Strain Name:
C57BL/6J-MtgxR5149Btlr/Mmmh
Stock Number:
042732-MU
Citation ID:
RRID:MMRRC_042732-MU
Other Names:
R5149 (G1), C57BL/6J-MtgxR5149Btlr
Major Collection:

Gene Information

Septin4
Name: septin 4
Synonyms: Pnutl2, Sept4, ARTS, septin H5, Bh5, cell division control-related protein 2b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 18952
HGNC: HGNC:9165
Homologene: 6107
Sparcl1
Name: SPARC-like 1
Synonyms: mast9, Ecm2, hevin, Sc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 13602
Homologene: 3438
Zdbf2
Name: zinc finger, DBF-type containing 2
Synonyms: 4930431J08Rik, 9330107J05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 73884
Homologene: 52868
Trp53bp1
Name: transformation related protein 53 binding protein 1
Synonyms: p53BP1, 53BP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 27223
Homologene: 4137
Helb
Name: helicase (DNA) B
Synonyms: D10Ertd664e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 117599
Homologene: 50463
Gtf3c1
Name: general transcription factor III C 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233863
HGNC: HGNC:4664
Homologene: 31040
Amt
Name: aminomethyltransferase
Synonyms: EG434437
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 434437
HGNC: HGNC:473
Homologene: 409
Tep1
Name: telomerase associated protein 1
Synonyms: Tp1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 21745
VEGA: 14
Homologene: 5157
Trio
Name: triple functional domain (PTPRF interacting)
Synonyms: Solo, 6720464I07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 223435
VEGA: 15
Homologene: 20847
Lrig1
Name: leucine-rich repeats and immunoglobulin-like domains 1
Synonyms: Img, LIG-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 16206
Homologene: 7380
Prox1
Name: prospero homeobox 1
Synonyms: A230003G05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 19130
HGNC: HGNC:9459
Homologene: 2069
Grhl1
Name: grainyhead like transcription factor 1
Synonyms: p61 MGR, LBP-32, Tcfcp2l2, p70 MGR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 195733
VEGA: 12
Homologene: 32219
Klrb1c
Name: killer cell lectin-like receptor subfamily B member 1C
Synonyms: NKR-P1, NKR-P1C, Nk-1.2, NK-RP1, NK-1.1, CD161, Nk-1, Nk1, Ly55c, Nk1.1, Ly59, Ly-59, Nkrp1-c
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 17059
HGNC: HGNC:6373
Homologene: 84369
Larp7
Name: La ribonucleoprotein domain family, member 7
Synonyms: C330027G06Rik, D3Wsu161e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 28036
Homologene: 88843
Snf8
Name: SNF8, ESCRT-II complex subunit, homolog (S. cerevisiae)
Synonyms: D11Moh34
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 27681
Homologene: 5239
Ankrd26
Name: ankyrin repeat domain 26
Synonyms: 5730521P14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232339
Homologene: 45968
Marchf6
Name: membrane associated ring-CH-type finger 6
Synonyms: F830029L24Rik, March6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 223455
VEGA: 15
Homologene: 4301
Papln
Name: papilin, proteoglycan-like sulfated glycoprotein
Synonyms: E030033C16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 170721
Homologene: 71541
Epha5
Name: Eph receptor A5
Synonyms: Ehk1, Hek7, bsk, Rek7, Cek7, Els1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 13839
HGNC: HGNC:3389
Homologene: 55824
Reln
Name: reelin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 19699
HGNC: HGNC:9957
Homologene: 3699
Dchs1
Name: dachsous cadherin related 1
Synonyms: 3110041P15Rik, C130033F22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233651
Homologene: 2771
Nlgn2
Name: neuroligin 2
Synonyms: NLG2, NL2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 216856
Homologene: 69317
Spta1
Name: spectrin alpha, erythrocytic 1
Synonyms: Spna1, erythroid, Spna-1, ihj
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 20739
Homologene: 74460
Dnah12
Name: dynein, axonemal, heavy chain 12
Synonyms: HL19, LOC380889, DHC3, HL-19, 4921531P07Rik, Dnahc12, Hdhc3, DLP12, Dnahc7l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 110083
HGNC: HGNC:2943
Homologene: 56821
Col27a1
Name: collagen, type XXVII, alpha 1
Synonyms: 5730512J02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 373864
Homologene: 69400
Serpinb6e
Name: serine (or cysteine) peptidase inhibitor, clade B, member 6e
Synonyms: ovalbumin, SPI3B, Gm11396
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 435350
HGNC: HGNC:8950
Homologene: 50933
Poteg
Name: POTE ankyrin domain family, member G
Synonyms: 4921537P18Rik, 4930456F22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 70952
Homologene: 134158
Atp1a4
Name: ATPase, Na+/K+ transporting, alpha 4 polypeptide
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 27222
Homologene: 113769
Akr1c13
Name: aldo-keto reductase family 1, member C13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 27384
Homologene: 121208
Stk-ps2
Name: serine/threonine kinase 2
Synonyms: Gm4776
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 212225
Olfr804
Name: olfactory receptor 804
Synonyms: GA_x6K02T2PULF-11383575-11384519, MOR110-7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 258068
