chromosome 1 at 63,303,650 bp
  • G to A, chromosome 1 at 73,925,482 bp
  • T to C, chromosome 2 at 26,319,996 bp
  • T to A, chromosome 2 at 31,908,669 bp
  • T to A, chromosome 2 at 129,010,433 bp
  • G to C, chromosome 2 at 155,632,373 bp
  • G to T, chromosome 3 at 37,065,210 bp
  • A to G, chromosome 3 at 108,618,573 bp
  • T to A, chromosome 4 at 25,620,502 bp
  • T to C, chromosome 4 at 63,729,888 bp
  • T to A, chromosome 4 at 86,795,299 bp
  • G to T, chromosome 4 at 135,932,926 bp
  • A to G, chromosome 5 at 30,154,961 bp
  • G to T, chromosome 5 at 31,072,373 bp
  • T to C, chromosome 5 at 110,417,901 bp
  • A to T, chromosome 5 at 142,886,313 bp
  • A to G, chromosome 6 at 68,519,399 bp
  • T to C, chromosome 6 at 113,472,366 bp
  • T to A, chromosome 7 at 119,554,496 bp
  • T to C, chromosome 7 at 120,071,905 bp
  • C to A, chromosome 7 at 132,851,489 bp
  • A to G, chromosome 8 at 3,604,244 bp
  • A to G, chromosome 8 at 45,403,038 bp
  • A to T, chromosome 9 at 66,470,326 bp
  • C to T, chromosome 10 at 31,327,953 bp
  • T to C, chromosome 11 at 3,500,664 bp
  • C to T, chromosome 11 at 55,536,505 bp
  • T to A, chromosome 11 at 86,064,980 bp
  • A to T, chromosome 11 at 120,811,908 bp
  • T to G, chromosome 14 at 47,545,816 bp
  • C to T, chromosome 14 at 104,508,030 bp
  • A to T, chromosome 15 at 66,707,930 bp
  • A to G, chromosome 16 at 14,708,189 bp
  • A to G, chromosome 16 at 17,209,807 bp
  • A to G, chromosome 16 at 96,053,972 bp
  • T to C, chromosome 17 at 38,208,443 bp
  • G to T, chromosome 19 at 13,585,522 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5025 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042616-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.