Strain Name:
C57BL/6J-MtgxR5132Btlr/Mmmh
Stock Number:
042720-MU
Citation ID:
RRID:MMRRC_042720-MU
Other Names:
R5132 (G1), C57BL/6J-MtgxR5132Btlr
Major Collection:

Strain Information

Scn3a
Name: sodium channel, voltage-gated, type III, alpha
Synonyms: LOC381367, Nav1.3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 20269
Homologene: 56005
Magi1
Name: membrane associated guanylate kinase, WW and PDZ domain containing 1
Synonyms: WWP3, Gukmi1, AIP3, BAP1, Baiap1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 14924
HGNC: HGNC:946
Homologene: 31257
Serpinb6a
Name: serine (or cysteine) peptidase inhibitor, clade B, member 6a
Synonyms: Spi3, ovalbumin, D330015H01Rik, 4930482L21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 20719
HGNC: HGNC:8950
Homologene: 20956
Nsfl1c
Name: NSFL1 (p97) cofactor (p47)
Synonyms: p47
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 386649
Homologene: 41114
Abl2
Name: v-abl Abelson murine leukemia viral oncogene 2 (arg, Abelson-related gene)
Synonyms: Arg, Abll
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 11352
HGNC: HGNC:77
Homologene: 5278
Rgs12
Name: regulator of G-protein signaling 12
Synonyms: 4632412M04Rik, 1200016K18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 71729
HGNC: HGNC:9994
Homologene: 2195
Prkca
Name: protein kinase C, alpha
Synonyms: Pkca
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 18750
HGNC: HGNC:9393
Homologene: 55679
Drosha
Name: drosha, ribonuclease type III
Synonyms: 1110013A17Rik, Etohi2, Rnasen
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 14000
Homologene: 8293
Ago1
Name: argonaute RISC catalytic subunit 1
Synonyms: argonaute 1, Eif2c1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 236511
HGNC: HGNC:3262
Homologene: 81826
Mtbp
Name: Mdm2, transformed 3T3 cell double minute p53 binding protein
Synonyms: MDM2BP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 105837
HGNC: HGNC:7417
Homologene: 11102
Pspc1
Name: paraspeckle protein 1
Synonyms: 5730470C09Rik, PSP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 66645
Homologene: 10108
St7
Name: suppression of tumorigenicity 7
Synonyms: RAY1, HELG, SEN4, TSG7, Fam4a2, 9430001H04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 64213
Homologene: 10185
Htt
Name: huntingtin
Synonyms: huntingtin, HD, htt, IT15, C430023I11Rik, Hdh
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 15194
HGNC: HGNC:4851
Homologene: 1593
Pdia4
Name: protein disulfide isomerase associated 4
Synonyms: ERp72, Erp72, Cai, U48620
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 12304
Homologene: 21020
Tmem170
Name: transmembrane protein 170
Synonyms: 9030409E16Rik, D8Bwg1414e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 66817
Homologene: 12040
Cdc23
Name: CDC23 cell division cycle 23
Synonyms: D18Ertd243e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 52563
HGNC: HGNC:1724
Homologene: 3426
Timm23
Name: translocase of inner mitochondrial membrane 23
Synonyms: Tim23
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 53600
Homologene: 9651
Smap2
Name: small ArfGAP 2
Synonyms: 1810031K02Rik, Smap2, Smap1l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 69780
Homologene: 41489
Pprc1
Name: peroxisome proliferative activated receptor, gamma, coactivator-related 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 226169
Homologene: 9006
Pomt2
Name: protein-O-mannosyltransferase 2
Synonyms: A830009D15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 217734
VEGA: 12
Homologene: 5297
Pcdh7
Name: protocadherin 7
Synonyms: BH-protocadherin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 54216
HGNC: HGNC:8659
Homologene: 36101
Tgm7
Name: transglutaminase 7
Synonyms: TGz
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 640543
Homologene: 14192
Grik5
Name: glutamate receptor, ionotropic, kainate 5 (gamma 2)
Synonyms: KA2, GluRgamma2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 14809
HGNC: HGNC:4583
Homologene: 1578
Gria1
Name: glutamate receptor, ionotropic, AMPA1 (alpha 1)
Synonyms: GluR1, GluR-A, Glr-1, Glur-1, Glur1, GluRA, Glr1, 2900051M01Rik, HIPA1, GluA1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 14799
HGNC: HGNC:4571
Homologene: 20226
Acad11
Name: acyl-Coenzyme A dehydrogenase family, member 11
Synonyms: 5730439E10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 102632
Homologene: 49896
Cyp2j5
Name: cytochrome P450, family 2, subfamily j, polypeptide 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 13109
HGNC: HGNC:2634
Homologene: 116530
Nsd3
Name: nuclear receptor binding SET domain protein 3
Synonyms: WHISTLE, Whsc1l1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234135
