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Strain Name:
C57BL/6J-MtgxR5033Btlr/Mmmh
Stock Number:
042624-MU
Citation ID:
RRID:MMRRC_042624-MU
Other Names:
R5033 (G1), C57BL/6J-MtgxR5033Btlr
Major Collection:

Strain Information

Sulf2
Name: sulfatase 2
Synonyms: 2010004N24Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 72043
Homologene: 10313
Arhgap10
Name: Rho GTPase activating protein 10
Synonyms: PSGAP-s, PSGAP-m, A930033B01Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 78514
Homologene: 12695
Kmt2c
Name: lysine (K)-specific methyltransferase 2C
Synonyms: E330008K23Rik, HALR, Mll3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231051
Homologene: 46480
Col3a1
Name: collagen, type III, alpha 1
Synonyms: Col3a-1, Tsk-2, Tsk2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12825
HGNC: HGNC:2201
Homologene: 55433
Lars1
Name: leucyl-tRNA synthetase 1
Synonyms: 3110009L02Rik, 2310045K21Rik, Lars
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 107045
VEGA: 18
HGNC: HGNC:6512
Homologene: 7083
Prpf6
Name: pre-mRNA splicing factor 6
Synonyms: 1190003A07Rik, U5-102K, ANT-1, 2610031L17Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 68879
Homologene: 5368
Xpnpep1
Name: X-prolyl aminopeptidase (aminopeptidase P) 1, soluble
Synonyms: D230045I08Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 170750
Homologene: 6424
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 40,007,502 bp
  • T to G, chromosome 1 at 40,293,524 bp
  • T to A, chromosome 1 at 42,895,331 bp
  • A to C, chromosome 1 at 45,346,110 bp
  • A to G, chromosome 1 at 88,205,023 bp
  • G to A, chromosome 1 at 92,857,029 bp
  • A to T, chromosome 1 at 130,844,699 bp
  • T to C, chromosome 1 at 171,222,043 bp
  • T to A, chromosome 1 at 180,065,015 bp
  • A to G, chromosome 1 at 185,317,928 bp
  • C to T, chromosome 1 at 188,996,506 bp
  • A to G, chromosome 2 at 23,194,747 bp
  • T to C, chromosome 2 at 24,087,158 bp
  • A to G, chromosome 2 at 26,419,649 bp
  • T to A, chromosome 2 at 76,886,442 bp
  • T to A, chromosome 2 at 87,607,711 bp
  • T to A, chromosome 2 at 91,054,608 bp
  • T to A, chromosome 2 at 120,693,399 bp
  • C to T, chromosome 2 at 120,911,997 bp
  • T to A, chromosome 2 at 122,522,914 bp
  • T to A, chromosome 2 at 166,081,622 bp
  • T to A, chromosome 2 at 181,650,106 bp
  • T to A, chromosome 3 at 64,105,080 bp
  • A to G, chromosome 3 at 64,259,799 bp
  • A to G, chromosome 3 at 121,156,275 bp
  • A to T, chromosome 4 at 43,019,412 bp
  • A to T, chromosome 4 at 55,530,301 bp
  • T to C, chromosome 4 at 107,834,755 bp
  • A to G, chromosome 4 at 117,052,736 bp
  • A to G, chromosome 4 at 124,658,656 bp
  • A to G, chromosome 4 at 128,586,128 bp
  • C to T, chromosome 4 at 130,221,615 bp
  • C to T, chromosome 4 at 137,518,940 bp
  • A to G, chromosome 4 at 139,371,696 bp
  • A to G, chromosome 4 at 141,000,821 bp
  • T to C, chromosome 4 at 141,467,099 bp
  • A to C, chromosome 4 at 152,313,209 bp
  • A to T, chromosome 4 at 155,648,825 bp
  • G to A, chromosome 5 at 25,314,708 bp
  • G to A, chromosome 5 at 30,929,660 bp
