Beutler Mutagenetix

Strain Name:
C57BL/6J-MtgxR5060Btlr/Mmmh
Stock Number:
042650-MU
Citation ID:
RRID:MMRRC_042650-MU
Other Names:
R5060 (G1), C57BL/6J-MtgxR5060Btlr
Major Collection:

Gene Information

Ank2
Name: ankyrin 2, brain
Synonyms: Ankyrin-2, Gm4392, Ank-2, ankyrin B, Ankyrin-B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 109676
HGNC: HGNC:493
Homologene: null
Acvr2a
Name: activin receptor IIA
Synonyms: Acvr2, ActRIIa, tActRII
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 11480
HGNC: HGNC:173
Homologene: 20391
Ick
Name: intestinal cell kinase
Synonyms: 2210420N10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 56542
Homologene: 69218
Rlf
Name: rearranged L-myc fusion sequence
Synonyms: MommeD8, 9230110M18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 109263
Homologene: 8243
Bnc2
Name: basonuclin 2
Synonyms: 8430420F16Rik, 5031434M05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 242509
Homologene: 18243
Mark3
Name: MAP/microtubule affinity regulating kinase 3
Synonyms: ETK-1, A430080F22Rik, C-TAK1, 1600015G02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 17169
VEGA: 12
HGNC: HGNC:6897
Homologene: 55653
Zbtb40
Name: zinc finger and BTB domain containing 40
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 230848
Homologene: 8912
Poc1a
Name: POC1 centriolar protein A
Synonyms: 2510040D07Rik, Wdr51a, cha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 70235
Homologene: 51460
Melk
Name: maternal embryonic leucine zipper kinase
Synonyms: MPK38
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 17279
Homologene: 32111
Trpm4
Name: transient receptor potential cation channel, subfamily M, member 4
Synonyms: 1110030C19Rik, LTRPC4, TRPM4B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 68667
Homologene: 23033
Clca4b
Name: chloride channel accessory 4B
Synonyms: AI747448
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 99709
HGNC: HGNC:2018
Homologene: 40808
Adam33
Name: a disintegrin and metallopeptidase domain 33
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 110751
Homologene: 11881
Adamts12
Name: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 239337
VEGA: 15
Homologene: 12808
Reck
Name: reversion-inducing-cysteine-rich protein with kazal motifs
Synonyms: St15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 53614
Homologene: 9622
Gmds
Name: GDP-mannose 4, 6-dehydratase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 218138
HGNC: HGNC:4369
Homologene: 75968
Col4a3bp
Name: collagen, type IV, alpha 3 (Goodpasture antigen) binding protein
Synonyms: GPBP, 2810404O15Rik, 9230101K08Rik, ceramide transport protein, Cert
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 68018
VEGA: 13
HGNC: HGNC:2205
Homologene: 4173
Rabgef1
Name: RAB guanine nucleotide exchange factor (GEF) 1
Synonyms: Ras negative regulator Rabex-5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 56715
Homologene: 8720
Ttyh1
Name: tweety family member 1
Synonyms: 6330408P11Rik, 4930459B04Rik, tty
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 57776
Homologene: 10779
Pax7
Name: paired box 7
Synonyms: Pax-7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 18509
HGNC: HGNC:8621
Homologene: 55665
Ttn
Name: titin
Synonyms: shru, connectin, mdm, 2310057K23Rik, D330041I19Rik, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, L56, 2310074I15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 22138
Homologene: 130650
Ankrd52
Name: ankyrin repeat domain 52
Synonyms: G431002C21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 237615
Homologene: 18227
Xpr1
Name: xenotropic and polytropic retrovirus receptor 1
Synonyms: Syg1, suppressor of yeast Ga deletion, Rmc-1, Rmc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 19775
Homologene: 134226
Vmn1r73
Name: vomeronasal 1 receptor 73
Synonyms: V1rg2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 171237
HGNC: null
Homologene: 103842
Grm3
Name: glutamate receptor, metabotropic 3
Synonyms: Gprc1c, 0710001G23Rik, mGlu3, mGluR3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 108069
HGNC: HGNC:4595
Homologene: 651
Ccdc39
Name: coiled-coil domain containing 39
Synonyms: 4921507O14Rik, D3Ertd789e, b2b1735Clo, b2b1304Clo, b2b2025.1Clo, prh
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 51938
Homologene: 12149
Rhobtb3
Name: Rho-related BTB domain containing 3
Synonyms: 4930503C18Rik, 1700040C17Rik, 2610033K01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 73296
Homologene: 8932
Plcl1
Name: phospholipase C-like 1
Synonyms: PRIP-1, PLC-L, C230017K02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 227120
HGNC: HGNC:9063
Homologene: 38155
Abca6
Name: ATP-binding cassette, sub-family A (ABC1), member 6
Synonyms: 6330565N06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 76184
HGNC: HGNC:36
Homologene: 71264
Unc5d
Name: unc-5 netrin receptor D
Synonyms: D930029E11Rik, Unc5h4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 210801
Homologene: 15450
Pou6f1
Name: POU domain, class 6, transcription factor 1
