Strain Name:
C57BL/6J-MtgxR5094Btlr/Mmmh
Stock Number:
042683-MU
Citation ID:
RRID:MMRRC_042683-MU
Other Names:
R5094 (G1), C57BL/6J-MtgxR5094Btlr
Major Collection:

Gene Information

Chrnb4
Name: cholinergic receptor, nicotinic, beta polypeptide 4
Synonyms: Acrb-4, Acrb4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 108015
VEGA: 9
HGNC: HGNC:1964
Homologene: 20196
Chat
Name: choline acetyltransferase
Synonyms: B230380D24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 12647
VEGA: 14
HGNC: HGNC:1912
Homologene: 40693
Ppp1r13b
Name: protein phosphatase 1, regulatory subunit 13B
Synonyms: ASPP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 21981
VEGA: 12
Homologene: 9090
Fgfr1
Name: fibroblast growth factor receptor 1
Synonyms: Hspy, Fgfr-1, Flt-2, FGFR-I, Fr1, Eask
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 14182
HGNC: HGNC:3688
Homologene: 69065
Dnajc2
Name: DnaJ heat shock protein family (Hsp40) member C2
Synonyms: Zrf2, MIDA1, Mida1, Zrf1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 22791
Homologene: 31656
Cep290
Name: centrosomal protein 290
Synonyms: Nphp6, Kiaa, b2b1752Clo, b2b1454Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 216274
VEGA: 10
Homologene: 77213
Hunk
Name: hormonally upregulated Neu-associated kinase
Synonyms: Bstk1, Mak-v
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 26559
VEGA: 16
Homologene: 8742
Phip
Name: pleckstrin homology domain interacting protein
Synonyms: Wdr11, 2810004D21Rik, Ndrp, 4632404O06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 83946
Homologene: 41209
Smtnl2
Name: smoothelin-like 2
Synonyms: D130058I21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 276829
Homologene: 82367
Tmem135
Name: transmembrane protein 135
Synonyms: 2810439K08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 72759
Homologene: 11295
Pah
Name: phenylalanine hydroxylase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 18478
HGNC: HGNC:8582
Homologene: 234
C3
Name: complement component 3
Synonyms: complement factor 3, Plp, acylation stimulating protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 12266
HGNC: HGNC:1318
Homologene: 68031
Bicra
Name: BRD4 interacting chromatin remodeling complex associated protein
Synonyms: Gltscr1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 243842
HGNC: HGNC:4332
Homologene: 9250
Tnrc6c
Name: trinucleotide repeat containing 6C
Synonyms: 9930033H14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217351
Homologene: 23137
Pfdn2
Name: prefoldin 2
Synonyms: W48336, ESTM27
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 18637
HGNC: HGNC:8867
Homologene: 7887
Pigg
Name: phosphatidylinositol glycan anchor biosynthesis, class G
Synonyms: Gpi7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 433931
Homologene: 39421
Hivep2
Name: human immunodeficiency virus type I enhancer binding protein 2
Synonyms: MIBP1, Schnurri-2, Gm20114, Shn-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 15273
HGNC: HGNC:4921
Homologene: 4900
Eml1
Name: echinoderm microtubule associated protein like 1
Synonyms: A930030P13Rik, ELP79, 1110008N23Rik, heco
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 68519
HGNC: HGNC:3330
Homologene: 20931
Plcb2
Name: phospholipase C, beta 2
Synonyms: B230205M18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 18796
HGNC: HGNC:9055
Homologene: 20957
Sema3d
Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D
Synonyms: 4631426B19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 108151
Homologene: 23664
4930407I10Rik
Name: RIKEN cDNA 4930407I10 gene
Synonyms: LOC328573
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 328573
VEGA: 15
Homologene: 130065
Gucy1a2
Name: guanylate cyclase 1, soluble, alpha 2
Synonyms: 6330407I18Rik, A230060L24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 234889
HGNC: HGNC:4684
Homologene: 47953
Slc5a1
Name: solute carrier family 5 (sodium/glucose cotransporter), member 1
Synonyms: sodium glucose cotransporter 1, Sglt1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 20537
Homologene: 55456
Gimap3
Name: GTPase, IMAP family member 3
