Strain Name:
C57BL/6J-MtgxR5145Btlr/Mmmh
Stock Number:
042729-MU
Citation ID:
RRID:MMRRC_042729-MU
Other Names:
R5145 (G1), C57BL/6J-MtgxR5145Btlr
Major Collection:

Gene Information

Nfatc2
Name: nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 2
Synonyms: NFAT1, NFAT1-D, NFATp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 18019
HGNC: HGNC:7776
Homologene: 7861
Sema3c
Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C
Synonyms: 1110036B02Rik, Semae
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 20348
Homologene: 36201
Nlgn3
Name: neuroligin 3
Synonyms: NLG3, A230085M13Rik, NL3, HNL3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 245537
Homologene: 23133
Tti1
Name: TELO2 interacting protein 1
Synonyms: 2610036D13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 75425
Homologene: 40969
Lin9
Name: lin-9 homolog (C. elegans)
Synonyms: 2700022J23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 72568
Homologene: 35252
Fry
Name: FRY microtubule binding protein
Synonyms: cg003, 9330186A19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 320365
Homologene: 113770
Ankrd11
Name: ankyrin repeat domain 11
Synonyms: 3010027A04Rik, 2410104C19Rik, 9530048I21Rik, Yod
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 77087
Homologene: 69134
Ranbp2
Name: RAN binding protein 2
Synonyms: A430087B05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 19386
VEGA: 10
Homologene: 87808
Ptprc
Name: protein tyrosine phosphatase, receptor type, C
Synonyms: CD45, Lyt-4, Ly-5, T200, B220
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 19264
HGNC: HGNC:9666
Homologene: 2126
Rnf167
Name: ring finger protein 167
Synonyms: 0610010G05Rik, 5730408C10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 70510
Homologene: 41046
Adamts12
Name: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 239337
VEGA: 15
Homologene: 12808
Rbl1
Name: RB transcriptional corepressor like 1
Synonyms: retinoblastoma-like 1 (p107), p107
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 19650
HGNC: HGNC:9893
Homologene: 2172
Pum3
Name: pumilio RNA-binding family member 3
Synonyms: D19Bwg1357e, 1110069H02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 52874
VEGA: 19
Homologene: 5762
Tia1
Name: cytotoxic granule-associated RNA binding protein 1
Synonyms: mTIA-1, 2310050N03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 21841
Homologene: 20692
Ddx21
Name: DExD box helicase 21
Synonyms: D10Ertd645e, RH-II/Gualpha, DEAD (Asp-Glu-Ala-Asp) box polypeptide 21, RH II/Gu, D10Wsu42e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 56200
VEGA: 10
HGNC: HGNC:2744
Homologene: 3473
Mfng
Name: MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
Synonyms: manic fringe
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 17305
HGNC: HGNC:7038
Homologene: 1803
Col12a1
Name: collagen, type XII, alpha 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 12816
HGNC: HGNC:2188
Homologene: 3217
Tdrd6
Name: tudor domain containing 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 210510
Homologene: 19364
Vmn2r10
Name: vomeronasal 2, receptor 10
Synonyms: V2r16, VR16
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 22307
Homologene: 129606
Eif2ak2
Name: eukaryotic translation initiation factor 2-alpha kinase 2
Synonyms: 2310047A08Rik, IFN-induced and double-stranded RNA-activated kinase, 4732414G15Rik, eIF-2 alpha, IFN- type I-induced and dsRNA-activated kinase, dsRNA-activated kinase, Prkr, Tik, eIF-2 alpha, Pkr
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 19106
HGNC: HGNC:9437
Homologene: 48134
Ccdc39
Name: coiled-coil domain containing 39
Synonyms: b2b2025.1Clo, D3Ertd789e, 4921507O14Rik, b2b1735Clo, b2b1304Clo, prh
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 51938
Homologene: 12149
Sdr9c7
Name: 4short chain dehydrogenase/reductase family 9C, member 7
