Strain Name:
C57BL/6J-MtgxR5127Btlr/Mmmh
Stock Number:
042715-MU
Citation ID:
RRID:MMRRC_042715-MU
Other Names:
R5127 (G1), C57BL/6J-MtgxR5127Btlr
Major Collection:

Strain Information

Pknox1
Name: Pbx/knotted 1 homeobox
Synonyms: PREP1, D17Wsu76e
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18771
HGNC: HGNC:9022
Homologene: 3363
Dnajc11
Name: DnaJ heat shock protein family (Hsp40) member C11
Synonyms: E030019A03Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230935
Homologene: 14558
Ranbp1
Name: RAN binding protein 1
Synonyms: Htf9a
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 19385
HGNC: HGNC:9847
Homologene: 21600
Reps1
Name: RalBP1 associated Eps domain containing protein
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 19707
Homologene: 7515
Prrc2c
Name: proline-rich coiled-coil 2C
Synonyms: 9630039I18Rik, 1810043M20Rik, Bat2d, Bat2l2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226562
Homologene: 41015
Ufl1
Name: UFM1 specific ligase 1
Synonyms: Rcad, Maxer, 1810074P20Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 67490
Homologene: 9093
Kcnh5
Name: potassium voltage-gated channel, subfamily H (eag-related), member 5
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 238271
VEGA: 12
HGNC: HGNC:6254
Homologene: 15858
Zfp369
Name: zinc finger protein 369
Synonyms: NRIF2, D230020H11Rik, B930030B22Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 170936
Homologene: 129707
Ccnb1
Name: cyclin B1
Synonyms: Cycb-4, Ccnb1-rs13
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 268697
HGNC: HGNC:1579
Homologene: 68982
Dag1
Name: dystroglycan 1
Synonyms: dystrophin associated glycoprotein 1, DG, D9Wsu13e, alpha-dystroglycan, beta-dystroglycan
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 13138
HGNC: HGNC:2666
Homologene: 3234
Farsa
Name: phenylalanyl-tRNA synthetase, alpha subunit
Synonyms: 0610012A19Rik, Farsla
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 66590
HGNC: HGNC:3592
Homologene: 3280
2610008E11Rik
Name: RIKEN cDNA 2610008E11 gene
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 72128
Homologene: 86705
Ddit4
Name: DNA-damage-inducible transcript 4
Synonyms: Dig2, REDD1, Rtp801, 5830413E08Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 74747
VEGA: 10
Homologene: 10400
Raver2
Name: ribonucleoprotein, PTB-binding 2
Synonyms: A430091O22Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242570
Homologene: 18829
Tbc1d2
Name: TBC1 domain family, member 2
Synonyms: LOC381605, PARIS-1, PARIS1, A630005A06Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 381605
Homologene: 10190
Hpse
Name: heparanase
Synonyms: Hpa
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 15442
HGNC: HGNC:5164
Homologene: 68528
Lrp1
Name: low density lipoprotein receptor-related protein 1
Synonyms: CD91, A2mr, b2b1554Clo
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 16971
HGNC: HGNC:6692
Homologene: 1744
Plet1
Name: placenta expressed transcript 1
Synonyms: 0610037B23Rik, PLET1, 1600029D21Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 76509
Homologene: 49928
Unc13c
Name: unc-13 homolog C
Synonyms: Munc13-3, Unc13h3, D9Ertd414e, 1500037O19Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 208898
Homologene: 45443
Ppfia2
Name: protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 2
Synonyms: E130120L08Rik, Liprin-alpha2
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 327814
VEGA: 10
HGNC: HGNC:9246
Homologene: 27953
Col6a3
Name: collagen, type VI, alpha 3
Synonyms: Col6a-3
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12835
HGNC: HGNC:2213
Homologene: 37917
Arhgef5
Name: Rho guanine nucleotide exchange factor 5
Synonyms: 2210412D05Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 54324
Homologene: 66300
Aox1
Name: aldehyde oxidase 1
Synonyms: retinal oxidase, Aox-1, Aox-2, Aox2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 11761
HGNC: HGNC:553
Homologene: 68165
Ovol2
Name: ovo like zinc finger 2
Synonyms: M-OVO-B, M-OVO-A, M-OVO, 1810007D21Rik, Ovol2, Zfp339, movo2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 107586
Homologene: 10930
Stmnd1
Name: stathmin domain containing 1
Synonyms: LOC380842, Gm1574
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 380842
VEGA: 13
Homologene: 54416
Cts8
Name: cathepsin 8
Synonyms: Epcs70, Epcs68, CTS2
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 56094
VEGA: 13
