Strain Name:
C57BL/6J-MtgxR5064Btlr/Mmmh
Stock Number:
042654-MU
Citation ID:
RRID:MMRRC_042654-MU
Other Names:
R5064 (G1), C57BL/6J-MtgxR5064Btlr
Major Collection:

Strain Information

Abca12
Name: ATP-binding cassette, sub-family A member 12
Synonyms: 4833417A11Rik, 4832428G11Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 74591
Homologene: 45441
Nup155
Name: nucleoporin 155
Synonyms: D930027M19Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 170762
VEGA: 15
HGNC: HGNC:8063
Homologene: 43155
Por
Name: cytochrome p450 oxidoreductase
Synonyms: 4933424M13Rik, CYPOR, NADH cytochrome P450 oxydoreductase, CPR
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 18984
HGNC: HGNC:9208
Homologene: 725
Tnrc6a
Name: trinucleotide repeat containing 6a
Synonyms: CAGH26, Tnrc6, D130023A07Rik, 3110054G10Rik, 2010321I05Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233833
Homologene: 41399
Mllt6
Name: myeloid/lymphoid or mixed-lineage leukemia; translocated to, 6
Synonyms: Af17
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 246198
HGNC: HGNC:7138
Homologene: 31347
Nasp
Name: nuclear autoantigenic sperm protein (histone-binding)
Synonyms: D4Ertd767e, Epcs32, 5033430J04Rik, Nasp-T
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 50927
HGNC: HGNC:7644
Homologene: 133894
Sfxn1
Name: sideroflexin 1
Synonyms: A930015P12Rik, 2810002O05Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 14057
VEGA: 13
Homologene: 41494
Pikfyve
Name: phosphoinositide kinase, FYVE type zinc finger containing
Synonyms: PipkIII, Pip5k3, 5230400C17Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 18711
Homologene: 32115
Nsd2
Name: nuclear receptor binding SET domain protein 2
Synonyms: 9430010A17Rik, 5830445G22Rik, D930023B08Rik, Whsc1l, C130020C13Rik, D030027O06Rik, Whsc1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 107823
Homologene: 26175
Plppr1
Name: phospholipid phosphatase related 1
Synonyms: E130309F12Rik, Lppr1, PRG-3
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 272031
Homologene: 9815
Pxylp1
Name: 2-phosphoxylose phosphatase 1
Synonyms: C130099A20Rik, 9430094M07Rik, Acpl2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235534
Homologene: 15931
Plaa
Name: phospholipase A2, activating protein
Synonyms: D4Ertd618e, Ufd3
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 18786
HGNC: HGNC:9043
Homologene: 3138
Ints2
Name: integrator complex subunit 2
Synonyms: 2810417D08Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 70422
Homologene: 10801
Zfp236
Name: zinc finger protein 236
Synonyms: LOC240456
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 329002
Homologene: 7198
Asxl3
Name: ASXL transcriptional regulator 3
Synonyms: D930044O18Rik, D430002O22Rik, C230079D11Rik, LOC381127
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 211961
Homologene: 19371
Mfng
Name: MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
Synonyms: manic fringe
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 17305
HGNC: HGNC:7038
Homologene: 1803
Grin1
Name: glutamate receptor, ionotropic, NMDA1 (zeta 1)
Synonyms: NR1, GluRzeta1, M100174, Rgsc174, Nmdar, NMDAR1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14810
HGNC: HGNC:4584
Homologene: 7187
Nr1h2
Name: nuclear receptor subfamily 1, group H, member 2
Synonyms: LXRbeta, RIP15, LXRB, Unr2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 22260
HGNC: HGNC:7965
Homologene: 21397
Ttn
Name: titin
Synonyms: connectin, shru, D330041I19Rik, 2310057K23Rik, mdm, D830007G01Rik, 2310074I15Rik, 1100001C23Rik, 2310036G12Rik, L56
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 22138
Homologene: 130650
Efhc1
Name: EF-hand domain (C-terminal) containing 1
Synonyms: myoclonin1, mRib72-1, 1700029F22Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 71877
Homologene: 10003
Wdr87-ps
Name: WD repeat domain 87, pseudogene
Synonyms: 4932431P20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 114675
Adamts7
Name: ADAM metallopeptidase with thrombospondin type 1 motif 7
Synonyms: ADAM-TS7
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 108153
