Strain Name:
C57BL/6J-MtgxR5065Btlr/Mmmh
Stock Number:
042655-MU
Citation ID:
RRID:MMRRC_042655-MU
Other Names:
R5065 (G1), C57BL/6J-MtgxR5065Btlr
Major Collection:

Gene Information

Sema3c
Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C
Synonyms: Semae, 1110036B02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 20348
Homologene: 36201
Snd1
Name: staphylococcal nuclease and tudor domain containing 1
Synonyms: p100 co-activator, Tudor-SN
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 56463
Homologene: 8665
Ptpn23
Name: protein tyrosine phosphatase, non-receptor type 23
Synonyms: PTP-TD14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 104831
Homologene: 135706
Kmt2a
Name: lysine (K)-specific methyltransferase 2A
Synonyms: trithorax Drosophila, HTRX1, ALL-1, All1, Cxxc7, Mll, Mll1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 214162
HGNC: HGNC:7132
Homologene: 4338
Polr3b
Name: polymerase (RNA) III (DNA directed) polypeptide B
Synonyms: RPC2, 2700078H01Rik, A330032P03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 70428
VEGA: 10
Homologene: 6981
Sdha
Name: succinate dehydrogenase complex, subunit A, flavoprotein (Fp)
Synonyms: SDHF, SDH2, FP, 2310034D06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 66945
Homologene: 3073
Wap
Name: whey acidic protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 22373
Homologene: 137242
Hhipl2
Name: hedgehog interacting protein-like 2
Synonyms: 4930507C10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 78772
Homologene: 11690
Slc39a7
Name: solute carrier family 39 (zinc transporter), member 7
Synonyms: Ke-4, H-2Ke4, KE4, Ring5, H2-Ke4, Zip7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 14977
HGNC: HGNC:4927
Homologene: 5072
Tdrp
Name: testis development related protein
Synonyms: 2610019F03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 72148
Homologene: 18348
Dennd2a
Name: DENN/MADD domain containing 2A
Synonyms: B930096L08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 209773
Homologene: 35238
Asxl3
Name: ASXL transcriptional regulator 3
Synonyms: LOC381127, D930044O18Rik, D430002O22Rik, C230079D11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 211961
Homologene: 19371
Rasgef1b
Name: RasGEF domain family, member 1B
Synonyms: Gpig4, 4732452O09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 320292
Homologene: 14860
Cspg4b
Name: chondroitin sulfate proteoglycan 4B
Synonyms: BC067074
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 408066
Map3k5
Name: mitogen-activated protein kinase kinase kinase 5
Synonyms: ASK1, Mekk5, 7420452D20Rik, ASK
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 26408
HGNC: HGNC:6857
Homologene: 38114
Pkhd1l1
Name: polycystic kidney and hepatic disease 1-like 1
Synonyms: D86 mRNA, PKHDL1, fibrocystin L
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 192190
Homologene: 16332
Gm4847
Name: predicted gene 4847
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226604
Homologene: 101040
Sntg1
Name: syntrophin, gamma 1
Synonyms: G1SYN, SYN4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 71096
Homologene: 56834
Mroh4
Name: maestro heat-like repeat family member 4
Synonyms: 1700016M24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 69439
Homologene: 72545
Dock3
Name: dedicator of cyto-kinesis 3
Synonyms: PBP, Moca
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 208869
HGNC: HGNC:2989
Homologene: 21030
Stxbp5
Name: syntaxin binding protein 5 (tomosyn)
Synonyms: 0710001E20Rik, LGL3, 4930565N16Rik, tomosyn 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 78808
Homologene: 16402
Aldh9a1
Name: aldehyde dehydrogenase 9, subfamily A1
Synonyms: TMABA-DH, ESTM40
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 56752
HGNC: HGNC:412
Homologene: 55483
Vmn1r58
Name: vomeronasal 1 receptor 58
Synonyms: V3R4, V1rd4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 81014
Homologene: 41799
Map6
Name: microtubule-associated protein 6
Synonyms: F-STOP, STOP, 2810411E12Rik, Mtap6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 17760
HGNC: HGNC:6868
Homologene: 7850
Pcsk6
Name: proprotein convertase subtilisin/kexin type 6
Synonyms: PACE4, SPC4, b2b2830Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 18553
HGNC: HGNC:8569
Homologene: 20569
Ighg2c
Name: immunoglobulin heavy constant gamma 2C
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 404711
Sipa1l2
Name: signal-induced proliferation-associated 1 like 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 244668
Homologene: 18956
Tacr1
Name: tachykinin receptor 1
Synonyms: neurokinin receptor 1, NK-1R, NK1-R, NK1 receptor, Tac1r, substance p receptor, SPr
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 21336
Homologene: 20288
Or10g6
Name: olfactory receptor family 10 subfamily G member 6
Synonyms: GA_x6K02T2PVTD-33720892-33721824, MOR223-8, Olfr981
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 258283
VEGA: 9
Homologene: 64849
Hsfy2
Name: heat shock transcription factor, Y-linked 2
Synonyms: 4933413G11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 71066
Homologene: 134090
2310009B15Rik
Name: RIKEN cDNA 2310009B15 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 69549
Homologene: 19923
Gm8674
Name: predicted gene 8674
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 667507
VEGA: 13
Homologene: 140547
Bcl2l10
Name: Bcl2-like 10
Synonyms: Boo, Diva
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 12049
VEGA: 9
HGNC: HGNC:993
Homologene: 8396
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 8,363,439 bp
  • G to A, chromosome 1 at 56,636,467 bp
  • T to A, chromosome 1 at 138,852,155 bp
  • A to T, chromosome 1 at 166,634,790 bp
  • A to G, chromosome 1 at 167,352,559 bp
  • A to T, chromosome 1 at 183,426,672 bp
  • A to G, chromosome 5 at 17,727,617 bp
  • G to A, chromosome 5 at 99,234,573 bp
  • C to A, chromosome 6 at 28,888,240 bp
  • C to A, chromosome 6 at 39,495,176 bp
  • G to A, chromosome 6 at 82,554,878 bp
  • A to G, chromosome 7 at 5,410,835 bp
  • A to G, chromosome 7 at 65,910,299 bp
  • A to G, chromosome 7 at 99,336,710 bp
  • T to C, chromosome 8 at 13,953,791 bp
  • T to C, chromosome 8 at 125,491,585 bp
  • A to G, chromosome 9 at 40,023,250 bp
  • T to C, chromosome 9 at 44,842,700 bp
  • A to T, chromosome 9 at 75,347,979 bp
  • A to G, chromosome 9 at 106,955,684 bp
  • A to T, chromosome 9 at 110,398,188 bp
  • A to T, chromosome 10 at 9,770,551 bp
  • A to G, chromosome 10 at 20,082,467 bp
  • C to A, chromosome 10 at 84,632,538 bp
  • A to T, chromosome 11 at 6,636,840 bp
  • T to C, chromosome 12 at 113,288,088 bp
  • T to C, chromosome 13 at 49,902,577 bp
  • G to A, chromosome 13 at 74,344,896 bp
  • T to A, chromosome 13 at 113,320,919 bp
  • T to A, chromosome 15 at 44,582,293 bp
  • C to T, chromosome 15 at 74,628,270 bp
  • A to G, chromosome 17 at 34,031,059 bp
  • C to G, chromosome 18 at 22,525,299 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5065 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042655-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.