Strain Name:
C57BL/6J-MtgxR5146Btlr/Mmmh
Stock Number:
042730-MU
Citation ID:
RRID:MMRRC_042730-MU
Other Names:
R5146 (G1), C57BL/6J-MtgxR5146Btlr
Major Collection:

Gene Information

Nlgn3
Name: neuroligin 3
Synonyms: NLG3, A230085M13Rik, HNL3, NL3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Chemically Induced
NCBI: 245537
VEGA: X
Homologene: 23133
Rnf20
Name: ring finger protein 20
Synonyms: 4833430L21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 109331
VEGA: 4
Homologene: 5571
Dock4
Name: dedicator of cytokinesis 4
Synonyms: EST N28122, 6330411N01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 238130
VEGA: 12
Homologene: 56680
Pot1b
Name: protection of telomeres 1B
Synonyms: 2810458H16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 72836
VEGA: 17
Homologene: 87058
Sumf1
Name: sulfatase modifying factor 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 58911
VEGA: 6
Homologene: 16268
Pirb
Name: paired Ig-like receptor B
Synonyms: Lilrb3, Gp91
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 18733
VEGA: 7
HGNC: HGNC:6607
Homologene: 134028
Cspp1
Name: centrosome and spindle pole associated protein 1
Synonyms: 2310020J12Rik, 4930413O22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 211660
VEGA: 1
Homologene: 23487
Itfg1
Name: integrin alpha FG-GAP repeat containing 1
Synonyms: D8Wsu49e, 2310047C21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 71927
VEGA: 8
Homologene: 12423
2700049A03Rik
Name: RIKEN cDNA 2700049A03 gene
Synonyms: talpid3, Ta3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 76967
VEGA: 12
Homologene: 8839
Grwd1
Name: glutamate-rich WD repeat containing 1
Synonyms: WDR28
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 101612
VEGA: 7
Homologene: 6644
Mfng
Name: MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
Synonyms: manic fringe
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 17305
VEGA: 15
HGNC: HGNC:7038
Homologene: 1803
Csmd1
Name: CUB and Sushi multiple domains 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 94109
VEGA: 8
Homologene: 69536
Fgfr4
Name: fibroblast growth factor receptor 4
Synonyms: Fgfr-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 14186
VEGA: 13
HGNC: HGNC:3691
Homologene: 20461
Ttn
Name: titin
Synonyms: shru, connectin, mdm, 2310057K23Rik, D330041I19Rik, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, L56, 2310074I15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 22138
VEGA: 2
Homologene: 130650
Cntnap5c
Name: contactin associated protein-like 5C
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 620292
VEGA: 17
Homologene: 128806
Dnah17
Name: dynein, axonemal, heavy chain 17
Synonyms: LOC382552, 2810003K23Rik, Dnahcl1, Dnahc17
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 69926
VEGA: 11
HGNC: HGNC:2946
Homologene: 72102
Carmil3
Name: capping protein regulator and myosin 1 linker 3
Synonyms: Lrrc16b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 268747
VEGA: 14
Homologene: 74564
Ahnak2
Name: AHNAK nucleoprotein 2
Synonyms: LOC382643
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 100041194
VEGA: 12
Homologene: 131081
Cdh7
Name: cadherin 7, type 2
Synonyms: CDH7L1, 9330156F07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 241201
VEGA: 1
HGNC: HGNC:1766
Homologene: 68391
Grin2b
Name: glutamate receptor, ionotropic, NMDA2B (epsilon 2)
Synonyms: Nmdar2b, GluN2B, NR2B, NMDAR2B, GluRepsilon2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 14812
VEGA: 6
HGNC: HGNC:4586
Homologene: 646
Vmn2r84
Name: vomeronasal 2, receptor 84
Synonyms: EG625068
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 625068
VEGA: 10
Homologene: 129606
Myo15b
Name: myosin XVB
Synonyms: LOC380737, LOC217328, E330039G21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 217328
VEGA: 11
Kcna2
Name: potassium voltage-gated channel, shaker-related subfamily, member 2
Synonyms: Akr6a4, Mk-2, Kca1-2, Kv1.2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 16490
VEGA: 3
HGNC: HGNC:6220
Homologene: 21034
Adcyap1r1
Name: adenylate cyclase activating polypeptide 1 receptor 1
Synonyms: PAC1R, PACAP1-R, 2900024I10Rik, PAC1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 11517
VEGA: 6
HGNC: HGNC:242
Homologene: 870
Gm7030
Name: predicted gene 7030
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 630294
VEGA: 17
HGNC: HGNC:4963
Homologene: 133121
Tmem101
Name: transmembrane protein 101
Synonyms: 2610511E22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 76547
VEGA: 11
Homologene: 12649
Gpam
Name: glycerol-3-phosphate acyltransferase, mitochondrial
Synonyms: GPAT1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 14732
VEGA: 19
Homologene: 7343
Oas1c
Name: 2'-5' oligoadenylate synthetase 1C
Synonyms: Oasl5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 114643
VEGA: 5
HGNC: HGNC:8086
Homologene: 110815
Zfp873
Name: zinc finger protein 873
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 408062
VEGA: 10
Homologene: 134319
Chil4
Name: chitinase-like 4
Synonyms: Ym2, Chi3l4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 104183
VEGA: 3
Homologene: 74931
Sppl2b
Name: signal peptide peptidase like 2B
Synonyms: 3110056O03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 73218
VEGA: 10
Homologene: 10605
Gm14415
Name: predicted gene 14415
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 628239
VEGA: 2
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 10,074,876 bp
  • A to G, chromosome 1 at 109,994,312 bp
  • G to A, chromosome 2 at 76,870,363 bp
  • T to C, chromosome 2 at 177,104,231 bp
  • T to A, chromosome 3 at 106,202,834 bp
  • T to C, chromosome 3 at 107,105,498 bp
  • A to T, chromosome 4 at 49,651,456 bp
  • A to G, chromosome 5 at 120,802,094 bp
  • A to G, chromosome 6 at 55,484,972 bp
  • G to A, chromosome 6 at 108,185,310 bp
  • T to A, chromosome 6 at 135,779,342 bp
  • T to C, chromosome 7 at 3,712,621 bp
  • A to T, chromosome 7 at 45,827,834 bp
  • T to C, chromosome 8 at 16,196,190 bp
  • T to C, chromosome 8 at 85,718,868 bp
  • T to C, chromosome 10 at 80,867,640 bp
  • A to G, chromosome 10 at 82,060,224 bp
  • A to G, chromosome 10 at 130,386,102 bp
  • C to T, chromosome 11 at 102,154,624 bp
  • A to G, chromosome 11 at 115,891,198 bp
  • A to G, chromosome 11 at 118,114,179 bp
  • A to G, chromosome 12 at 40,649,492 bp
  • A to G, chromosome 12 at 71,243,025 bp
  • A to C, chromosome 12 at 112,775,726 bp
  • G to T, chromosome 13 at 55,165,912 bp
  • A to G, chromosome 14 at 55,497,179 bp
  • C to T, chromosome 15 at 78,764,388 bp
  • A to G, chromosome 17 at 36,129,015 bp
  • A to T, chromosome 17 at 55,672,865 bp
  • T to C, chromosome 17 at 58,013,847 bp
  • A to G, chromosome 19 at 55,093,946 bp
  • G to A, chromosome X at 101,318,285 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5146 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042730-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.