Strain Name:
C57BL/6J-MtgxR5066Btlr/Mmmh
Stock Number:
042656-MU
Citation ID:
RRID:MMRRC_042656-MU
Other Names:
R5066 (G1), C57BL/6J-MtgxR5066Btlr
Major Collection:

Gene Information

Atrn
Name: attractin
Synonyms: Mgca
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 11990
HGNC: HGNC:885
Homologene: 22542
Nedd4
Name: neural precursor cell expressed, developmentally down-regulated 4
Synonyms: Nedd4-1, Nedd4, Nedd4a, E430025J12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 17999
HGNC: HGNC:7727
Homologene: 134533
Atp1a1
Name: ATPase, Na+/K+ transporting, alpha 1 polypeptide
Synonyms: Atpa-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 11928
HGNC: HGNC:799
Homologene: 564
Ndufv1
Name: NADH:ubiquinone oxidoreductase core subunit V1
Synonyms: 24 kDa (FP)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 17995
HGNC: HGNC:7716
Homologene: 5151
Ppil2
Name: peptidylprolyl isomerase (cyclophilin)-like 2
Synonyms: C130078A06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 66053
HGNC: HGNC:9261
Homologene: 8643
Snd1
Name: staphylococcal nuclease and tudor domain containing 1
Synonyms: p100 co-activator, Tudor-SN
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 56463
Homologene: 8665
Bcl2l13
Name: BCL2-like 13 (apoptosis facilitator)
Synonyms: BCL-RAMBO, Mil1, E430016C20Rik, Mil-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 94044
Homologene: 9111
Tle1
Name: transducin-like enhancer of split 1
Synonyms: Grg1, Estm14, C230057C06Rik, Tle4l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 21885
Homologene: 21058
Nfx1
Name: nuclear transcription factor, X-box binding 1
Synonyms: TEG-42, 3000003M19Rik, 1300017N15Rik, Tex42
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 74164
HGNC: HGNC:7803
Homologene: 1875
Chic2
Name: cysteine-rich hydrophobic domain 2
Synonyms: BTL, 1700081B18Rik, 4930502K01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 74277
HGNC: HGNC:1935
Homologene: 8105
Ice2
Name: interactor of little elongation complex ELL subunit 2
Synonyms: B230343B06Rik, Narg2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 93697
Homologene: 11618
Tnc
Name: tenascin C
Synonyms: Hxb, TN-C, TN, C130033P17Rik, tenascin-C, cytotactin, hexabrachion
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 21923
HGNC: HGNC:5318
Homologene: 55636
Apc
Name: APC, WNT signaling pathway regulator
Synonyms: Min, CC1, adenomatosis polyposis coli
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 11789
HGNC: HGNC:583
Homologene: 30950
Drg1
Name: developmentally regulated GTP binding protein 1
Synonyms: Nedd3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 13494
HGNC: HGNC:3029
Homologene: 3060
Mpp7
Name: membrane protein, palmitoylated 7 (MAGUK p55 subfamily member 7)
Synonyms: 5430426E14Rik, 2810038M04Rik, LOC381166, 1110068J02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 75739
VEGA: 18
Homologene: 1455
Asxl3
Name: ASXL transcriptional regulator 3
Synonyms: LOC381127, D930044O18Rik, D430002O22Rik, C230079D11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 211961
Homologene: 19371
Agtpbp1
Name: ATP/GTP binding protein 1
Synonyms: Nna1, 2900054O13Rik, 4930445M19Rik, 1700020N17Rik, 5730402G09Rik, 2310001G17Rik, Ccp1, atms
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 67269
Homologene: 9067
Syk
Name: spleen tyrosine kinase
Synonyms: Sykb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 20963
Homologene: 2390
Aldh1a2
Name: aldehyde dehydrogenase family 1, subfamily A2
Synonyms: Aldh1a7, Raldh2, retinaldehyde dehydrogenase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 19378
Homologene: 68368
Slc45a2
Name: solute carrier family 45, member 2
Synonyms: Dbr, bls, Aim-1, Aim1, dominant brown, blanc-sale, Matp, Oca4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 22293
Homologene: 9412
Syne2
Name: spectrin repeat containing, nuclear envelope 2
Synonyms: nesprin-2, syne-2, D12Ertd777e, 6820443O06Rik, Nesp2g, Cpfl8, diminished cone electroretinogram, dice
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 319565
Homologene: 56700
Sybu
Name: syntabulin (syntaxin-interacting)
Synonyms: A830027B17Rik, Golsyn/Syntabulin, 5730410E15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 319613
VEGA: 15
Homologene: 9838
Gm11639
Name: predicted gene 11639
Synonyms: Efcab15, Gm11639
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 105242472
Parp10
Name: poly (ADP-ribose) polymerase family, member 10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 671535
Homologene: 53133
Mndal
Name: myeloid nuclear differentiation antigen like
Synonyms: Ifi212
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 100040462
HGNC: HGNC:5395
Homologene: 115929
Heg1
Name: heart development protein with EGF-like domains 1
Synonyms: LOC268884, 5530401I02Rik, 9530025L16Rik, 4632417D23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 77446
Homologene: 35276
Abca5
Name: ATP-binding cassette, sub-family A (ABC1), member 5
Synonyms: ABC13, B930033A02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217265