HGNC: HGNC:223
Homologene: 22803
Thsd7a
Name: thrombospondin, type I, domain containing 7A
Synonyms: LOC330267
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 330267
Homologene: 46582
Gli3
Name: GLI-Kruppel family member GLI3
Synonyms: Bph, brachyphalangy
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 14634
HGNC: HGNC:4319
Homologene: 139
Mypn
Name: myopalladin
Synonyms: 1110056A04Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 68802
VEGA: 10
Homologene: 23778
Eml6
Name: echinoderm microtubule associated protein like 6
Synonyms: C230094A16Rik, 2900083P10Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 237711
Homologene: 104282
Vmn2r58
Name: vomeronasal 2, receptor 58
Synonyms: EG628422
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 628422
Cttnbp2
Name: cortactin binding protein 2
Synonyms: Cortbp2, 3010022N24Rik, 4732477G22Rik, ORF4, 9130022E09Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 30785
Homologene: 14125
Msh5
Name: mutS homolog 5
Synonyms: G7, Mut5
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 17687
HGNC: HGNC:7328
Homologene: 8415
Niban1
Name: niban apoptosis regulator 1
Synonyms: Niban, Fam129a
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 63913
Homologene: 62170
Ascl2
Name: achaete-scute family bHLH transcription factor 2
Synonyms: bHLHa45, 2410083I15Rik, Mash2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 17173
HGNC: HGNC:739
Homologene: 3789
Zfp952
Name: zinc finger protein 952
Synonyms: C920016K16Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 240067
Homologene: 128790
Serpina3a
Name: serine (or cysteine) peptidase inhibitor, clade A, member 3A
Synonyms: 4933406L18Rik, antitrypsin, alpha-1 antiproteinase,
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 74069
HGNC: HGNC:16
Nat8l
Name: N-acetyltransferase 8-like
Synonyms: Shati, 1110038O08Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 269642
Homologene: 19546
Vmn1r55
Name: vomeronasal 1 receptor 55
Synonyms: LOC236535, LOC384522, V1rd5
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 384522
Homologene: 41799
Vta1
Name: vesicle (multivesicular body) trafficking 1
Synonyms: 1110001D18Rik, 1110059P08Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 66201
Homologene: 6473
Gm26672
Name: predicted gene, 26672
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 20,974,963 bp
  • A to G, chromosome 1 at 65,253,407 bp
  • A to G, chromosome 1 at 71,300,960 bp
  • A to G, chromosome 1 at 151,689,659 bp
  • G to T, chromosome 2 at 25,303,831 bp
  • T to A, chromosome 2 at 76,889,407 bp
  • A to T, chromosome 4 at 49,319,974 bp
  • G to A, chromosome 4 at 94,574,043 bp
  • A to G, chromosome 4 at 116,611,970 bp
  • T to A, chromosome 5 at 33,885,630 bp
  • T to C, chromosome 5 at 33,996,869 bp
  • T to C, chromosome 5 at 135,733,795 bp
  • G to T, chromosome 6 at 12,330,952 bp
  • T to C, chromosome 6 at 18,448,279 bp
  • A to T, chromosome 7 at 5,146,929 bp
  • A to G, chromosome 7 at 29,535,655 bp
  • A to T, chromosome 7 at 41,837,110 bp
  • G to A, chromosome 7 at 44,551,649 bp
  • T to C, chromosome 7 at 123,186,723 bp
  • T to C, chromosome 7 at 142,968,259 bp
  • T to A, chromosome 9 at 90,195,830 bp
  • T to G, chromosome 9 at 96,854,800 bp
  • G to T, chromosome 10 at 14,705,478 bp
  • T to A, chromosome 10 at 63,123,371 bp
  • C to T, chromosome 11 at 29,749,300 bp
  • C to T, chromosome 11 at 86,249,274 bp
  • C to A, chromosome 11 at 97,673,949 bp
  • G to A, chromosome 12 at 104,116,189 bp
  • T to C, chromosome 13 at 15,613,870 bp
  • T to C, chromosome 13 at 54,085,569 bp
  • C to A, chromosome 15 at 8,135,870 bp
  • C to T, chromosome 15 at 78,764,388 bp
  • T to C, chromosome 17 at 33,002,959 bp
  • G to T, chromosome 17 at 35,043,783 bp
  • G to T, chromosome 18 at 22,516,019 bp
  • C to T, chromosome 18 at 37,755,970 bp
  • A to G, chromosome 18 at 82,691,576 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5064 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042654-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.