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Strain Name:
C57BL/6J-MtgxR5126Btlr/Mmmh
Stock Number:
042714-MU
Citation ID:
RRID:MMRRC_042714-MU
Other Names:
R5126 (G1), C57BL/6J-MtgxR5126Btlr
Major Collection:

Strain Information

Tln2
Name: talin 2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 70549
VEGA: 9
Homologene: 56692
Kcna6
Name: potassium voltage-gated channel, shaker-related, subfamily, member 6
Synonyms: Kv1.6, MK1.6
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 16494
HGNC: HGNC:6225
Homologene: 1684
Ptprk
Name: protein tyrosine phosphatase receptor type K
Synonyms: PTPk, RPTPkappa
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 19272
VEGA: 10
HGNC: HGNC:9674
Homologene: 55693
Akap10
Name: A kinase anchor protein 10
Synonyms: D-AKAP2, 1500031L16Rik, B130049N18Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 56697
HGNC: HGNC:368
Homologene: 32452
Ahdc1
Name: AT hook, DNA binding motif, containing 1
Synonyms: D030015G18Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230793
Homologene: 17144
Mbd4
Name: methyl-CpG binding domain protein 4
Synonyms: Med1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 17193
HGNC: HGNC:6919
Homologene: 2916
Birc2
Name: baculoviral IAP repeat-containing 2
Synonyms: mcIAP1, MIAP1, IAP1, MIHB, Api1, cIAP1, cIAP-1, HIAP1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 11797
HGNC: HGNC:590
Homologene: 900
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 85,844,607 bp
  • A to G, chromosome 1 at 173,590,015 bp
  • G to T, chromosome 2 at 24,980,044 bp
  • G to A, chromosome 2 at 26,996,915 bp
  • C to T, chromosome 3 at 64,259,319 bp
  • T to A, chromosome 3 at 107,104,234 bp
  • T to A, chromosome 3 at 144,889,670 bp
  • A to C, chromosome 4 at 72,199,503 bp
  • C to T, chromosome 4 at 101,003,104 bp
  • T to A, chromosome 4 at 133,063,522 bp
  • T to C, chromosome 4 at 139,302,532 bp
  • C to A, chromosome 4 at 141,774,509 bp
  • T to C, chromosome 4 at 155,000,519 bp
  • G to T, chromosome 5 at 143,307,946 bp
  • A to G, chromosome 5 at 147,527,198 bp
  • T to A, chromosome 6 at 13,187,260 bp
  • T to A, chromosome 6 at 88,485,901 bp
  • T to A, chromosome 6 at 115,848,968 bp
  • T to A, chromosome 6 at 119,317,439 bp
  • T to C, chromosome 6 at 126,738,732 bp
  • A to T, chromosome 6 at 141,815,582 bp
  • T to C, chromosome 7 at 7,136,624 bp
  • T to A, chromosome 7 at 12,991,320 bp
  • A to C, chromosome 7 at 16,961,317 bp
  • A to G, chromosome 7 at 100,583,019 bp
  • A to G, chromosome 7 at 105,753,517 bp
  • A to T, chromosome 7 at 141,651,299 bp
  • G to T, chromosome 8 at 94,474,671 bp
  • T to A, chromosome 8 at 105,334,650 bp
  • T to A, chromosome 8 at 119,199,012 bp
  • T to C, chromosome 8 at 121,753,048 bp
  • C to T, chromosome 9 at 7,836,933 bp
  • A to G, chromosome 9 at 45,787,424 bp
  • A to G, chromosome 9 at 67,258,535 bp
  • A to T, chromosome 9 at 88,382,099 bp
  • G to A, chromosome 9 at 92,488,741 bp
  • A to T, chromosome 9 at 105,413,149 bp
  • A to T, chromosome 9 at 122,975,758 bp
  • G to T, chromosome 10 at 28,575,644 bp
  • T to A, chromosome 11 at 23,491,036 bp
  • T to C, chromosome 11 at 53,186,561 bp
  • T to A, chromosome 11 at 59,077,063 bp
  • C to T, chromosome 11 at 61,916,189 bp
  • A to T, chromosome 11 at 71,181,533 bp
  • A to G, chromosome 11 at 86,712,669 bp
  • C to A, chromosome 11 at 103,882,792 bp
  • T to A, chromosome 11 at 103,882,793 bp
  • C to A, chromosome 12 at 4,850,617 bp
  • A to G, chromosome 12 at 4,866,183 bp
  • G to A, chromosome 13 at 95,978,108 bp
  • C to G, chromosome 13 at 100,781,775 bp
  • T to C, chromosome 14 at 50,360,830 bp
  • A to G, chromosome 15 at 9,194,701 bp
  • A to G, chromosome 15 at 36,040,644 bp
  • A to C, chromosome 15 at 75,111,643 bp
  • C to T, chromosome 16 at 87,947,859 bp
  • T to C, chromosome 17 at 26,121,640 bp
  • G to T, chromosome 17 at 37,563,828 bp
  • T to C, chromosome 18 at 77,017,598 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5126 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042714-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.