Strain Name:
C57BL/6J-MtgxR5144Btlr/Mmmh
Stock Number:
042728-MU
Citation ID:
RRID:MMRRC_042728-MU
Other Names:
R5144 (G1), C57BL/6J-MtgxR5144Btlr
Major Collection:

Strain Information

Ncor1
Name: nuclear receptor co-repressor 1
Synonyms: N-CoR, Rxrip13, A230020K14Rik, 5730405M06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 20185
HGNC: HGNC:7672
Homologene: 38166
B4galt5
Name: UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 5
Synonyms: 9430078I07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 56336
HGNC: HGNC:928
Homologene: 3507
Nlgn3
Name: neuroligin 3
Synonyms: HNL3, A230085M13Rik, NL3, NLG3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 245537
Homologene: 23133
Chrna4
Name: cholinergic receptor, nicotinic, alpha polypeptide 4
Synonyms: alpha4 nAChR, Acra-4, Acra4, a4 nicotinic receptor, alpha4-nAChR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 11438
HGNC: HGNC:1958
Homologene: 592
Depdc7
Name: DEP domain containing 7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 211896
Homologene: 16386
Pik3c2a
Name: phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 alpha
Synonyms: PI3KC2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 18704
HGNC: HGNC:8971
Homologene: 20581
Tcf3
Name: transcription factor 3
Synonyms: ALF2, E2A, E47, E12, Pan2, Pan1, A1, Tcfe2a, bHLHb21
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 21423
Homologene: 2408
Prmt3
Name: protein arginine N-methyltransferase 3
Synonyms: 2410018A17Rik, 2010005E20Rik, Hrmt1l3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 71974
Homologene: 24255
Arpc2
Name: actin related protein 2/3 complex, subunit 2
Synonyms: 2210023N03Rik, p34-Arc
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 76709
HGNC: HGNC:705
Homologene: 4187
Eif3c
Name: eukaryotic translation initiation factor 3, subunit C
Synonyms: NIPIL(A3), 110kDa, 3230401O13Rik, Eif3s8, Xsl, Xs
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 56347
Homologene: 2781
Cep350
Name: centrosomal protein 350
Synonyms: 6430546F08Rik, 4933409L06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 74081
Homologene: 8879
Vps8
Name: VPS8 CORVET complex subunit
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 209018
Homologene: 44592
Rgs2
Name: regulator of G-protein signaling 2
Synonyms: GOS8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 19735
HGNC: HGNC:9998
Homologene: 2192
Zdhhc6
Name: zinc finger, DHHC domain containing 6
Synonyms: 5930409M18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 66980
VEGA: 19
Homologene: 41487
Epg5
Name: ectopic P-granules autophagy protein 5 homolog (C. elegans)
Synonyms: 5430411K18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 100502841
VEGA: 18
Homologene: 14575
Plppr1
Name: phospholipid phosphatase related 1
Synonyms: PRG-3, E130309F12Rik, Lppr1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 272031
Homologene: 9815
Ccdc71
Name: coiled-coil domain containing 71
Synonyms: 2600016J21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 72454
Homologene: 11288
Kcnj3
Name: potassium inwardly-rectifying channel, subfamily J, member 3
Synonyms: Kir3.1, GIRK1, Kcnf3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 16519
HGNC: HGNC:6264
Homologene: 1687
Bub1b
Name: BUB1B, mitotic checkpoint serine/threonine kinase
Synonyms: BUBR1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 12236
HGNC: HGNC:1149
Homologene: 933
Tgm3
Name: transglutaminase 3, E polypeptide
Synonyms: TG E, we
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 21818
Homologene: 20690
Zdhhc25
Name: zinc finger, DHHC domain containing 25
Synonyms: 1700030J15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 70073
Homologene: 19298
Ect2l
Name: epithelial cell transforming sequence 2 oncogene-like
Synonyms: Gm10331, C330021H03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 100045792
Homologene: 46005
Stk35
Name: serine/threonine kinase 35
Synonyms: CLP-36 interacting kinase, CLIK1, 1700054C12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 67333
Homologene: 15441
Dppa2
Name: developmental pluripotency associated 2
Synonyms: 2410088E07Rik, ECAT15-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 73703
VEGA: 16
Homologene: 79572
Slc2a8
Name: solute carrier family 2, (facilitated glucose transporter), member 8
Synonyms: GLUT8, GlutX1, D2Ertd44e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 56017
Homologene: 69170
Ino80c
Name: INO80 complex subunit C
Synonyms: D030070L09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 225280
Homologene: 45426
Col9a1
Name: collagen, type IX, alpha 1
Synonyms: Col9a-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12839
HGNC: HGNC:2217
Homologene: 1393
Col6a5
Name: collagen, type VI, alpha 5
Synonyms: Col6a5, Gm7455
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 665033
Homologene: 122792
Pappa2
Name: pappalysin 2
Synonyms: placenta-specific 3, pregnancy-associated plasma preproprotein-A2, pregnancy-associated plasma protein-E, PAPP-A2, PLAC3, Pappe