Homologene: 129897
Igfn1
Name: immunoglobulin-like and fibronectin type III domain containing 1
Synonyms: 9830123M21Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226438
Homologene: 130054
Cdh20
Name: cadherin 20
Synonyms: Cdh7
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 23836
HGNC: HGNC:1760
Homologene: 8015
Tm4sf1
Name: transmembrane 4 superfamily member 1
Synonyms: 12A8 target antigen, L6 antigen, L6, M3s1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 17112
Homologene: 7409
Gm5773
Name: predicted pseudogene 5773
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 436563
Gm1527
Name: predicted gene 1527
Synonyms: LOC385263
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 385263
Homologene: 133976
Kptn
Name: kaptin
Synonyms: 2E4, actin-binding protein, 2310042D10Rik, C030013F01Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 70394
HGNC: HGNC:6404
Homologene: 5127
Slc22a16
Name: solute carrier family 22 (organic cation transporter), member 16
Synonyms: FLIPT2, OKB1, OCT6, CT2, 4921504E14Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 70840
Homologene: 41957
Sult1b1
Name: sulfotransferase family 1B, member 1
Synonyms: Dopa/tyrosine sulfotransferase
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 56362
Homologene: 69169
Zeb1os1
Name: zinc finger E-box binding homeobox 1, opposite strand 1
Synonyms: Zeb1os, Gm10125
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 791318
Lpcat2
Name: lysophosphatidylcholine acyltransferase 2
Synonyms: Aytl1, lysoPAFAT/LPCAT2, LPCAT2, Aytl1a
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 270084
Homologene: 41201
B530045E10Rik
Name: RIKEN cDNA B530045E10 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 118567817
Gnpnat1
Name: glucosamine-phosphate N-acetyltransferase 1
Synonyms: EMeg32
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 54342
VEGA: 14
Homologene: 41323
4933413J09Rik
Name: RIKEN cDNA 4933413J09 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 71106
Pcdhac2
Name: protocadherin alpha subfamily C, 2
Synonyms: CNRc2
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 353237
HGNC: HGNC:8677
Homologene: 49562
RP23-6C10.3
Name:
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Wfdc10
Name: WAP four-disulfide core domain 10
Synonyms: OTTMUSG00000016289
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 629756
Homologene: 86879
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 58,030,026 bp
  • A to G, chromosome 1 at 90,768,345 bp
  • A to G, chromosome 1 at 104,947,348 bp
  • A to G, chromosome 1 at 135,959,896 bp
  • A to T, chromosome 1 at 162,697,846 bp
  • C to T, chromosome 2 at 144,317,860 bp
  • C to T, chromosome 2 at 164,657,140 bp
  • T to A, chromosome 3 at 28,903,418 bp
  • T to G, chromosome 3 at 57,292,868 bp
  • T to A, chromosome 3 at 93,773,428 bp
  • T to C, chromosome 4 at 25,256,010 bp
  • G to A, chromosome 4 at 46,633,639 bp
  • T to C, chromosome 4 at 101,102,985 bp
  • T to A, chromosome 4 at 151,969,814 bp
  • C to T, chromosome 5 at 21,599,268 bp
  • T to G, chromosome 5 at 87,521,548 bp
  • C to A, chromosome 5 at 100,719,537 bp
  • A to T, chromosome 6 at 43,273,214 bp
  • T to A, chromosome 7 at 16,125,785 bp
  • A to G, chromosome 8 at 84,868,964 bp
  • A to T, chromosome 8 at 92,909,191 bp
  • C to T, chromosome 9 at 50,504,295 bp
  • T to C, chromosome 9 at 73,933,372 bp
  • A to T, chromosome 9 at 108,207,572 bp
  • A to G, chromosome 10 at 18,093,880 bp
  • G to A, chromosome 10 at 40,573,957 bp
  • A to G, chromosome 10 at 59,950,669 bp
  • T to C, chromosome 10 at 79,066,992 bp
  • A to T, chromosome 10 at 92,886,387 bp
  • A to G, chromosome 10 at 99,422,121 bp
  • A to G, chromosome 10 at 106,835,760 bp
  • T to A, chromosome 10 at 127,539,634 bp
  • A to G, chromosome 12 at 74,898,084 bp
  • G to A, chromosome 13 at 46,299,595 bp
  • C to T, chromosome 13 at 61,253,335 bp
  • A to T, chromosome 13 at 65,279,033 bp
  • C to G, chromosome 13 at 100,781,775 bp
  • A to G, chromosome 14 at 26,376,307 bp
  • A to G, chromosome 14 at 45,373,504 bp
  • A to G, chromosome 16 at 18,247,287 bp
  • C to T, chromosome 17 at 31,590,739 bp
  • T to C, chromosome 18 at 5,567,390 bp
  • G to T, chromosome 18 at 37,144,299 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5127 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042715-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.