  • A to G, chromosome 5 at 38,320,201 bp
  • C to T, chromosome 5 at 98,185,212 bp
  • C to A, chromosome 5 at 108,650,315 bp
  • G to A, chromosome 5 at 114,634,669 bp
  • A to G, chromosome 5 at 114,635,597 bp
  • A to T, chromosome 5 at 124,543,058 bp
  • A to T, chromosome 6 at 41,699,055 bp
  • A to G, chromosome 6 at 52,777,540 bp
  • T to A, chromosome 6 at 137,443,594 bp
  • C to T, chromosome 7 at 27,636,422 bp
  • A to C, chromosome 7 at 29,710,134 bp
  • C to T, chromosome 7 at 34,245,812 bp
  • A to G, chromosome 7 at 45,154,854 bp
  • G to T, chromosome 7 at 99,660,049 bp
  • A to G, chromosome 7 at 119,454,291 bp
  • C to A, chromosome 7 at 139,603,860 bp
  • A to T, chromosome 8 at 12,430,676 bp
  • A to T, chromosome 8 at 19,691,678 bp
  • A to T, chromosome 8 at 77,382,757 bp
  • A to G, chromosome 8 at 88,806,691 bp
  • C to T, chromosome 9 at 38,349,628 bp
  • A to G, chromosome 9 at 103,279,414 bp
  • AGCCG to AG, chromosome 9 at 108,611,660 bp
  • A to T, chromosome 9 at 110,980,919 bp
  • C to T, chromosome 9 at 123,586,399 bp
  • C to T, chromosome 10 at 52,128,416 bp
  • A to G, chromosome 10 at 58,278,806 bp
  • A to T, chromosome 10 at 76,399,945 bp
  • A to G, chromosome 10 at 81,305,250 bp
  • A to G, chromosome 10 at 115,359,761 bp
  • A to T, chromosome 11 at 5,129,079 bp
  • T to C, chromosome 11 at 32,195,044 bp
  • G to A, chromosome 11 at 48,947,706 bp
  • A to T, chromosome 11 at 54,691,381 bp
  • A to T, chromosome 11 at 83,186,797 bp
  • A to T, chromosome 11 at 96,050,322 bp
  • A to T, chromosome 11 at 98,682,824 bp
  • G to T, chromosome 11 at 109,794,154 bp
  • A to T, chromosome 12 at 32,199,196 bp
  • A to T, chromosome 12 at 34,373,907 bp
  • C to A, chromosome 12 at 71,946,520 bp
  • G to A, chromosome 12 at 84,411,270 bp
  • T to A, chromosome 13 at 11,587,254 bp
  • A to T, chromosome 13 at 18,139,020 bp
  • A to C, chromosome 13 at 36,988,856 bp
  • T to C, chromosome 13 at 59,598,046 bp
  • T to A, chromosome 14 at 50,643,162 bp
  • C to A, chromosome 14 at 63,163,404 bp
  • A to T, chromosome 14 at 70,421,593 bp
  • T to A, chromosome 15 at 28,421,678 bp
  • T to A, chromosome 15 at 31,276,897 bp
  • C to T, chromosome 15 at 82,779,611 bp
  • T to A, chromosome 15 at 89,092,766 bp
  • G to A, chromosome 16 at 3,866,638 bp
  • T to A, chromosome 16 at 25,763,306 bp
  • A to G, chromosome 16 at 88,829,156 bp
  • A to T, chromosome 17 at 21,262,288 bp
  • G to A, chromosome 17 at 23,820,618 bp
  • G to T, chromosome 17 at 24,509,793 bp
  • A to G, chromosome 17 at 27,886,864 bp
  • A to G, chromosome 17 at 65,976,069 bp
  • T to C, chromosome 17 at 94,764,828 bp
  • A to G, chromosome 18 at 42,214,776 bp
  • A to T, chromosome 18 at 61,077,668 bp
  • G to A, chromosome 18 at 62,014,891 bp
  • A to G, chromosome 18 at 75,119,161 bp
  • A to G, chromosome 18 at 84,670,829 bp
  • C to A, chromosome 19 at 53,006,175 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5033 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042624-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.