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 23850
Homologene: 10661
Kcnb1
Name: potassium voltage gated channel, Shab-related subfamily, member 1
Synonyms: Kv2.1, Kcr1-1, Shab
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 16500
HGNC: HGNC:6231
Homologene: 37988
Or8b53
Name: olfactory receptor family 8 subfamily B member 53
Synonyms: GA_x6K02T2PVTD-32458442-32459374, MOR165-6, Olfr920
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 258783
VEGA: 9
4933409G03Rik
Name: RIKEN cDNA 4933409G03 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 227998
Nudt19
Name: nudix hydrolase 19
Synonyms: androgen regulated gene RP2, D7Rp2-s, D7Rp2-r, D7Rp2, RP2-s, RP2-r, D7RP2e, nudix (nucleoside diphosphate linked moiety X)-type motif 19
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 110959
Homologene: 13211
Prm2
Name: protamine 2
Synonyms: Prm-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 19119
HGNC: HGNC:9448
Wipf3
Name: WAS/WASL interacting protein family, member 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 330319
Homologene: 113391
Rars2
Name: arginyl-tRNA synthetase 2, mitochondrial
Synonyms: PRO1992, 1500002I10Rik, Rarsl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 109093
Homologene: 5707
Nod2
Name: nucleotide-binding oligomerization domain containing 2
Synonyms: F830032C23Rik, Card15, Nlrc2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 257632
HGNC: HGNC:5331
Homologene: 11156
Or1j4
Name: olfactory receptor family 1 subfamily J member 4
Synonyms: GA_x6K02T2NLDC-33544602-33545540, MOR136-13, Olfr350
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258620
HGNC: HGNC:8211
Homologene: 64897
Vmn1r237
Name: vomeronasal 1 receptor 237
Synonyms: V1rf3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 171234
Homologene: 64475
Utp11
Name: UTP11 small subunit processome component
Synonyms: 2700082D03Rik, Utp11l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 67205
Homologene: 6349
Tssc4
Name: tumor-suppressing subchromosomal transferable fragment 4
Synonyms: ESTM671070
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 56844
Homologene: 4169
Pdcd5
Name: programmed cell death 5
Synonyms: Tfar19, 2200003D22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 56330
HGNC: HGNC:8764
Homologene: 10506
Prdm6
Name: PR domain containing 6
Synonyms: LOC225518, PRISM
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 225518
HGNC: HGNC:9350
Homologene: 79756
Vmn2r129
Name: vomeronasal 2, receptor 129
Synonyms: V2r8, V2r9, Vmn2r-ps160, Vmn2r-ps159
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 22314
Gm13883
Name: predicted gene 13883
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 118568544
Gm29331
Name: predicted gene 29331
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 24,239,353 bp
  • A to T, chromosome 1 at 74,248,208 bp
  • G to A, chromosome 1 at 144,001,699 bp
  • G to A, chromosome 1 at 155,911,150 bp
  • T to A, chromosome 1 at 158,957,133 bp
  • C to T, chromosome 2 at 32,981,773 bp
  • A to T, chromosome 2 at 36,850,144 bp
  • G to A, chromosome 2 at 55,447,047 bp
  • T to A, chromosome 2 at 68,616,260 bp
  • T to C, chromosome 2 at 104,258,502 bp
  • T to C, chromosome 2 at 104,730,253 bp
  • C to T, chromosome 2 at 118,615,499 bp
  • T to A, chromosome 2 at 129,810,935 bp
  • C to T, chromosome 2 at 130,048,282 bp
  • T to A, chromosome 2 at 167,105,944 bp
  • C to T, chromosome 2 at 167,306,596 bp
  • A to G, chromosome 2 at 181,024,830 bp
  • T to C, chromosome 4 at 34,656,793 bp
  • T to A, chromosome 4 at 49,319,800 bp
  • A to T, chromosome 4 at 124,678,902 bp
  • G to T, chromosome 4 at 156,334,397 bp
  • C to T, chromosome 6 at 54,485,675 bp
  • T to C, chromosome 7 at 35,555,225 bp
  • T to C, chromosome 7 at 35,642,642 bp
  • T to C, chromosome 7 at 49,786,135 bp
  • T to C, chromosome 7 at 116,350,786 bp
  • T to C, chromosome 7 at 126,563,066 bp
  • T to G, chromosome 7 at 143,070,033 bp
  • T to C, chromosome 8 at 88,653,066 bp
  • C to T, chromosome 9 at 38,756,393 bp
  • T to A, chromosome 9 at 105,889,283 bp
  • T to C, chromosome 9 at 108,463,852 bp
  • A to T, chromosome 10 at 18,144,577 bp
  • G to A, chromosome 10 at 80,415,237 bp
  • T to G, chromosome 11 at 62,349,464 bp
  • T to G, chromosome 15 at 88,601,056 bp
  • C to A, chromosome 15 at 98,585,267 bp
  • T to A, chromosome 16 at 10,791,868 bp
  • T to C, chromosome 16 at 21,559,353 bp
  • T to C, chromosome 16 at 48,317,303 bp
  • C to G, chromosome 17 at 21,314,426 bp
  • T to A, chromosome 18 at 24,108,878 bp
  • C to A, chromosome 18 at 53,465,038 bp
  • T to C, chromosome 18 at 78,015,680 bp
  • A to T, chromosome 19 at 55,314,566 bp
  • G to A, chromosome X at 101,318,285 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5144 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042728-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.