Synonyms: 1810054F20Rik, Rdhs, Sdro, Rdh20, SDR-O
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 70061
Homologene: 23618
Col6a5
Name: collagen, type VI, alpha 5
Synonyms: Gm7455, Col6a5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 665033
Homologene: 122792
Mfsd2b
Name: major facilitator superfamily domain containing 2B
Synonyms: Gm1964
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 432628
Homologene: 47753
Olfr1352
Name: olfactory receptor 1352
Synonyms: MOR139-1, GA_x6K02T2QGN0-2828447-2827518
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 259074
HGNC: HGNC:8373
Homologene: 131141
Efcab12
Name: EF-hand calcium binding domain 12
Synonyms: BC060267
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 212516
Homologene: 18905
Ptprb
Name: protein tyrosine phosphatase, receptor type, B
Synonyms: VE-PTP, 3230402H02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 19263
VEGA: 10
HGNC: HGNC:9665
Homologene: 2125
Stt3a
Name: STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae)
Synonyms: Itm1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 16430
HGNC: HGNC:6172
Homologene: 40617
Tha1
Name: threonine aldolase 1
Synonyms: 1300017K07Rik, GLY1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 71776
Homologene: 57081
Gm9772
Name: predicted gene 9772
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 100416807
VEGA: 17
Npy6r
Name: neuropeptide Y receptor Y6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 18169
VEGA: 18
HGNC: HGNC:7959
Homologene: 134692
Adgrg7
Name: adhesion G protein-coupled receptor G7
Synonyms: Gpr128, 9130020O16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 239853
Homologene: 13115
Cry2
Name: cryptochrome 2 (photolyase-like)
Synonyms: D130054K12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 12953
HGNC: HGNC:2385
Homologene: 56466
Meig1
Name: meiosis expressed gene 1
Synonyms: Meg1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 104362
Homologene: 56414
Gm20997
Name: predicted gene, 20997
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
RP23-227D7.10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 138,089,566 bp
  • A to G, chromosome 1 at 180,661,573 bp
  • T to C, chromosome 2 at 3,409,226 bp
  • T to C, chromosome 2 at 92,413,060 bp
  • A to T, chromosome 2 at 157,175,477 bp
  • G to A, chromosome 2 at 158,008,512 bp
  • A to C, chromosome 2 at 168,590,067 bp
  • C to T, chromosome 3 at 33,813,155 bp
  • A to G, chromosome 5 at 17,727,617 bp
  • A to G, chromosome 5 at 87,486,027 bp
  • T to C, chromosome 5 at 108,995,895 bp
  • T to C, chromosome 5 at 150,370,224 bp
  • A to G, chromosome 6 at 86,425,009 bp
  • T to A, chromosome 6 at 115,823,277 bp
  • G to A, chromosome 6 at 130,483,768 bp
  • A to G, chromosome 8 at 122,891,204 bp
  • T to C, chromosome 9 at 36,735,466 bp
  • T to C, chromosome 9 at 79,706,300 bp
  • T to G, chromosome 9 at 105,934,245 bp
  • T to A, chromosome 10 at 58,480,038 bp
  • T to C, chromosome 10 at 62,587,539 bp
  • A to G, chromosome 10 at 78,984,309 bp
  • A to G, chromosome 10 at 116,343,915 bp
  • T to C, chromosome 10 at 127,902,390 bp
  • T to C, chromosome 11 at 70,650,080 bp
  • A to C, chromosome 11 at 117,869,676 bp
  • T to A, chromosome 12 at 4,865,908 bp
  • G to A, chromosome 15 at 11,285,876 bp
  • C to T, chromosome 15 at 78,764,388 bp
  • T to A, chromosome 16 at 56,742,319 bp
  • C to T, chromosome 17 at 22,007,126 bp
  • T to A, chromosome 17 at 43,626,075 bp
  • T to C, chromosome 17 at 78,876,204 bp
  • C to T, chromosome 18 at 44,276,619 bp
  • A to G, chromosome 19 at 27,399,769 bp
  • G to A, chromosome X at 101,318,285 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5145 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042729-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.