Synonyms: cns-1, Emb, 2310038G18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 19009
HGNC: HGNC:9224
Homologene: 7703
Heatr9
Name: HEAT repeat containing 9
Synonyms: Gm11435
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 629303
Homologene: 51895
Vwde
Name: von Willebrand factor D and EGF domains
Synonyms: LOC232585
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 232585
Homologene: 35456
Als2cl
Name: ALS2 C-terminal like
Synonyms: D930044G19Rik, mRn.49018
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 235633
Homologene: 17152
Kif26b
Name: kinesin family member 26B
Synonyms: D230039L06Rik, N-11 kinesin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 269152
Homologene: 18623
Cyp2c65
Name: cytochrome P450, family 2, subfamily c, polypeptide 65
Synonyms: 2210009K14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 72303
HGNC: HGNC:2623
Homologene: 133566
A530064D06Rik
Name: RIKEN cDNA A530064D06 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 328830
VEGA: 17
HGNC: null
Homologene: 136361
Adprhl1
Name: ADP-ribosylhydrolase like 1
Synonyms: Arh2, D330008N11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 234072
Homologene: 16311
Gpcpd1
Name: glycerophosphocholine phosphodiesterase 1
Synonyms: 2310004G06Rik, 2310032D16Rik, Prei4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 74182
Homologene: 10478
Hcn1
Name: hyperpolarization activated cyclic nucleotide gated potassium channel 1
Synonyms: C630013B14Rik, hyperpolarization-activated, cyclic nucleotide-gated K+ 1, Bcng1, HAC2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 15165
HGNC: HGNC:4845
Homologene: 32093
Ggt5
Name: gamma-glutamyltransferase 5
Synonyms: GGT-REL, Ggtla1, gamma-glutamyl leukotrienase, GGL
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 23887
HGNC: HGNC:4260
Homologene: 55802
Olfr95
Name: olfactory receptor 95
Synonyms: MOR263-6, GA_x6K02T2PSCP-1651760-1650822
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 258506
HGNC: HGNC:8165
Homologene: 71990
Actg1
Name: actin, gamma, cytoplasmic 1
Synonyms: E51, Actl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 11465
HGNC: HGNC:144
Homologene: 74402
Mrpl41
Name: mitochondrial ribosomal protein L41
Synonyms: MRP-L27, Rpml27
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 107733
Homologene: 13036
Tff1
Name: trefoil factor 1
Synonyms: Bcei, PS2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 21784
Homologene: 2426
Olfr803
Name: olfactory receptor 803
Synonyms: MOR111-3, GA_x6K02T2PULF-11370664-11369738
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 258547
Homologene: 105153
5330439B14Rik
Name: RIKEN cDNA 5330439B14 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 321015
HGNC: null
Homologene: null
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 55,696,512 bp
  • T to C, chromosome 1 at 155,328,684 bp
  • G to A, chromosome 1 at 178,530,630 bp
  • A to G, chromosome 2 at 24,974,283 bp
  • A to G, chromosome 2 at 48,890,299 bp
  • A to T, chromosome 2 at 76,813,397 bp
  • C to T, chromosome 2 at 131,063,497 bp
  • A to T, chromosome 2 at 132,544,435 bp
  • C to T, chromosome 3 at 33,813,155 bp
  • T to C, chromosome 3 at 126,945,921 bp
  • T to A, chromosome 3 at 144,911,506 bp
  • C to T, chromosome 4 at 43,912,119 bp
  • T to C, chromosome 4 at 44,350,959 bp
  • C to A, chromosome 4 at 84,531,635 bp
  • A to G, chromosome 4 at 121,146,866 bp
  • A to G, chromosome 4 at 137,001,293 bp
  • A to T, chromosome 4 at 139,779,617 bp
  • A to T, chromosome 4 at 139,829,595 bp
  • A to G, chromosome 5 at 9,570,167 bp
  • A to G, chromosome 5 at 130,212,003 bp
  • C to T, chromosome 6 at 13,208,324 bp
  • A to G, chromosome 6 at 142,626,110 bp
  • C to T, chromosome 7 at 4,133,393 bp
  • A to G, chromosome 7 at 11,756,756 bp
  • G to T, chromosome 7 at 45,321,834 bp
  • A to G, chromosome 8 at 13,248,621 bp
  • A to T, chromosome 8 at 28,719,695 bp
  • C to T, chromosome 9 at 78,153,696 bp
  • G to T, chromosome 9 at 106,349,813 bp
  • C to T, chromosome 9 at 110,884,137 bp
  • T to C, chromosome 10 at 75,604,774 bp
  • C to T, chromosome 10 at 93,039,151 bp
  • T to A, chromosome 10 at 128,389,841 bp
  • C to T, chromosome 10 at 129,691,830 bp
  • T to C, chromosome 11 at 83,512,432 bp
  • A to G, chromosome 11 at 110,219,604 bp
  • A to G, chromosome 11 at 120,347,013 bp
  • T to C, chromosome 12 at 111,618,326 bp
  • A to G, chromosome 13 at 31,940,499 bp
  • A to C, chromosome 13 at 75,913,270 bp
  • A to T, chromosome 13 at 96,603,155 bp
  • A to T, chromosome 13 at 117,873,905 bp
  • T to C, chromosome 15 at 11,299,968 bp
  • A to T, chromosome 15 at 100,591,854 bp
  • A to G, chromosome 17 at 31,162,708 bp
  • C to A, chromosome 17 at 37,211,416 bp
  • A to T, chromosome 17 at 48,166,939 bp
  • T to C, chromosome 19 at 39,061,070 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5060 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042650-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.