Homologene: 56960
Ndor1
Name: NADPH dependent diflavin oxidoreductase 1
Synonyms: NR1, 4930447P04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 78797
Homologene: 7144
Or5e1
Name: olfactory receptor family 5 subfamily E member 1
Synonyms: GA_x6K02T2PBJ9-11084889-11085818, MOR195-1, Olfr513
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258718
Homologene: 64908
Arhgap26
Name: Rho GTPase activating protein 26
Synonyms: 1810044B20Rik, 2610010G17Rik, 4933432P15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 71302
Homologene: 36349
1110017D15Rik
Name: RIKEN cDNA 1110017D15 gene
Synonyms: Smrp1, Cbe1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 73721
Homologene: 13074
Nostrin
Name: nitric oxide synthase trafficker
Synonyms: mDaIP2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 329416
Homologene: 43142
Or10ag56
Name: olfactory receptor family 10 subfamily AG member 56
Synonyms: GA_x6K02T2Q125-48795705-48796673, MOR264-22, Olfr1118-ps, Olfr1118
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 404328
Homologene: 134077
Ddx19b
Name: DEAD box helicase 19b
Synonyms: 4921519L13Rik, 2810457M08Rik, DEAD (Asp-Glu-Ala-Asp) box polypeptide 19b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234733
HGNC: HGNC:2742
Homologene: 56032
Or4k49
Name: olfactory receptor family 4 subfamily K member 49
Synonyms: GA_x6K02T2Q125-72715642-72716580, MOR248-8, Olfr1299
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258886
Homologene: 17426
Pafah1b3
Name: platelet-activating factor acetylhydrolase, isoform 1b, subunit 3
Synonyms: mus[g], Pafahg
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 18476
HGNC: HGNC:8576
Homologene: 1933
Cbx8
Name: chromobox 8
Synonyms: polycomb 3, Pc3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 30951
Homologene: 32199
Cdc42ep3
Name: CDC42 effector protein (Rho GTPase binding) 3
Synonyms: UB1, Cep3, Borg2, 3200001F04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 260409
VEGA: 17
Homologene: 4708
Gpr25
Name: G protein-coupled receptor 25
Synonyms: LOC383563
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 383563
HGNC: HGNC:4480
Homologene: 3872
Larp6
Name: La ribonucleoprotein 6, translational regulator
Synonyms: 5430431G03Rik, Achn, acheron
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 67557
Homologene: 32408
Bach2os
Name: BTB and CNC homology 2, opposite strand
Synonyms: BC024582
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 414068
Gm3835
Name: predicted pseudogene 3835
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Gm8888
Name: predicted gene 8888
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
VEGA: 15
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 136,260,365 bp
  • A to T, chromosome 1 at 156,641,832 bp
  • A to T, chromosome 2 at 25,247,769 bp
  • C to T, chromosome 2 at 65,468,204 bp
  • G to A, chromosome 2 at 69,183,883 bp
  • A to T, chromosome 2 at 87,308,938 bp
  • T to A, chromosome 2 at 111,664,999 bp
  • A to G, chromosome 2 at 121,104,219 bp
  • C to T, chromosome 2 at 151,506,295 bp
  • A to G, chromosome 4 at 32,563,396 bp
  • A to G, chromosome 4 at 41,517,178 bp
  • T to C, chromosome 4 at 96,629,496 bp
  • T to C, chromosome 4 at 120,973,173 bp
  • T to C, chromosome 4 at 126,461,723 bp
  • T to C, chromosome 5 at 34,905,679 bp
  • A to G, chromosome 5 at 34,989,812 bp
  • T to C, chromosome 5 at 57,728,121 bp
  • A to T, chromosome 6 at 17,854,957 bp
  • A to G, chromosome 6 at 47,796,735 bp
  • C to A, chromosome 6 at 93,683,091 bp
  • C to T, chromosome 7 at 25,065,204 bp
  • T to C, chromosome 7 at 25,295,179 bp
  • T to C, chromosome 7 at 108,755,270 bp
  • A to G, chromosome 8 at 25,678,839 bp
  • C to T, chromosome 8 at 72,431,368 bp
  • A to T, chromosome 8 at 111,022,408 bp
  • A to T, chromosome 8 at 111,869,725 bp
  • A to G, chromosome 9 at 60,737,210 bp
  • A to T, chromosome 9 at 104,126,592 bp
  • A to G, chromosome 11 at 57,289,399 bp
  • T to C, chromosome 11 at 108,192,117 bp
  • G to T, chromosome 11 at 119,039,511 bp
  • A to G, chromosome 12 at 87,110,347 bp
  • C to T, chromosome 13 at 33,918,322 bp
  • A to T, chromosome 14 at 32,193,945 bp
  • A to T, chromosome 14 at 56,723,250 bp
  • A to G, chromosome 15 at 12,837,291 bp
  • A to G, chromosome 15 at 55,558,569 bp
  • A to G, chromosome 15 at 96,767,011 bp
  • C to T, chromosome 17 at 79,335,374 bp
  • C to A, chromosome 18 at 34,651,689 bp
  • A to G, chromosome 18 at 39,126,876 bp
  • T to C, chromosome 19 at 46,072,682 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5132 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042720-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.