HGNC: HGNC:35
Homologene: 10263
Hadhb
Name: hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit beta
Synonyms: 4930479F15Rik, Mtpb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231086
HGNC: HGNC:4803
Homologene: 153
Slco1b2
Name: solute carrier organic anion transporter family, member 1b2
Synonyms: mlst-1, Slc21a6, Slc21a10, Oatp1b2, 7330442B20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 28253
Homologene: 75119
Slc2a7
Name: solute carrier family 2 (facilitated glucose transporter), member 7
Synonyms: OTTMUSG00000010396
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 435818
Homologene: 72470
Thsd4
Name: thrombospondin, type I, domain containing 4
Synonyms: B230114P05Rik, ADAMTSL6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 207596
Homologene: 49796
4930544D05Rik
Name: RIKEN cDNA 4930544D05 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 668433
Homologene: 109413
Spata13
Name: spermatogenesis associated 13
Synonyms: ESTM11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 219140
Homologene: 19482
Flt4
Name: FMS-like tyrosine kinase 4
Synonyms: VEGFR3, VEGFR-3, Flt-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 14257
HGNC: HGNC:3767
Homologene: 7321
Mical1
Name: microtubule associated monooxygenase, calponin and LIM domain containing 1
Synonyms: Nical
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 171580
Homologene: 11246
Sult1c1
Name: sulfotransferase family, cytosolic, 1C, member 1
Synonyms: (PST)G, mOLFST, P-SULT, Stp2, Sult1a2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 20888
Homologene: 56817
Padi1
Name: peptidyl arginine deiminase, type I
Synonyms: Pdi1, Pad type 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 18599
Homologene: 7881
Atp13a2
Name: ATPase type 13A2
Synonyms: 1110012E06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 74772
Homologene: 56940
Setbp1
Name: SET binding protein 1
Synonyms: Seb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 240427
Homologene: 9192
Tmprss11a
Name: transmembrane protease, serine 11a
Synonyms: LOC194597
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 194597
Homologene: 62723
Olfr9
Name: olfactory receptor 9
Synonyms: MOR269-3, GA_x6K02T2PULF-10675610-10676548
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 18373
Homologene: 85121
Olfr773
Name: olfactory receptor 773
Synonyms: GA_x6K02T2PULF-10872859-10871923, MOR114-15, Olfr773-ps1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 257664
Homologene: 123776
Tmbim4
Name: transmembrane BAX inhibitor motif containing 4
Synonyms: 0610007H07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 68212
Homologene: 56740
BC049762
Name: cDNA sequence BC049762
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 193286
Homologene: 77615
Igkv8-21
Name: immunoglobulin kappa variable 8-21
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 620400
AC154247.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 173,875,663 bp
  • T to C, chromosome 2 at 130,994,193 bp
  • C to T, chromosome 3 at 101,582,104 bp
  • A to G, chromosome 4 at 40,991,868 bp
  • C to A, chromosome 4 at 63,975,229 bp
  • A to G, chromosome 4 at 72,158,267 bp
  • T to C, chromosome 4 at 140,829,437 bp
  • G to A, chromosome 4 at 141,005,138 bp
  • T to A, chromosome 4 at 150,160,116 bp
  • T to C, chromosome 5 at 30,164,096 bp
  • A to G, chromosome 5 at 75,027,156 bp
  • A to G, chromosome 5 at 86,420,000 bp
  • C to A, chromosome 6 at 28,888,240 bp
  • G to A, chromosome 6 at 70,315,443 bp
  • T to A, chromosome 6 at 120,887,021 bp
  • G to A, chromosome 6 at 141,652,552 bp
  • A to G, chromosome 9 at 59,976,332 bp
  • A to C, chromosome 9 at 69,408,291 bp
  • G to A, chromosome 9 at 71,281,700 bp
  • T to A, chromosome 9 at 72,710,519 bp
  • G to A, chromosome 10 at 41,478,484 bp
  • C to T, chromosome 10 at 120,217,632 bp
  • G to A, chromosome 10 at 128,990,791 bp
  • T to C, chromosome 10 at 129,186,564 bp
  • A to T, chromosome 11 at 3,259,353 bp
  • A to G, chromosome 11 at 49,634,163 bp
  • A to G, chromosome 11 at 51,254,424 bp
  • A to T, chromosome 11 at 70,616,591 bp
  • A to G, chromosome 11 at 104,720,664 bp
  • A to T, chromosome 11 at 110,309,350 bp
  • A to G, chromosome 12 at 75,966,551 bp
  • T to A, chromosome 13 at 52,641,982 bp
  • T to A, chromosome 13 at 59,474,550 bp
  • T to C, chromosome 14 at 60,750,089 bp
  • C to A, chromosome 15 at 11,012,607 bp
  • A to G, chromosome 15 at 44,677,644 bp
  • A to G, chromosome 15 at 76,240,946 bp
  • A to G, chromosome 16 at 17,109,675 bp
  • G to T, chromosome 16 at 33,738,671 bp
  • T to C, chromosome 17 at 49,096,420 bp
  • T to A, chromosome 17 at 53,973,998 bp
  • T to C, chromosome 18 at 7,513,002 bp
  • C to G, chromosome 18 at 22,525,299 bp
  • T to A, chromosome 18 at 34,316,105 bp
  • A to T, chromosome 18 at 78,857,299 bp
  • C to T, chromosome 19 at 4,011,366 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5066 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042656-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.