Synonyms: 2010110D23Rik, Ian4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 83408
Homologene: 41257
Gm10472
Name: predicted gene 10472
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Gm12185
Name: predicted gene 12185
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 620913
Homologene: 83188
Pex13
Name: peroxisomal biogenesis factor 13
Synonyms: 2610008O20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 72129
HGNC: HGNC:8855
Homologene: 1967
Olfr1135
Name: olfactory receptor 1135
Synonyms: MOR177-2, GA_x6K02T2Q125-49177531-49176599
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258654
Homologene: 17331
Dph7
Name: diphthamine biosynethesis 7
Synonyms: Wdr85, 2810443J12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 67228
Homologene: 5432
Slc22a6
Name: solute carrier family 22 (organic anion transporter), member 6
Synonyms: Oat1, NKT, Orctl1, mOat1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 18399
VEGA: 19
Homologene: 16813
Olfr1051
Name: olfactory receptor 1051
Synonyms: GA_x6K02T2Q125-47756192-47755266, MOR187-5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 404324
Homologene: 77387
Atp10d
Name: ATPase, class V, type 10D
Synonyms: IMAGE:1069176, D5Buc24e, 9830145H18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231287
Ifit3b
Name: interferon-induced protein with tetratricopeptide repeats 3B
Synonyms: I830012O16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 667370
HGNC: HGNC:5411
Homologene: 1189
Spata31d1a
Name: spermatogenesis associated 31 subfamily D, member 1A
Synonyms: Fam75d1a, Fam75d3, 1700013B16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 72219
VEGA: 13
Homologene: 122131
Hnrnpa1l2-ps2
Name: heterogeneous nuclear ribonucleoprotein A1-like 2, pseudogene 2
Synonyms: Gm5803
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 545091
Homologene: 134075
Tlcd2
Name: TLC domain containing 2
Synonyms: 2010305C02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 380712
Homologene: 57070
Igkv13-54-1
Name: immunoglobulin kappa chain variable 13-54-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 692105
Mucl1
Name: mucin-like 1
Synonyms: Spt2, Spt-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 20771
Homologene: 137219
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 171,356,499 bp
  • C to A, chromosome 2 at 24,971,659 bp
  • T to A, chromosome 2 at 86,276,040 bp
  • C to T, chromosome 2 at 87,671,830 bp
  • T to C, chromosome 2 at 118,723,134 bp
  • C to T, chromosome 5 at 12,463,241 bp
  • T to C, chromosome 5 at 21,776,732 bp
  • T to C, chromosome 5 at 24,451,321 bp
  • A to G, chromosome 5 at 33,158,280 bp
  • T to C, chromosome 5 at 72,297,018 bp
  • A to G, chromosome 5 at 108,336,257 bp
  • T to C, chromosome 6 at 48,765,372 bp
  • G to T, chromosome 6 at 69,617,266 bp
  • A to G, chromosome 7 at 15,975,371 bp
  • A to G, chromosome 7 at 89,143,793 bp
  • C to T, chromosome 8 at 25,570,165 bp
  • T to A, chromosome 9 at 3,865,443 bp
  • T to C, chromosome 9 at 55,035,313 bp
  • C to T, chromosome 9 at 82,871,844 bp
  • T to C, chromosome 10 at 14,132,149 bp
  • T to A, chromosome 10 at 87,538,219 bp
  • T to C, chromosome 10 at 92,898,999 bp
  • A to G, chromosome 10 at 100,567,030 bp
  • A to G, chromosome 11 at 23,655,441 bp
  • T to A, chromosome 11 at 48,907,548 bp
  • T to A, chromosome 11 at 72,400,385 bp
  • T to C, chromosome 11 at 75,469,814 bp
  • T to C, chromosome 11 at 117,721,046 bp
  • T to C, chromosome 12 at 108,536,311 bp
  • A to G, chromosome 12 at 111,843,610 bp
  • A to G, chromosome 13 at 59,705,044 bp
  • T to A, chromosome 14 at 32,408,939 bp
  • G to A, chromosome 15 at 22,714,539 bp
  • G to T, chromosome 15 at 82,062,682 bp
  • A to G, chromosome 15 at 103,755,403 bp
  • A to G, chromosome 16 at 90,496,666 bp
  • A to T, chromosome 17 at 57,225,033 bp
  • T to C, chromosome 19 at 8,626,177 bp
  • T to A, chromosome 19 at 34,612,548 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5094 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042683-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.