Homologene: 133581
Cyp2j5
Name: cytochrome P450, family 2, subfamily j, polypeptide 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 13109
Homologene: 116530
Tnfrsf8
Name: tumor necrosis factor receptor superfamily, member 8
Synonyms: CD30
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 21941
Homologene: 949
Dpep1
Name: dipeptidase 1
Synonyms: MBD
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 13479
HGNC: HGNC:3002
Homologene: 80192
Olfr135
Name: olfactory receptor 135
Synonyms: MOR256-48, GA_x6K02T2PSCP-2656648-2657586
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 258329
Homologene: 110603
Ahsg
Name: alpha-2-HS-glycoprotein
Synonyms: fetuin-A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 11625
VEGA: 16
HGNC: HGNC:349
Homologene: 1225
Slc22a19
Name: solute carrier family 22 (organic anion transporter), member 19
Synonyms: D630043A20Rik, Slc22a9, Oat5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 207151
Homologene: 133125
Mgst2
Name: microsomal glutathione S-transferase 2
Synonyms: MGST-II, GST2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 211666
HGNC: HGNC:7063
Homologene: 1811
Slc22a20
Name: solute carrier family 22 (organic anion transporter), member 20
Synonyms: LOC381203, mOAT6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 381203
Homologene: 87203
D6Ertd527e
Name: DNA segment, Chr 6, ERATO Doi 527, expressed
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 52372
Olfr1138
Name: olfactory receptor 1138
Synonyms: MOR177-8, GA_x6K02T2Q125-49242149-49241214
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258632
Homologene: 74082
Tmem132b
Name: transmembrane protein 132B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 208151
Homologene: 28135
Tat
Name: tyrosine aminotransferase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234724
Homologene: 37293
Acss2
Name: acyl-CoA synthetase short-chain family member 2
Synonyms: AceCS1, acetyl-CoA synthetase 1, Acas2, Acs1, Acas1, ACAS
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 60525
Homologene: 6469
Tspan13
Name: tetraspanin 13
Synonyms: 1100001I23Rik, Tm4sf13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 66109
Homologene: 8671
Gm3409
Name: predicted gene 3409
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100041566
Homologene: 86127
Zfp213
Name: zinc finger protein 213
Synonyms: D17Ertd197e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 449521
VEGA: 17
Homologene: 55809
Ighv14-3
Name: immunoglobulin heavy variable V14-3
Synonyms: Gm16840
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 238418
Hba-ps4
Name: hemoglobin alpha, pseudogene 4
Synonyms: Hba-4ps, Hba-a4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 383229
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 46,029,298 bp
  • T to C, chromosome 1 at 63,304,903 bp
  • A to G, chromosome 1 at 172,232,005 bp
  • A to G, chromosome 1 at 174,247,434 bp
  • T to A, chromosome 1 at 190,147,053 bp
  • A to T, chromosome 2 at 87,737,405 bp
  • A to T, chromosome 2 at 121,216,117 bp
  • C to T, chromosome 2 at 155,549,322 bp
  • A to G, chromosome 3 at 51,682,537 bp
  • C to T, chromosome 3 at 127,540,811 bp
  • T to C, chromosome 4 at 63,331,427 bp
  • A to G, chromosome 4 at 96,659,507 bp
  • T to C, chromosome 4 at 145,303,105 bp
  • A to T, chromosome 5 at 21,979,629 bp
  • A to T, chromosome 5 at 84,150,358 bp
  • C to T, chromosome 5 at 104,085,763 bp
  • A to G, chromosome 5 at 125,622,925 bp
  • T to C, chromosome 5 at 146,537,761 bp
  • C to G, chromosome 6 at 87,111,524 bp
  • C to T, chromosome 6 at 94,628,044 bp
  • T to C, chromosome 6 at 118,558,996 bp
  • T to A, chromosome 6 at 128,783,707 bp
  • G to A, chromosome 7 at 105,755,658 bp
  • G to T, chromosome 7 at 125,668,037 bp
  • C to T, chromosome 8 at 27,481,643 bp
  • A to G, chromosome 8 at 109,996,818 bp
  • A to T, chromosome 8 at 123,200,438 bp
  • G to A, chromosome 9 at 108,301,451 bp
  • C to T, chromosome 10 at 120,105,743 bp
  • T to C, chromosome 10 at 129,705,508 bp
  • C to T, chromosome 11 at 69,825,390 bp
  • A to G, chromosome 11 at 87,589,245 bp
  • G to T, chromosome 11 at 96,043,460 bp
  • CAGAAGAAG to CAGAAG, chromosome 12 at 24,612,179 bp
  • T to C, chromosome 12 at 36,024,066 bp
  • A to T, chromosome 12 at 83,771,882 bp
  • A to G, chromosome 12 at 114,060,090 bp
  • G to T, chromosome 13 at 4,194,169 bp
  • A to G, chromosome 13 at 33,832,485 bp
  • C to A, chromosome 14 at 26,850,926 bp
  • A to T, chromosome 14 at 50,837,398 bp
  • T to C, chromosome 15 at 27,754,029 bp
  • A to G, chromosome 15 at 31,461,994 bp
  • A to G, chromosome 16 at 22,898,923 bp
  • C to A, chromosome 17 at 23,561,399 bp
  • T to C, chromosome 17 at 26,287,050 bp
  • A to C, chromosome 17 at 38,208,317 bp
  • T to C, chromosome 19 at 5,990,334 bp
  • A to G, chromosome 19 at 7,711,138 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5149 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042732-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.